7575Lab SAXS Template
Templates
cameron.neylon.myopenid.comCameron Neylon

SampleExposure time (min)Run #Data
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]]>2008-10-09T11:22:07+01:002008-10-09T11:22:07+01:005b5a2b3c856a5b282ebaf1c6677c98ff5http://biolab.isis.rl.ac.uk/uri/20http://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.xml?revision=758181GFP solution 5 mg/ml
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A 5mg/mL solution of GFP in Sortase Buffer prepared in Preparation of GFP solutions
This Post is Linked By: Preparation of GFP solutions;GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T11:32:10+01:002008-10-09T11:32:10+01:00503b54a3da0d896d20d6c1388c15d954aSolutionhttp://biolab.isis.rl.ac.uk/uri/25http://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.xml?revision=818282GFP solution 2 mg/ml
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A 2mg/mL solution of GFP in Sortase Buffer prepared in Preparation of GFP solutions
This Post is Linked By: Preparation of GFP solutions; ]]>2008-10-09T11:33:25+01:002008-10-09T11:33:25+01:0053f3fac49af43fadb082ddb4b0253bd27Solutionhttp://biolab.isis.rl.ac.uk/uri/26http://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.xml?revision=828383GFP solution 1 mg/ml
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A 2mg/mL solution of GFP in Sortase Buffer prepared in Preparation of GFP solutions
This Post is Linked By: Preparation of GFP solutions;SAXS of GFP Samples; ]]>2008-10-09T12:03:10+01:002008-10-09T12:03:10+01:005f4b3e95420fd15c7d322aba34d053fc9Solutionhttp://biolab.isis.rl.ac.uk/uri/27http://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.xml?revision=837984Preparation of GFP solutions
Procedures
cameron.neylon.myopenid.comCameron Neylon
9.7 mg of Freeze dried GFP was resuspended in 970 uL of Sortase Buffer to yield GFP solution 10 mg/mL.

This solution was then diluted with Sortase Buffer to yield:

GFP solution 5 mg/ml
GFP solution 2 mg/ml
GFP solution 1 mg/ml
This Post is Linked By: GFP solution 5 mg/ml;GFP solution 2 mg/ml;GFP solution 1 mg/ml;GFP solution 10 mg/mL; ]]>2008-10-09T11:31:01+01:002008-10-09T12:04:55+01:00547825913e13aca36e785087a55032097http://biolab.isis.rl.ac.uk/uri/24http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.xml?revision=79http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.xml?revision=848686Centrifuged 10 mg/mL GFP
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
The centrifuged product of Centrifugation of 10 mg/mL GFP solution
This Post is Linked By: Centrifugation of 10 mg/mL GFP solution;SAXS of GFP Samples; ]]>2008-10-09T16:06:36+01:002008-10-09T16:06:36+01:005a0dcc82ec201f32d805c81ddb4744fbaSolutionhttp://biolab.isis.rl.ac.uk/uri/29http://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.xml?revision=868787Centrifuged 2 mg/mL GFP
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Spun solution of GFP at 2 mg/mL from Centrifugation of 10 mg/mL GFP solution
This Post is Linked By: Centrifugation of 10 mg/mL GFP solution;SAXS of GFP Samples; ]]>2008-10-09T16:08:11+01:002008-10-09T16:08:11+01:0057e46e48632324368bbcf9e798e04c9c8Solutionhttp://biolab.isis.rl.ac.uk/uri/2ahttp://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.xml?revision=878888Centrifuged 5 mg/mL GFP
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Spun solution of GFP at 5 mg/mL from Centrifugation of 10 mg/mL GFP solution
This Post is Linked By: Centrifugation of 10 mg/mL GFP solution;SAXS of GFP Samples; ]]>2008-10-09T16:09:03+01:002008-10-09T16:09:03+01:005eba9f8e012fb65668fcb5a5c0aa7f658Solutionhttp://biolab.isis.rl.ac.uk/uri/2bhttp://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.xml?revision=88110114SAXS Run 02075
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T18:16:04+01:002008-10-09T18:21:56+01:0055226880bb4b88fa8caf705aa0ef1f030SAXS_Labhttp://biolab.isis.rl.ac.uk/uri/36http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.xml?revision=110http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.xml?revision=114108115SAXS Run 02074
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T18:14:04+01:002008-10-09T18:22:15+01:00503f2e16e6153cd78718819a4d4523a46SAXS_Labhttp://biolab.isis.rl.ac.uk/uri/34http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.xml?revision=108http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.xml?revision=11597116SAXS Run 02073
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: SAXS of GFP Samples; ]]>2008-10-09T16:16:15+01:002008-10-09T18:22:29+01:005453bd92341a5e66790fed62e7a739f1aSAXS_Labhttp://biolab.isis.rl.ac.uk/uri/33http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.xml?revision=97http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.xml?revision=11696117SAXS Run 02072
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: SAXS of GFP Samples; ]]>2008-10-09T16:16:04+01:002008-10-09T18:22:48+01:00593138f5668a95d90a7580769cc3193a2SAXS_Labhttp://biolab.isis.rl.ac.uk/data/2.xmlhttp://biolab.isis.rl.ac.uk/uri/32http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xml?revision=96http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xml?revision=98http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xml?revision=99http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xml?revision=11795118SAXS Run 02071
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T16:15:47+01:002008-10-09T18:23:03+01:005da3545253c5f94d524e931ded2fa12fcSAXS_Labhttp://biolab.isis.rl.ac.uk/data/4.xmlhttp://biolab.isis.rl.ac.uk/uri/31http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xml?revision=95http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xml?revision=100http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xml?revision=101http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xml?revision=11894119SAXS Run 02070
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T16:15:35+01:002008-10-09T18:23:18+01:005da627ffbde618aaee605f54973001401SAXS_Labhttp://biolab.isis.rl.ac.uk/data/6.xmlhttp://biolab.isis.rl.ac.uk/uri/30http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xml?revision=94http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xml?revision=102http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xml?revision=103http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xml?revision=11993120SAXS Run 02069
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The processing was aborted because the image plate was misplaced on the reader.
This Post is Linked By: SAXS of GFP Samples; ]]>2008-10-09T16:15:23+01:002008-10-09T18:23:32+01:0050b17c1565dd38cb842a102bdbcda63ffSAXS_Labhttp://biolab.isis.rl.ac.uk/uri/2fhttp://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.xml?revision=93http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.xml?revision=104http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.xml?revision=12092121SAXS Run 02068
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: SAXS of GFP Samples; ]]>2008-10-09T16:15:11+01:002008-10-09T18:23:47+01:005bfac81fb3e17a826ef3316bfd8886095SAXS_Labhttp://biolab.isis.rl.ac.uk/data/8.xmlhttp://biolab.isis.rl.ac.uk/uri/2ehttp://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.xml?revision=92http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.xml?revision=105http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.xml?revision=12191122SAXS Run 02067
Data
cameron.neylon.myopenid.comCameron Neylon-1.]]>Data Type: SAXS_Lab
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm-1.
This Post is Linked By: GFP SAXS data reduction;SAXS of GFP Samples; ]]>2008-10-09T16:14:13+01:002008-10-09T18:24:49+01:005e0499d02179261a070388613e744bb55SAXS_Labhttp://biolab.isis.rl.ac.uk/data/10.xmlhttp://biolab.isis.rl.ac.uk/uri/2dhttp://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xml?revision=91http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xml?revision=106http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xml?revision=107http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xml?revision=122123128GFP SAXS data reduction
Procedures
cameron.neylon.myopenid.comCameron Neylon-1 to generate the associated bin files. The background [blog]91[/blog] was subtracted from all 30 minute sample runs to generate the associated sub files. The rebinned data from [blog]91[/blog] and [blog]110[/blog] were summed to give 02075.add. This was used as the background for the 60 minute runs ([blog]95[/blog] and [blog]108[/blog]) as well as for the sum of two runs of [blog]81[/blog] designated 02070.add All data files are provided at: http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10 The background subtracted data were re-binned to generate the files designated #####b.bin. Binning range was selected to run from 0.25 nm-1 to where the scattering was deemed to reach noise levels (can be seen from the data files). The binned files were de-smeared using the provided Lake software with default parameters. These generated the associated des files. All re-binned and de-smeared data files are provided at: http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10 The data from the 1mg/mL solution [blog]94[/blog] was not desmeared as after background subtraction it had very little signal.]]>SAXS of GFP Samples were all binned from Q = 0.08 - 20 nm-1 to generate the associated bin files. The background SAXS Run 02067 was subtracted from all 30 minute sample runs to generate the associated sub files.

