All Blogs | Help
This Post
Permalink
URI
URI Label
Revisions
Export:
XML (With Files)
PNG Image
Archives
March 2012 (19)
February 2012 (1)
August 2011 (1)
May 2011 (40)
February 2011 (4)
November 2010 (17)
October 2010 (17)
September 2010 (26)
August 2010 (4)
April 2010 (45)
March 2010 (19)
December 2009 (5)
November 2009 (2)
September 2009 (11)
August 2009 (7)
July 2009 (2)
June 2009 (5)
March 2009 (1)
February 2009 (95)
December 2008 (6)
November 2008 (47)
October 2008 (56)
Sections
Data (136)
Materials (183)
Note (5)
Procedure (21)
Procedures (74)
Templates (11)
Material
Solution (150)
Powder (12)
Suspension (1)
Data Type
SAXS Lab (9)
SANS LOQ (5)
UV-VIS (11)
Sequence DNA (1)
UV-Vis (9)
SAXS Diamond (74)
SANS (22)
SANS SANS2d (5)
Procedure
UV-Vis (4)
SANS (12)
SAXS Lab (1)
DNA Ligation (2)
Chemistry (1)
Data Analysis (2)
PCR (1)
DNA Phosphorylation (1)
SAXS (12)
Sample Preparation (1)
Data Analysis (6)
Transformation (1)
Protein Purification (5)
SDS PAGE (4)
Sample Preparation (4)
Data Reduction (4)
Protein Purification (4)
Synchrotron CD (3)
Purification (4)
Instrument
LOQ (7)
SAXSess Bath (1)
I22 (84)
SANS2d (7)
SANS2D (21)
Dna
Oligonucleotide (12)
Double Stranded Linear (4)
Plasmid (1)
Glur0 (2)
Project
Laser Tweezers Tus (6)
Mthk (12)
Kcsa (1)
Reaction Centre (2)
SAS Standard (27)
GluR0 (16)
Tus Labelling (6)
JHL (6)
Soton (26)
MurM (2)
Test (1)
Data Analysis (1)
Glur0 (48)
Frey-King Surfactants (1)
SANS2dfirstbio (9)
Pins (1)
GlurO (1)
Protein-ligation (3)
Smalp (40)
NIMROD-March-2012 (1)
Tools
Show/Hide QR Code
Show/Hide Keys
9th October 2008 @ 19:11
The pdh files generated in SAXS of GFP Samples were all binned from Q = 0.08 - 20 nm-1 to generate the associated bin files. The background SAXS Run 02067 was subtracted from all 30 minute sample runs to generate the associated sub files.

The rebinned data from SAXS Run 02067 and SAXS Run 02075 were summed to give 02075.add. This was used as the background for the 60 minute runs (SAXS Run 02071 and SAXS Run 02074) as well as for the sum of two runs of GFP solution 5 mg/ml designated 02070.add

All data files are provided at:

http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10

The background subtracted data were re-binned to generate the files designated #####b.bin. Binning range was selected to run from 0.25 nm-1 to where the scattering was deemed to reach noise levels (can be seen from the data files). The binned files were de-smeared using the provided Lake software with default parameters. These generated the associated des files.

All re-binned and de-smeared data files are provided at:

http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10

The data from the 1mg/mL solution SAXS Run 02070 was not desmeared as after background subtraction it had very little signal.
This Post is Linked By: Maria's analysis of the GFP SAXS data;
Comments
Re: GFP SAXS data reduction by Cameron Neylon
10th October 2008 @ 14:58
The reason why run 02070 had several points below zero after subtraction is because 02070 was a single 1 hour run (one set of electronic background) and the background substracted was the sum of two runs (two electronic background). These will be reprocessed and the re-run described in another post.