The rebinned data from SAXS Run 02067 and SAXS Run 02075 were summed to give 02075.add. This was used as the background for the 60 minute runs (SAXS Run 02071 and SAXS Run 02074) as well as for the sum of two runs of GFP solution 5 mg/ml designated 02070.add

All data files are provided at:

http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10

The background subtracted data were re-binned to generate the files designated #####b.bin. Binning range was selected to run from 0.25 nm-1 to where the scattering was deemed to reach noise levels (can be seen from the data files). The binned files were de-smeared using the provided Lake software with default parameters. These generated the associated des files.

All re-binned and de-smeared data files are provided at:

http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10

The data from the 1mg/mL solution SAXS Run 02070 was not desmeared as after background subtraction it had very little signal.
This Post is Linked By: Maria's analysis of the GFP SAXS data; ]]>2008-10-09T19:11:32+01:002008-10-10T14:57:10+01:005f058512dd6813c498098b957e07ea341http://biolab.isis.rl.ac.uk/uri/37http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=123http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=124http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=125http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=126http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=127http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml?revision=12885129Centrifugation of 10 mg/mL GFP solution
Procedures
cameron.neylon.myopenid.comCameron NeylonCentrifuged 10 mg/mL GFP

From this solution fresh solutions of Centrifuged 5 mg/mL GFP and Centrifuged 2 mg/mL GFP
This Post is Linked By: Centrifuged 10 mg/mL GFP;Centrifuged 2 mg/mL GFP;Centrifuged 5 mg/mL GFP; ]]>2008-10-09T16:06:16+01:002008-10-13T13:38:51+01:005093a2979502fbd4116f64a554b0127b5http://biolab.isis.rl.ac.uk/uri/28http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.xml?revision=85http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.xml?revision=89http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.xml?revision=129131131GFP (10 mg/mL) in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A 10 mg/mL solution of GFP in D2O buffer prepared in Preparation of GFP Samples for a quickie SANS experiment on LOQ
This Post is Linked By: Preparation of GFP Samples for a quickie SANS experiment on LOQ;UV-Vis spectroscopy of GFP and Lysozyme samples;SANS on GFP concentration series;Comparison of SANS2d and LOQ GFP data; ]]>2008-10-16T12:15:41+01:002008-10-16T12:15:41+01:005c28ba56169ef8c61b8cc7618ee879c21Solutionhttp://biolab.isis.rl.ac.uk/uri/39http://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.xml?revision=131132132GFP 5 mg/mL in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A 5 mg/mL solution of GFP in D2O phophate buffer prepared in Preparation of GFP Samples for a quickie SANS experiment on LOQ
This Post is Linked By: Preparation of GFP Samples for a quickie SANS experiment on LOQ;UV-Vis spectroscopy of GFP and Lysozyme samples;SANS on GFP concentration series; ]]>2008-10-16T12:51:18+01:002008-10-16T12:51:18+01:005ba50ac4c30f1e4327942358befc3d09bSolutionhttp://biolab.isis.rl.ac.uk/uri/3ahttp://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.xml?revision=132133133GFP 2 mg/mL in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
An approx 2mg/mL solution of GFP in D2O phosphate buffer prepared in
This Post is Linked By: Preparation of GFP Samples for a quickie SANS experiment on LOQ;UV-Vis spectroscopy of GFP and Lysozyme samples;SANS on GFP concentration series; ]]>2008-10-16T12:51:55+01:002008-10-16T12:51:55+01:0054f8c138e5699553c08d251674adfa964Solutionhttp://biolab.isis.rl.ac.uk/uri/3bhttp://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.xml?revision=133130134Preparation of GFP Samples for a quickie SANS experiment on LOQ
Procedures
cameron.neylon.myopenid.comCameron NeylonFreeze dried GFP (10.5 mg) was disolved in 1.00 mL of 20 mM phosphate buffer in D2O. After addition the tube and contents weighed 1.1010g. The solution was then spun for 10' (20k x g) (to give GFP (10 mg/mL) in D2O buffer.

The solution was then diluted (300 uL + 300 uL buffer) to give GFP 5 mg/mL in D2O buffer and then this solution was diluted further (100 uL + 150 uL of buffer) to give GFP 2 mg/mL in D2O buffer.
This Post is Linked By: GFP (10 mg/mL) in D2O buffer;GFP 5 mg/mL in D2O buffer; ]]>2008-10-16T12:15:05+01:002008-10-16T12:55:24+01:0058d73b9766fc7ba43334947ede0adbc56http://biolab.isis.rl.ac.uk/uri/38http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.xml?revision=130http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.xml?revision=134135135Lysozyme
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Fisher lysozyme powder
This Post is Linked By: Preparation of lysozyme solutions for quickie SANS experiment; ]]>2008-10-16T15:13:20+01:002008-10-16T15:13:20+01:005494b31358edd5c631b5e38876000a1e2Powderhttp://biolab.isis.rl.ac.uk/uri/3chttp://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.xml?revision=135137137Lysozyme 10 mg/mL in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
10 mg/mL solution of lysozyme in D2O buffer preapred inPreparation of lysozyme solutions for quickie SANS experiment
This Post is Linked By: Preparation of lysozyme solutions for quickie SANS experiment;UV-Vis spectroscopy of GFP and Lysozyme samples;SANS on GFP concentration series; ]]>2008-10-16T15:15:44+01:002008-10-16T15:15:44+01:005f6aeee02449034bd4ed2d71f187f94baSolutionhttp://biolab.isis.rl.ac.uk/uri/3ehttp://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.xml?revision=137138138Lysozyme 2mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
2 mg/mL solution of lysozyme prepared in Preparation of lysozyme solutions for quickie SANS experiment
This Post is Linked By: Preparation of lysozyme solutions for quickie SANS experiment;UV-Vis spectroscopy of GFP and Lysozyme samples;SANS on GFP concentration series; ]]>2008-10-16T15:16:25+01:002008-10-16T15:16:25+01:0057b680eecebc1d358b07a5b224ea3c577Solutionhttp://biolab.isis.rl.ac.uk/uri/3fhttp://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.xml?revision=138136139Preparation of lysozyme solutions for quickie SANS experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonLysozyme (17.6 mg) was dissolved in D2O phophate buffer (880 uL) to give a 20 mg/mL solution. The solution was diluted (400uL + 400 uL buffer) to give Lysozyme 10 mg/mL in D2O buffer. This solution was diluted (100 uL + 400 uL of buffer) to give Lysozyme 2mg/mL.
This Post is Linked By: Lysozyme 10 mg/mL in D2O buffer;Lysozyme 2mg/mL; ]]>2008-10-16T15:15:30+01:002008-10-16T15:17:23+01:00565982a5385ead0c16f723448ee425099http://biolab.isis.rl.ac.uk/uri/3dhttp://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.xml?revision=136http://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.xml?revision=13914114120 mM Phosphate buffer in D2O
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Made by Luke
This Post is Linked By: SANS on GFP concentration series; ]]>2008-10-16T15:52:45+01:002008-10-16T15:52:45+01:005c92a79433ae5ced830a7003d2afae744Solutionhttp://biolab.isis.rl.ac.uk/uri/41http://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.xml?revision=141171171UV-Vis spectroscopy of GFP and Lysozyme samples
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: UV-Vis
Samples were run in 1mm banjo cells that were used for the SANS experiment. The samples were held in place by white-tac. The JASCO UV-Vis spectrophotometer was zeroed with no samples and then the baseline checked and re-zeroed with two buffer samples. Spectra were recorded with buffer in the reference position.


SampleData
GFP (10 mg/mL) in D2O bufferUV-Vis spectrum of 10mg/mL GFP
GFP 5 mg/mL in D2O bufferUV-Vis spectrum of 5mg/mL GFP
GFP 2 mg/mL in D2O bufferUV-Vis spectrum of 2mg/mL GFP
Lysozyme 10 mg/mL in D2O bufferUV-Vis spectrum of 10mg/mL Lysozyme
Lysozyme 2mg/mLUV-Vis spectrum of 2 mg/mL Lysozyme


]]>2008-10-20T11:32:11+01:002008-10-20T11:32:11+01:005c759aa20b21936714c6ffa55b0a80402UV-Vishttp://biolab.isis.rl.ac.uk/uri/48http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.xml?revision=17190173LOQ SANS data template
Templates
cameron.neylon.myopenid.comCameron NeylonData]] [[Data_type>SANS_LOQ]]]]>
[[Section>Data]]
[[Data_type>SANS_LOQ]]
]]>2008-10-09T16:12:42+01:002008-10-20T11:39:49+01:0053cb28ca8e67dcbca93ec449dff3c70d7http://biolab.isis.rl.ac.uk/uri/2chttp://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.xml?revision=90http://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.xml?revision=17317417744700 - 10mg/mL GFP on LOQ
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SANS_LOQ
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
This Post is Linked By: Initial analysis of SANS data on GFP;SANS on GFP concentration series; ]]>2008-10-20T11:40:22+01:002008-10-20T11:41:14+01:005636ca1234b3d40afab26dd24f5a9d00aSANS_LOQhttp://biolab.isis.rl.ac.uk/data/32.xmlhttp://biolab.isis.rl.ac.uk/data/34.xmlhttp://biolab.isis.rl.ac.uk/uri/49http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xml?revision=174http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xml?revision=175http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xml?revision=176http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xml?revision=17717818144701 - 5mg/mL GFP on LOQ
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SANS_LOQ
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
This Post is Linked By: Initial analysis of SANS data on GFP;SANS on GFP concentration series; ]]>2008-10-20T12:02:57+01:002008-10-20T12:03:44+01:005dbdad992d82be85e0141eab767f16f1eSANS_LOQhttp://biolab.isis.rl.ac.uk/data/36.xmlhttp://biolab.isis.rl.ac.uk/data/38.xmlhttp://biolab.isis.rl.ac.uk/uri/4ahttp://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xml?revision=178http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xml?revision=179http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xml?revision=180http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xml?revision=18118218544702 - 2mg/mL GFP on LOQ
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SANS_LOQ
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
This Post is Linked By: Initial analysis of SANS data on GFP;SANS on GFP concentration series; ]]>2008-10-20T12:03:58+01:002008-10-20T12:04:36+01:00598f3e53a69da806ea061880400784168SANS_LOQhttp://biolab.isis.rl.ac.uk/data/40.xmlhttp://biolab.isis.rl.ac.uk/data/42.xmlhttp://biolab.isis.rl.ac.uk/uri/4bhttp://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xml?revision=182http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xml?revision=183http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xml?revision=184http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xml?revision=18518618944703 - 10mg/mL Lysozyme on LOQ
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SANS_LOQ
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
This Post is Linked By: Initial analysis of SANS data on GFP;SANS on GFP concentration series;Comparison of SANS2d and LOQ Lysozyme data; ]]>2008-10-20T12:04:54+01:002008-10-20T12:05:30+01:00531a2c7d9fc3e6742ce67131d39e0fb6dSANS_LOQhttp://biolab.isis.rl.ac.uk/data/44.xmlhttp://biolab.isis.rl.ac.uk/data/46.xmlhttp://biolab.isis.rl.ac.uk/uri/4chttp://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xml?revision=186http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xml?revision=187http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xml?revision=188http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xml?revision=18919019344704 - 2mg/mL Lysozyme on LOQ
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SANS_LOQ
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
This Post is Linked By: Initial analysis of SANS data on GFP;SANS on GFP concentration series; ]]>2008-10-20T12:05:54+01:002008-10-20T12:06:41+01:005ca76931b8e905da561905fa5d06c4a7aSANS_LOQhttp://biolab.isis.rl.ac.uk/data/48.xmlhttp://biolab.isis.rl.ac.uk/data/50.xmlhttp://biolab.isis.rl.ac.uk/uri/4dhttp://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xml?revision=190http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xml?revision=191http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xml?revision=192http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xml?revision=193195197Initial analysis of SANS data on GFP
Procedures
cameron.neylon.myopenid.comCameron Neylon44700 - 10mg/mL GFP on LOQ, 44701 - 5mg/mL GFP on LOQ, and 44703 - 10mg/mL Lysozyme on LOQ was fairly good over the range of Q=0.01 - 0.1. Data from 44702 - 2mg/mL GFP on LOQ was ok and 44704 - 2mg/mL Lysozyme on LOQ wasn't really useable.

Guiner plots gave an Rg of ~24 A for GFP and the experimental data was well fitted by that generated from the pdb file 1gfl (http://www.pdb.org/pdb/explore/explore.do?structureId=1GFL) which is of a dimer. The Rg is consistent with a dimer. Trying to fit the data to that predicted from the monomer (prepared by deleting one chain from 1gfl) gave a very poor fit.

The lysozyme data is not fit well by data predicted from 2vb1 (http://www.pdb.org/pdb/explore.do?structureId=2VB1)
]]>2008-10-20T14:06:35+01:002008-10-20T14:07:15+01:005237204accab398d73eaeb9465c41bf84http://biolab.isis.rl.ac.uk/data/52.xmlhttp://biolab.isis.rl.ac.uk/uri/4ehttp://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.xml?revision=195http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.xml?revision=196http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.xml?revision=197156199UV-Vis spectrum of 2 mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-VIS
jws, jcamp, txt
This Post is Linked By: UV-Vis spectroscopy of GFP and Lysozyme samples; ]]>2008-10-17T12:18:30+01:002008-10-20T16:03:03+01:005af8d31033d76a2b1398cc724c5091f1dUV-VIShttp://biolab.isis.rl.ac.uk/data/20.xmlhttp://biolab.isis.rl.ac.uk/data/22.xmlhttp://biolab.isis.rl.ac.uk/data/54.xmlhttp://biolab.isis.rl.ac.uk/uri/47http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=156http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=157http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=158http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=159http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=160http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=198http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml?revision=199153201UV-Vis spectrum of 10mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-VIS
jws, jcamp, txt
This Post is Linked By: UV-Vis spectroscopy of GFP and Lysozyme samples; ]]>2008-10-17T12:17:34+01:002008-10-20T16:03:42+01:005458ca9609dde1fa076af9252c6eda454UV-VIShttp://biolab.isis.rl.ac.uk/data/18.xmlhttp://biolab.isis.rl.ac.uk/data/24.xmlhttp://biolab.isis.rl.ac.uk/data/56.xmlhttp://biolab.isis.rl.ac.uk/uri/46http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=153http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=154http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=155http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=161http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=162http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=169http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=200http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml?revision=201150203UV-Vis spectrum of 2mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-VIS
jws file, jcamp, txt
This Post is Linked By: UV-Vis spectroscopy of GFP and Lysozyme samples; ]]>2008-10-17T12:16:37+01:002008-10-20T16:04:13+01:005a4bd306a1bded4d1206b63a98a2c726dUV-VIShttp://biolab.isis.rl.ac.uk/data/16.xmlhttp://biolab.isis.rl.ac.uk/data/26.xmlhttp://biolab.isis.rl.ac.uk/data/58.xmlhttp://biolab.isis.rl.ac.uk/uri/45http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=150http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=151http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=152http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=163http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=164http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=202http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml?revision=203147205UV-Vis spectrum of 5mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-VIS
jws file, jcamp,txt
This Post is Linked By: UV-Vis spectroscopy of GFP and Lysozyme samples; ]]>2008-10-17T12:14:10+01:002008-10-20T16:04:46+01:005100180e9674e46e91befdc98268451e3UV-VIShttp://biolab.isis.rl.ac.uk/data/14.xmlhttp://biolab.isis.rl.ac.uk/data/28.xmlhttp://biolab.isis.rl.ac.uk/data/60.xmlhttp://biolab.isis.rl.ac.uk/uri/44http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=147http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=148http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=149http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=165http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=166http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=204http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml?revision=205144207UV-Vis spectrum of 10mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-VIS
JWS file, jcamp, txt
This Post is Linked By: UV-Vis spectroscopy of GFP and Lysozyme samples; ]]>2008-10-17T12:10:13+01:002008-10-20T16:05:22+01:005f5743fea2f674a7a7f8ce832fefff1f4UV-VIShttp://biolab.isis.rl.ac.uk/data/12.xmlhttp://biolab.isis.rl.ac.uk/data/30.xmlhttp://biolab.isis.rl.ac.uk/data/62.xmlhttp://biolab.isis.rl.ac.uk/uri/43http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=144http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=145http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=146http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=167http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=168http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=170http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=206http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml?revision=207142208SANS on GFP concentration series
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length banjo cells cell at a temperature of 20 C. The data were reduced using Collette and the buffer as a background subtraction.


SampleExposure time (min)Run #Trans Run#Data
20 mM Phosphate buffer in D2O604469944687
GFP (10 mg/mL) in D2O buffer60447004468844700 - 10mg/mL GFP on LOQ
GFP 5 mg/mL in D2O buffer60447014468944701 - 5mg/mL GFP on LOQ
GFP 2 mg/mL in D2O buffer60447024469044702 - 2mg/mL GFP on LOQ
Lysozyme 10 mg/mL in D2O buffer60447034469144703 - 10mg/mL Lysozyme on LOQ
Lysozyme 2mg/mL60447044469244704 - 2mg/mL Lysozyme on LOQ

]]>2008-10-16T15:52:49+01:002008-10-20T16:11:52+01:005676a9e02de155a7fe68dc681a1c28852SANSLOQhttp://biolab.isis.rl.ac.uk/uri/42http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml?revision=142http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml?revision=143http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml?revision=172http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml?revision=194http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml?revision=208109209SAXS of GFP Samples
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS_Lab
Instrument: SAXSess_Bath
The following samples were run as described on the SAXSess instrument in the Edler group at the University of Bath. The samples were run in the capillary cell at a temperature of 25 C. Data was collected on the image plate provided and read using ImageQuant software provided with the image plate reader. The image was converted to 1D scattering data (I vs Q) using the SAXS Quant software provided.


SampleExposure time (min)Run #Data
Sortase Buffer3002067SAXS Run 02067
GFP solution 5 mg/ml3002068SAXS Run 02068
GFP solution 5 mg/ml3002069SAXS Run 02069
GFP solution 5 mg/ml3002070SAXS Run 02070
GFP solution 1 mg/ml6002071SAXS Run 02071
Centrifuged 10 mg/mL GFP3002072SAXS Run 02072
Centrifuged 5 mg/mL GFP3002073SAXS Run 02073
Centrifuged 2 mg/mL GFP6002074SAXS Run 02074
Sortase Buffer3002075SAXS Run 02075


Run #02069 was aborted because the image plate was misaligned in the image plate carousel. There is therefore no data file recorded.
This Post is Linked By: GFP SAXS data reduction; ]]>2008-10-09T18:14:20+01:002008-10-20T16:12:50+01:00578a2af9140bece87c082f8e2ac1c874dSAXS_LabSAXSess_Bathhttp://biolab.isis.rl.ac.uk/uri/35http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml?revision=109http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml?revision=111http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml?revision=112http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml?revision=113http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml?revision=209210217oligo template
Templates
cameron.neylon.myopenid.comCameron NeylonMaterials]] [[DNA>oligonucleotide]] [[Material>solution]]]]>PropertyDataName[[box]]Number[[box]]Location[[box]]Sequence[[box]]Length[[box]]Melting temp[[box]]Supplier[[box]]Stock concentration[[box]]

[[Section>Materials]]
[[DNA>oligonucleotide]]
[[Material>solution]]
]]>2008-10-27T13:09:49+00:002008-10-27T13:45:50+00:0051638fb8d63ae6aac1aabc45f61eb59edhttp://biolab.isis.rl.ac.uk/uri/4fhttp://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.xml?revision=210http://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.xml?revision=217231234Planning preparation of DNA for Laser Tweezers experiment
Note
cameron.neylon.myopenid.comCameron Neylon-8 M as required by the described method. Unfortunately it is in a Tris-EDTA buffer which will probably interfere with the kinase and ligation reactions. I will therefore take 100 uL and ethanol ppt it as Luke has recently made up buffers for this. The ligation of the first (biotin end) then follows by mixing the lambda (10-8 M) with [blog]224[/blog] (10-7 M) and annealing followed by ligation. This mixture is then combined with the appropriate combination of end oligos ([blog]221[/blog] and [blog]218[/blog] or [blog]214[/blog] and[blog]211[/blog] at 10-6 M) followed by annealing and ligation. As the amount of biotinylated lamda we want is relatively small contamination with the biotin oligo shouldn't be a problem. If this seems to cause issues we can easily ethanol ppt during the experiment. I think it will be advisable to get fresh PNK and ligase for this along with fresh ATP to rule out any problems with the enzymes. These are now ordered so we are right to go tomorrow or Thursday. Will attempt to sort out ethanol ppt of lambda this afternoon and make sure buffers are together.]]>http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/18571

As described the presumed final volume for the ligations reactions is the 50uL given in the supplementary information for this paper: PMID: 12947199. There is sufficient DNA for each of the oligos to make 100 uM stocks which will make it easy to add relatively small volumes for each ligation step.

The overall plan is therefore to make 100 uM stocks of the oligos in straight Tris buffer. For each oligo I will then do a kinase reaction at ~100 uM oligo (i.e. by just adding the reagents). The Lambda DNA is 0.33 ug/uL which is very close to 1x10-8 M as required by the described method. Unfortunately it is in a Tris-EDTA buffer which will probably interfere with the kinase and ligation reactions. I will therefore take 100 uL and ethanol ppt it as Luke has recently made up buffers for this.

The ligation of the first (biotin end) then follows by mixing the lambda (10-8 M) with Oligo-lambda-biotin (10-7 M) and annealing followed by ligation. This mixture is then combined with the appropriate combination of end oligos (Oligo-TerRlam and Oligo-TerR or Oligo-TerFlam andOligo-TerF at 10-6 M) followed by annealing and ligation.

As the amount of biotinylated lamda we want is relatively small contamination with the biotin oligo shouldn't be a problem. If this seems to cause issues we can easily ethanol ppt during the experiment.

I think it will be advisable to get fresh PNK and ligase for this along with fresh ATP to rule out any problems with the enzymes. These are now ordered so we are right to go tomorrow or Thursday. Will attempt to sort out ethanol ppt of lambda this afternoon and make sure buffers are together.
]]>2008-10-27T15:01:53+00:002008-10-28T12:12:57+00:00523e3763280db34015c5bce0c08b817e1http://biolab.isis.rl.ac.uk/uri/57http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xml?revision=231http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xml?revision=232http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xml?revision=233http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xml?revision=234240242Ethanol precipitated lambda DNA
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Lambda DNA resuspended in nuclease free water produced in Ethanol precipitation of lambda DNA
This Post is Linked By: Phosphorylation of DNA substrates for laser tweezers experiment;Ethanol precipitation of lambda DNA; ]]>2008-10-28T15:35:18+00:002008-10-28T15:37:39+00:005c07cb6713f9f2895994f7f1ff302d03cSolutionhttp://biolab.isis.rl.ac.uk/uri/5chttp://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.xml?revision=240http://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.xml?revision=242263263Phosphorylated TerR lam
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: oligonucleotide
Prepared in Phosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda;First preparation of beads for laser tweezers experiment;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:02:10+00:002008-11-05T16:02:10+00:0050f354bb608239d3e912774be394d33bfSolutionoligonucleotidehttp://biolab.isis.rl.ac.uk/uri/67http://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.xml?revision=263264264Phosporylated TerR
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: oligonucleotide
Prepared in Phosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:02:43+00:002008-11-05T16:02:43+00:005e06c845b4981f3a16964a83001feaacaSolutionoligonucleotidehttp://biolab.isis.rl.ac.uk/uri/68http://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.xml?revision=264265268Phosphorylated TerFlam
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: oligonucleotide
Prepared inPhosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:03:13+00:002008-11-05T16:04:45+00:0058349da83b21c84c3f50ddf46e73e8403Solutionoligonucleotidehttp://biolab.isis.rl.ac.uk/uri/69http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.xml?revision=265http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.xml?revision=268269269Phosphorylated oligo-lambda-biotin
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: oligonucleotide
prepared inPhosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of oligo-biotin-lambda to lambda DNA;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:05:58+00:002008-11-05T16:05:58+00:005a2fba022fee57b22f8a5616b1fcbc3b5Solutionoligonucleotidehttp://biolab.isis.rl.ac.uk/uri/6bhttp://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.xml?revision=269275275Product of ligation of lambda to lambda-oligo-biotin
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: double_stranded_linear
The product of Ligation of oligo-biotin-lambda to lambda DNA
This Post is Linked By: Ligation of oligo-biotin-lambda to lambda DNA;Ligation of Tus adaptors to biotin-modified lambda; ]]>2008-11-05T17:08:20+00:002008-11-05T17:08:20+00:005509e19551e60cfd8443d68879e69d98bSolutiondouble_stranded_linearhttp://biolab.isis.rl.ac.uk/uri/6fhttp://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.xml?revision=275262276Phosphorylated lambda DNA
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: double_stranded_linear
Phosphorylated lambda DNA prepared in Phosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of oligo-biotin-lambda to lambda DNA;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:01:05+00:002008-11-05T17:08:39+00:005da7fcf88bcd3ef5c45f38631f7c51124Solutiondouble_stranded_linearhttp://biolab.isis.rl.ac.uk/uri/66http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.xml?revision=262http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.xml?revision=276273277Ligation of oligo-biotin-lambda to lambda DNA
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: DNA_ligation
Phosphorylated lambda DNA (250 uL) and Phosphorylated oligo-lambda-biotin (0.5 uL) were combined and heated to 50 C followed by slow cooling over one hour. To this solution was added 5 x T4 DNA ligase buffer (1 uL) and the solution incubated overnight at 16 C to give Product of ligation of lambda to lambda-oligo-biotin.

This procedure follows that in PMID: 12947199 (supplementary information) except for a longer ligation period.
This Post is Linked By: Product of ligation of lambda to lambda-oligo-biotin; ]]>2008-11-05T17:06:34+00:002008-11-05T17:29:54+00:00505308b7374d0fab7fd0173ec769c3792DNA_ligationhttp://biolab.isis.rl.ac.uk/uri/6ehttp://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.xml?revision=273http://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.xml?revision=277266278Phosphorylated TerF
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: oligonucleotide
Prepared inPhosphorylation of DNA substrates for laser tweezers experiment
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-11-05T16:03:43+00:002008-11-05T17:30:52+00:00510a0a8b6e2dbc76f06e2ee4d176def7cSolutionoligonucleotidehttp://biolab.isis.rl.ac.uk/uri/6ahttp://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.xml?revision=266http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.xml?revision=267http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.xml?revision=278224279Oligo-lambda-biotin
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
Namelambda-biotin
Number
LocationFreezer 1 - Cameron's box
Sequence5'-Agg TCg CCg CCC AAA AAA AAA AAA AAA AAA AA-biotin-3'
Length32
Melting temp
SupplierInvitrogen
Stock concentration

This Post is Linked By: Planning preparation of DNA for Laser Tweezers experiment;Preparing fresh polymer-DNA beads for lasers experiment;Examining silica beads for stickiness to surface;Polymer beads with low loading of lambda-biotin-DNA;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-10-27T14:14:48+00:002008-11-05T17:31:13+00:00556e121e1f56bbe3139b745b48c8fb340oligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/72.xmlhttp://biolab.isis.rl.ac.uk/uri/54http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xml?revision=224http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xml?revision=225http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xml?revision=226http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xml?revision=279221280Oligo-TerRlam
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
NameTerRlam
Number
LocationFreezer 1 - Cameron's box
Sequenceggg Cgg CgA CCTATAAGTATGTTGTAACTAAAG
Length33
Melting temp
SupplierInvitrogen
Stock concentration

This Post is Linked By: Planning preparation of DNA for Laser Tweezers experiment;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-10-27T13:48:01+00:002008-11-05T17:31:31+00:005588f4a854c7099e2e04456d8b725724boligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/70.xmlhttp://biolab.isis.rl.ac.uk/uri/53http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xml?revision=221http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xml?revision=222http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xml?revision=223http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xml?revision=280218281Oligo-TerR
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
NameTerR
Number
LocationFreezer 1 - Cameron's box
SequenceCTTTAGTTACAACATACTTAT
Length21
Melting temp
SupplierInvitrogen
Stock concentration

This Post is Linked By: Planning preparation of DNA for Laser Tweezers experiment;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-10-27T13:46:50+00:002008-11-05T17:31:52+00:00568973ced2a72fc9864763b921e8ca7f3oligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/68.xmlhttp://biolab.isis.rl.ac.uk/uri/52http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xml?revision=218http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xml?revision=219http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xml?revision=220http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xml?revision=281214282Oligo-TerFlam
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
NameTerFlam
Number
LocationFreezer 1 - Cameron's box
Sequenceggg Cgg CgA CCT CTTTAGTTACAACATACTTAT
Length33
Melting temp
SupplierInvitrogen
Stock concentration

This Post is Linked By: Planning preparation of DNA for Laser Tweezers experiment;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-10-27T13:44:58+00:002008-11-05T17:32:16+00:005f16fbbd01b399a75fe1ba85af0520809oligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/66.xmlhttp://biolab.isis.rl.ac.uk/uri/51http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xml?revision=214http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xml?revision=215http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xml?revision=216http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xml?revision=282211283Oligo-TerF
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
NameTerF
Number
LocationFreezer 1 - Cameron's box
SequenceATAAGTATGTTGTAACTAAAG
Length21
Melting temp
SupplierInvitrogen
Stock concentration

This Post is Linked By: Planning preparation of DNA for Laser Tweezers experiment;Phosphorylation of DNA substrates for laser tweezers experiment; ]]>2008-10-27T13:41:00+00:002008-11-05T17:32:48+00:005c4720d14f49f328ae45fdd762ed50001oligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/64.xmlhttp://biolab.isis.rl.ac.uk/uri/50http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xml?revision=211http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xml?revision=212http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xml?revision=213http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xml?revision=28376284Sortase Buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Prepared by Maria Gomis-Gomis at the University of Southampton.


PropertyData
NameSortase buffer
Expiry
pH7.5
Tris-HCl50 mM
NaCl100 mM
CaCl25 mM

This Post is Linked By: GFP solution 5 mg/ml;Preparation of GFP solutions;SAXS of GFP Samples; ]]>2008-10-09T11:24:25+01:002008-11-05T17:33:08+00:005978a60a5043b41604e73716c574cc2b1Solutionhttp://biolab.isis.rl.ac.uk/uri/21http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.xml?revision=76http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.xml?revision=28478285GFP solution 10 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
The solution of GFP prepared in Preparation of GFP solutions
This Post is Linked By: Preparation of GFP solutions; ]]>2008-10-09T11:30:01+01:002008-11-05T17:34:43+00:0057693cecaf1ce2d82a892ca74c195973bSolutionhttp://biolab.isis.rl.ac.uk/uri/23http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.xml?revision=78http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.xml?revision=80http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.xml?revision=28577286Freeze dried GFP
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
GFP prepared by Luke Clifton and Mark Telling. This has been purified by Ni-NTA chromatography but not further purified. A fluffy yellow powder. This specific sample given to Maria Gomis-Gomis at Southampton University for SAXS and MS experiments
This Post is Linked By: Preparation of GFP solutions;Preparation of GFP Samples for a quickie SANS experiment on LOQ; ]]>2008-10-09T11:26:29+01:002008-11-05T17:35:00+00:00584824d7f45eb1244a656f88f09415addSolutionhttp://biolab.isis.rl.ac.uk/uri/22http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.xml?revision=77http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.xml?revision=2862872871:100 gly-OH modified silica beads
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Silica beads
This Post is Linked By: Ammonia treatment of gly modified beads; ]]>2008-11-06T10:22:35+00:002008-11-06T10:22:35+00:0056f68f1c89bca7a211164105aacae2089Powderhttp://biolab.isis.rl.ac.uk/uri/70http://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.xml?revision=2872882881:1000 gly-OH modified silica beads
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
beads
This Post is Linked By: Ammonia treatment of gly modified beads; ]]>2008-11-06T10:23:26+00:002008-11-06T10:23:26+00:0058ec756f325088ccba987840212c4a679Powderhttp://biolab.isis.rl.ac.uk/uri/71http://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.xml?revision=2882892891:10000 gly-OH modified silica beads
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
beads
This Post is Linked By: Ammonia treatment of gly modified beads; ]]>2008-11-06T10:23:50+00:002008-11-06T10:23:50+00:005a107536cd93fc6a1aa0d3b979a719ae4Powderhttp://biolab.isis.rl.ac.uk/uri/72http://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.xml?revision=2892902901:100000 gly-OH modified silica beads
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
beads
This Post is Linked By: Ammonia treatment of gly modified beads; ]]>2008-11-06T10:24:40+00:002008-11-06T10:24:40+00:005d1e966f54e417407af955c2dc52a3472Powderhttp://biolab.isis.rl.ac.uk/uri/73http://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.xml?revision=290295296TerF-lambda-biotin construct
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: double_stranded_linear
Product #1 of Ligation of Tus adaptors to biotin-modified lambda
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda; ]]>2008-11-06T11:28:21+00:002008-11-06T11:28:42+00:00533bf2c8eef376771d7b30be23d5e7f64Solutiondouble_stranded_linearhttp://biolab.isis.rl.ac.uk/uri/76http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.xml?revision=295http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.xml?revision=296294299Ligation of Tus adaptors to biotin-modified lambda
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: DNA_ligation
To Product of ligation of lambda to lambda-oligo-biotin was added the adaptors as given below. The solutions were heated to 65 C and then cooled over approximately one hour. Fresh ATP (0.5 uL of 10 mM) and T4 DNA ligase (1 uL of 1U/uL) were then added and the solutions incubated at 16 C for two hours.


#Product of ligation of lambda to lambda-oligo-biotin (uL)Adaptor 1uLAdaptor 2uLProduct
150Phosphorylated TerR0.5Phosphorylated TerRlam0.5TerF-lambda-biotin construct
250Phosporylated TerR0.5Phosphorylated TerR lam0.5TerR-lambda-biotin construct


Note that Rxn #1 is actually Ter forward. It shows as TerR because of a mistake with the original title of the material posts.
This Post is Linked By: TerF-lambda-biotin construct;TerR-lambda-biotin construct; ]]>2008-11-06T11:14:54+00:002008-11-06T11:48:48+00:005a5b9176047db8c0ca2bc781e947e7382DNA_ligationhttp://biolab.isis.rl.ac.uk/uri/75http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.xml?revision=294http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.xml?revision=297http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.xml?revision=2993003001:100 gly-oh beads after ammonia treatment
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Product #1 from Ammonia treatment of gly modified beads
This Post is Linked By: Fresh beads for Laser Tweezers experiments;First preparation of beads for laser tweezers experiment; ]]>2008-11-06T11:53:11+00:002008-11-06T11:53:11+00:005f622e865987f526ece6a435da4a796f4Powderhttp://biolab.isis.rl.ac.uk/uri/78http://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.xml?revision=3003013011:1000 gly-OH beads after ammonia treatment
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Product #2 of Ammonia treatment of gly modified beads
This Post is Linked By: Fresh beads for Laser Tweezers experiments; ]]>2008-11-06T11:53:45+00:002008-11-06T11:53:45+00:005b87b8f263f1a2342bb1067002630d241Powderhttp://biolab.isis.rl.ac.uk/uri/79http://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.xml?revision=3013023021:10000 gly-OH beads after ammonia treatment
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Product #3 ofAmmonia treatment of gly modified beads
This Post is Linked By: Fresh beads for Laser Tweezers experiments; ]]>2008-11-06T11:54:16+00:002008-11-06T11:54:16+00:005f3bc0ca8ce63072270074731be0e4468Powderhttp://biolab.isis.rl.ac.uk/uri/7ahttp://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.xml?revision=3023033031:100,000 gly-OH beads after ammonia treatment
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
product #4 of Ammonia treatment of gly modified beads
This Post is Linked By: Fresh beads for Laser Tweezers experiments; ]]>2008-11-06T11:54:46+00:002008-11-06T11:54:46+00:005b2df3625ee2d0541d159ff4a02353a66Powderhttp://biolab.isis.rl.ac.uk/uri/7bhttp://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.xml?revision=303291304Ammonia treatment of gly modified beads
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Chemistry
A sample of the silica beads previously prepared were treated with aqueous ammonia (200 uL) for 30' at 65 C as given.


BeadsWeight (mg)Product
1:100 gly-OH modified silica beads20.96
1:1000 gly-OH modified silica beads31.26
1:10000 gly-OH modified silica beads26.57
1:100000 gly-OH modified silica beads20.92


After addition of the ammonia the beads rapidly dispersed. A quick spin seemed to show there was still some beads there so hopefully not dissolved.

Beads were washed five times with ~1 mL of water and then dried in the freeze drier. Beads seem to be ok after washing.
This Post is Linked By: 1:100 gly-oh beads after ammonia treatment;1:1000 gly-OH beads after ammonia treatment;1:10000 gly-OH beads after ammonia treatment;1:100,000 gly-OH beads after ammonia treatment; ]]>2008-11-06T10:27:35+00:002008-11-06T12:16:50+00:005888f4662662c26dbcbf809531934fd52Chemistryhttp://biolab.isis.rl.ac.uk/uri/74http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xml?revision=291http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xml?revision=292http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xml?revision=293http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xml?revision=304305307Maria's analysis of the GFP SAXS data
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Data_analysis
Maria Gomis-Gomis has done some analysis of Rg and I(0) values from the SAXS data that is described in GFP SAXS data reduction.

The Rgs are broadly consistent with the SANS data for the 5mg/mL and 2 mg/mL samples. The I(0) values do not appear consistent suggesting issues with background subtraction that will need to be looked at.
]]>2008-11-07T11:23:34+00:002008-11-07T11:24:56+00:005d9c61b665d361af17a928f681ec1e400Data_analysishttp://biolab.isis.rl.ac.uk/data/76.xmlhttp://biolab.isis.rl.ac.uk/uri/7chttp://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.xml?revision=305http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.xml?revision=306http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.xml?revision=307298314TerR-lambda-biotin construct
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Dna: double_stranded_linear
Product #2 of Ligation of Tus adaptors to biotin-modified lambda
This Post is Linked By: Ligation of Tus adaptors to biotin-modified lambda;Preparing fresh polymer-DNA beads for lasers experiment;Examining silica beads for stickiness to surface;Polymer beads with low loading of lambda-biotin-DNA; ]]>2008-11-06T11:47:08+00:002008-11-11T15:48:39+00:005ce89fe18d50229b6e9d4b3e826e2d187Solutiondouble_stranded_linearhttp://biolab.isis.rl.ac.uk/uri/77http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.xml?revision=298http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.xml?revision=314352352Bangs Labs streptavidin coated beads
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Suspension
to be filled in
This Post is Linked By: Preparing fresh polymer-DNA beads for lasers experiment;First preparation of beads for laser tweezers experiment; ]]>2008-11-12T14:10:53+00:002008-11-12T14:10:53+00:005d8d96935b67d252c34e54ac780614dc2Suspensionhttp://biolab.isis.rl.ac.uk/uri/8fhttp://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.xml?revision=352375380Preparing fresh polymer-DNA beads for lasers experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Laser_tweezers_tus
Bangs Labs streptavidin coated beads (20 uL) were mixed with TerR-lambda-biotin construct (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl.

Bangs Labs streptavidin coated beads (20 uL) were mixed with TerR-lambda-biotin construct (1 uL) and Oligo-lambda-biotin (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl and then 3 x 100 mM NaCl.
]]>2008-11-14T10:21:15+00:002008-11-14T12:14:36+00:0058fd45d9d3700674ffcc570cf51f55a61Laser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/9ahttp://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.xml?revision=375http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.xml?revision=377http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.xml?revision=380379385Examining silica beads for stickiness to surface
Note
cameron.neylon.myopenid.comCameron NeylonProject: Laser_tweezers_tus
A quick check on labelled 100:1 beads and 100,000:1 beads indicate that both are very sticky when suspended in water or buffer. it seems possible to disperse them from the top and catch a few which may be viable for experiments but a bit of a pain.

More comprehensive analysis:

In 100 mM all appear to be sticky and also sticking to themselves. Beads are forming aggregates much worse than they were before.

All beads sets are extremely sticky in 100 mM, 50 mM, 0mM NaCl buffer. Beads themselves are more sticky, probably due to being shaken overnight in water but aggregates seem loose so sonication should clear things up. Not much good for easy interaction studies though.

Polymer beads when settled seem to be possible to pull back off the surface with the tweezers. After 10-15 minutes it is still possible to pull polymer beads (with DNA) off the surface with the traps. After 30 minutes it is getting harder to find easily trappable beads but there are still plenty about.

But there seems to be significant differences between different cells. Sticking in some but not others. Beads treated with TerR-lambda-biotin construct plus Oligo-lambda-biotin seem to be less prone to sticking than those just treated with TerR-lambda-biotin construct.
]]>2008-11-14T12:14:20+00:002008-11-14T17:12:14+00:005e7196b695b66f55c2c935b6480548205Laser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/9bhttp://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml?revision=379http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml?revision=382http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml?revision=383http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml?revision=384http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml?revision=385387388Octameric rck domain from MthK in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
Octameric rck domain in 20 mM Tris-pD 8.0, 250 mM NaCl, in D2O. Prepared by Luke by performing gel filtration at pH 8.0
This Post is Linked By: SANS runs on octameric RCK;Additional runs on octameric Rck;Addition of Ca++ to octameric mthk sample;Dialysis of Octameric RCK samples; ]]>2008-11-16T11:21:24+00:002008-11-16T11:21:52+00:0054b8da4794fd6123a894a6978b6fd1c1eSolutionmthkhttp://biolab.isis.rl.ac.uk/uri/9dhttp://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.xml?revision=387http://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.xml?revision=388140398SANS on LOQ template
Templates
cameron.neylon.myopenid.comCameron NeylonProcedures]] [[Procedure>SANS]] [[Instrument>LOQ]]]]>

SampleSANS/TRANSExposure time (uamp.hr)Run #Data
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]



[[Section>Procedures]]
[[Procedure>SANS]]
[[Instrument>LOQ]]
]]>2008-10-16T15:51:26+01:002008-11-16T12:42:38+00:0052d587f98ce20238e1ff3eb9cc54e04e2http://biolab.isis.rl.ac.uk/uri/40http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xml?revision=140http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xml?revision=392http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xml?revision=393http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xml?revision=398394401SANS on LOQ template
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure (uamp.hr)Run #Data
LOQ T49 Standard 14 mmT1046281
LOQ standard Can 14 mmT1046282
Direct beamT1046283
LOQ T49 Standard 14 mmS1546284
LOQ standard Can 14 mmS1546285

]]>2008-11-16T12:38:41+00:002008-11-16T12:45:55+00:005d8c995003b88a4bc3aae88cd07bcbfadSANSLOQhttp://biolab.isis.rl.ac.uk/data/82.xmlhttp://biolab.isis.rl.ac.uk/uri/9fhttp://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=394http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=395http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=396http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=397http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=399http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=400http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml?revision=401402404SANS runs on octameric RCK
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Octameric rck domain from MthK in D2O bufferT1046286
Buffer background for octameric rck domainT1046287
Octameric rck domain from MthK in D2O bufferS8546288
Buffer background for octameric rck domainS8546289

]]>2008-11-16T13:29:40+00:002008-11-16T13:30:12+00:005917c66865caf9ad4138621894c8e1b37SANSLOQhttp://biolab.isis.rl.ac.uk/data/84.xmlhttp://biolab.isis.rl.ac.uk/uri/a0http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.xml?revision=402http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.xml?revision=403http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.xml?revision=404405407Additional runs on octameric Rck
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Octameric rck domain from MthK in D2O bufferS8546290
Buffer background for octameric rck domainS8546291

]]>2008-11-16T15:57:06+00:002008-11-16T15:57:49+00:0058efc50f30b73885389040d482a9128a6SANSLOQhttp://biolab.isis.rl.ac.uk/data/86.xmlhttp://biolab.isis.rl.ac.uk/uri/a1http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.xml?revision=405http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.xml?revision=406http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.xml?revision=407409409Octameric RCK plus Ca
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
The product of adding Ca to octameric RCK domain in Addition of Ca++ to octameric mthk sample




This Post is Linked By: Addition of Ca++ to octameric mthk sample;SANS of octameric RCK plus Ca++;Dialysis of Octameric RCK samples; ]]>2008-11-16T17:38:22+00:002008-11-16T17:38:22+00:0056d655284c48c88bb74e99cd332bbfe14Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/a3http://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.xml?revision=409410410Buffer background for octameric RCK plus Ca
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
The product of adding Ca to buffer background in Addition of Ca++ to octameric mthk sample




This Post is Linked By: Addition of Ca++ to octameric mthk sample;SANS of octameric RCK plus Ca++; ]]>2008-11-16T17:40:07+00:002008-11-16T17:40:07+00:005a904b86e09cbd74c58109caa9a51b53dSolutionmthkhttp://biolab.isis.rl.ac.uk/uri/a4http://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.xml?revision=410408415Addition of Ca++ to octameric mthk sample
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: mthk
To each of Octameric rck domain from MthK in D2O buffer and Buffer background for octameric rck domain (500 uL) in 1 mm SANS Tank cells was added CaCl2 (15 uL of a 1 M solution) to give Octameric RCK plus Ca and Buffer background for octameric RCK plus Ca respectively.

Some precipitation was observed in the protein sample so it was spun down and returned to the sample cell.
This Post is Linked By: Octameric RCK plus Ca;Buffer background for octameric RCK plus Ca; ]]>2008-11-16T17:38:03+00:002008-11-16T18:09:23+00:0054c4aa4cb1d88ab8fc76d521b7a05611cmthkhttp://biolab.isis.rl.ac.uk/uri/a2http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.xml?revision=408http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.xml?revision=411http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.xml?revision=415417417Dimeric RCK from gel filtration in pD 6.5 D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
Dimeric RCK off gel filtration column in 20 mM MES 250 mM KCl, pD 6.5 in D2O




This Post is Linked By: SANS of dimeric RCK; ]]>2008-11-16T22:29:21+00:002008-11-16T22:29:21+00:00585d3f2c442509ac080bb968cc24b6614Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/a6http://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.xml?revision=417418418Buffer background for dimeric RCK
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
20 mM MES, 250 mM KCl, pD 6.5 in D2O




This Post is Linked By: SANS of dimeric RCK; ]]>2008-11-16T22:30:01+00:002008-11-16T22:30:01+00:0051d9b4e9ae5cc193c16ca5702f5135a17Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/a7http://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.xml?revision=418419421SANS of dimeric RCK
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Dimeric RCK from gel filtration in pD 6.5 D2O bufferT1046298
Buffer background for dimeric RCKT1046299
Dimeric RCK from gel filtration in pD 6.5 D2O bufferS8546300
Buffer background for dimeric RCKS8546301

]]>2008-11-16T22:31:38+00:002008-11-16T22:33:43+00:005052747bd64b94dcab3e7e37e44bd36baSANSLOQhttp://biolab.isis.rl.ac.uk/uri/a8http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.xml?revision=419http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.xml?revision=420http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.xml?revision=421412424SANS of octameric RCK plus Ca++
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Octameric RCK plus CaT1046292
Buffer background for octameric RCK plus CaT1046293
Octameric RCK plus CaS8546294
Buffer background for octameric RCK plus CaS8546295
Octameric RCK plus CaS8546296
Buffer background for octameric RCK plus CaS8546297


The order was changed after uploading the control script. The second control script is the correct one.
]]>2008-11-16T17:58:16+00:002008-11-16T22:35:41+00:005c6b49643ecf5e75bb27a4ef4c1c0d087SANSLOQhttp://biolab.isis.rl.ac.uk/data/88.xmlhttp://biolab.isis.rl.ac.uk/data/90.xmlhttp://biolab.isis.rl.ac.uk/uri/a5http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=412http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=413http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=414http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=416http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=422http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=423http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml?revision=424426426Octameric RCK plus Ca dialysed into pD6.5
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
Sample of octameric RCK with calcium added that was dialysed into pD 6.5 buffer in Dialysis of Octameric RCK samples




This Post is Linked By: Dialysis of Octameric RCK samples;SANS on LOQ template; ]]>2008-11-17T01:00:49+00:002008-11-17T01:00:49+00:0058e7951e3dfe66b3a87d348555db48232Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/aahttp://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.xml?revision=426427430Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
The sample of octameric RCK dialysed into 20 mM MES, 250 mM KCl pD 6.5
This Post is Linked By: Dialysis of Octameric RCK samples;SANS on LOQ template; ]]>2008-11-17T01:01:31+00:002008-11-17T01:04:25+00:0054e0f1a0bd9d97d5a37249bb6bf1f1e2bSolutionmthkhttp://biolab.isis.rl.ac.uk/uri/abhttp://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.xml?revision=427http://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.xml?revision=430425431Dialysis of Octameric RCK samples
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: mthk
The samples Octameric rck domain from MthK in D2O buffer and Octameric RCK plus Ca were dialysed into pD 6.5 buffer (MES, 250 mM KCl, one change) and the same buffer with 25 mM Ca2+ to give the samples Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer and Octameric RCK plus Ca dialysed into pD6.5 respectively.
This Post is Linked By: Octameric RCK plus Ca dialysed into pD6.5; ]]>2008-11-17T01:00:35+00:002008-11-17T01:04:46+00:005977adc83a6cee25d669b90417564a96emthkhttp://biolab.isis.rl.ac.uk/uri/a9http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xml?revision=425http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xml?revision=428http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xml?revision=429http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xml?revision=43143243220 mM MES pD6.5 250 mM KCl in D2O
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
Buffer pD 6.5 with no Ca2+




This Post is Linked By: SANS on LOQ template; ]]>2008-11-17T01:05:19+00:002008-11-17T01:05:19+00:005e5eee28dd7870883785b8aad445c075dSolutionmthkhttp://biolab.isis.rl.ac.uk/uri/achttp://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.xml?revision=43243343320 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2O
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
Buffer pD 6.5 with Ca2+




This Post is Linked By: SANS on LOQ template; ]]>2008-11-17T01:05:45+00:002008-11-17T01:05:45+00:00509206e556e6792b92bdb7b6765a416d6Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/adhttp://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.xml?revision=433434438SANS of dialysed octameric RCK samples
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SANS
Instrument: LOQ
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Octameric RCK plus (no Ca) dialysed into pD 6.5 bufferT1046302
20 mM MES pD6.5 250 mM KCl in D2OT1046303
Octameric RCK plus Ca dialysed into pD6.5T1046304
20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2OT1046305
Octameric RCK plus (no Ca) dialysed into pD 6.5 bufferS8546306
20 mM MES pD6.5 250 mM KCl in D2OS8546307
Octameric RCK plus Ca dialysed into pD6.5S8546308
20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2OS8546309
Octameric RCK plus (no Ca) dialysed into pD 6.5 bufferS8546310
20 mM MES pD6.5 250 mM KCl in D2OS8546311
Octameric RCK plus Ca dialysed into pD6.5S8546312
20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2OS8546313

]]>2008-11-17T01:07:34+00:002008-11-17T01:14:29+00:005b4d0546b1766c45aea42e8762099d940SANSLOQhttp://biolab.isis.rl.ac.uk/data/92.xmlhttp://biolab.isis.rl.ac.uk/uri/aehttp://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml?revision=434http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml?revision=435http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml?revision=436http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml?revision=437http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml?revision=438374446Fresh beads for Laser Tweezers experiments
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Laser_tweezers_tus
We have done a bunch of fairly random preliminary experiments that suggest that the beads made have very different stickiness. The 1:100 gly-oh beads after ammonia treatment seem to be pretty non-sticky but after protein loading they become much more sticky. We are going to prepare protein loaded beads on good quantities of beads to split into different buffers and wash extensively.

Sortase reaction mixture made from:
50 uL Sortase (8.4 uM)
50 uL CaCl2 (100 mM)
368 uL Tus (62 uM)
32 uL water



BeadsWt of tubeWt of tube + beadsuL Sortase reactionProduct
1:100 gly-oh beads after ammonia treatment1.54821.5877100
1:1000 gly-OH beads after ammonia treatment1.64341.64861001;1000 Tus loaded silica beads
1:10000 gly-OH beads after ammonia treatment1.64311.6473100New 1:10,000 Tus labelled Silica beads
1:100,000 gly-OH beads after ammonia treatment1.59061.5942100


The beads were incubated overnight with shaking at room temperature. In the morning they were washed twice with 5 mM Tris-HCl pH 7.5, 1 M NaCL (1 mL), then 1 x 1 mL of the same buffer with 400 mM NaCl, then 1 x 1 mL of buffer with 300 mM NaCl. Then 1 x 1 mL of 100 mM NaCl buffer.

After resuspension in 100 mM NaCl buffer the samples were then split into 4 x 250 uL. The 250 uL samples were spun and resuspended in 1 mL of 100 mM NaCl buffer, 50 mM NaCl buffer, 0 mM NaCl buffer and water and washed a further two times with this buffer.
This Post is Linked By: New 1:10,000 Tus labelled Silica beads;1;1000 Tus loaded silica beads; ]]>2008-11-13T17:52:44+00:002008-11-27T13:24:08+00:0055a4f761c532ac99880cabdb3a8a580a9Laser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/99http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml?revision=374http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml?revision=376http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml?revision=378http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml?revision=381http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml?revision=446454454DNA sequence data from Southampton on pLLC064
Data
cameron.neylon.myopenid.comCameron NeylonData Type: Sequence_DNA
Sequence data forwarded by guillame boucher from sequencing of the plasmid pLLC064 - supposedly Sortase in pETMCSI

TTTTGTTTACTTTAAGAAGGAGATATACATATGAAACCACATATCGATAATTATCTTCAC
GATAAAGATAAAGATGAAAAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGT
AAAGATAAAAAGCAGCAAGCTAAACCTCAAATTCCGAAAGATAAATCGAAAGTGGCAGGC
TATATTGAAATTCCAGATGCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCT
GAACAATTAAATAGAGGTGTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAAT
ATTTCAATTGCAGGACACACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAA
GCAGCCAAAAAAGGTAGTATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAA
ATGACAAGTATAAGAGATGTTAAGCCTACAGATGTAGGAGTTCTAGATGAACAAAAAGGT
AAAGATAAACAATTAACATTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGG
GAAAAACGTAAAATCTTTGTAGCTACAGAAGTCAAATAATCGAATTCGAGCTCCCGGGTA
CCATGGCATGCATCGATAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT
GCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGG
GGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCACAGGACGGGTGTGGTCGCCAT
GATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTGGGCGGCGGCCAAA
GCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAAATTGCATCAACGCATATAGCGC
TAGCAGCACGCCATAGTGACTGGCCGATGCTGTCGGAATGGACGATATCCCGCAAGAGGC
CCGGCAGTACCGGCATAACCAAGCCTATGCCT
]]>2008-11-28T11:02:19+00:002008-11-28T11:02:19+00:0051d219eabdb43425aad7b512a551f1ab2Sequence_DNAhttp://biolab.isis.rl.ac.uk/uri/b7http://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.xml?revision=454474476Analysis of pLLC064 sequencing data
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Data_analysis
Looking at the sequence provided by Guillame and comparing it to the sequences of pETMCSI and pETMCSIII it is evident that this plasmid is the original sequence that Krystle cloned into pETMCSI in 2004 rather than the version is pETMCSIII. This explains why in previous work the protein didn't bind to the nickel column but was apparently well expressed. My guess is that Lilyan mis-labelled the plasmids when they were placed in the storage box. It also means that the plasmid I have sent out to various people isn't what I said it was.

The good news is that Ali Tavasolli's group at Soton are going to re-clone "our" Sortase gene and we can then re-make the properly designated pLLC064.
]]>2008-12-01T11:16:32+00:002008-12-01T11:19:11+00:0051a8ea5e0a6017a464a35d3b7b61f9639Data_analysishttp://biolab.isis.rl.ac.uk/uri/c1http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.xml?revision=474http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.xml?revision=475http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.xml?revision=476766766PCR template
Templates
cameron.neylon.myopenid.comCameron NeylonProcedure]] [[Procedure>PCR]]]]>


Reaction		TemplateuLPrimer 1ulPrimer 2uLBufferuLEnzymeuLdNTPulMguLWater (uL)Product

1		[[Dna:%]][[box=3]][[Dna:oligonucleotide]][[box=3]][[Dna:oligonucleotide]][[box=3]]
[[box]][[box=3]][[box]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]]
[[Dna:pcr_product]]

2		[[Dna:%]][[box=3]][[Dna:oligonucleotide]][[box=3]][[Dna:oligonucleotide]][[box=3]]
[[box]][[box=3]][[box]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]]
[[Dna:pcr_product]]

3		[[Dna:%]][[box=3]][[Dna:oligonucleotide]][[box=3]][[Dna:oligonucleotide]][[box=3]]
[[box]][[box=3]][[box]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]]
[[Dna:pcr_product]]

4		[[Dna:%]][[box=3]][[Dna:oligonucleotide]][[box=3]][[Dna:oligonucleotide]][[box=3]]
[[box]][[box=3]][[box]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]]
[[Dna:pcr_product]]

5		[[Dna:%]][[box=3]][[Dna:oligonucleotide]][[box=3]][[Dna:oligonucleotide]][[box=3]]
[[box]][[box=3]][[box]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]][[box=3]]
[[Dna:pcr_product]]


[[Section>Procedure]]
[[Procedure>PCR]]
]]>2008-12-09T10:36:01+00:002008-12-09T10:36:01+00:005d4e046ea08b8353327e4e4349245018fhttp://biolab.isis.rl.ac.uk/uri/110http://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.xml?revision=766390815Buffer background for octameric rck domain
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: mthk
20 mM Tris-HCl pD 8.0, 250 mM KCl
This Post is Linked By: SANS runs on octameric RCK;Additional runs on octameric Rck;Addition of Ca++ to octameric mthk sample; ]]>2008-11-16T11:23:39+00:002008-12-10T13:51:32+00:0052caddbf4274f8312722a7214fcb761b2Solutionmthkhttp://biolab.isis.rl.ac.uk/uri/9ehttp://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.xml?revision=390http://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.xml?revision=815765816KcsA plasmid from Southampton
Materials
cameron.neylon.myopenid.comCameron NeylonDna: plasmid
Material: Solution
A pET based plasmid containing the KcsA gene, a few micrograms resuspended in 20 uL of nuclease free water.
This Post is Linked By: Test PCR to insert Sortase sequence within KcsA; ]]>2008-12-09T10:34:18+00:002008-12-10T13:54:42+00:005f1474f2acae2bd3c829a5d5b2dddbb0aplasmidSolutionhttp://biolab.isis.rl.ac.uk/uri/10fhttp://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.xml?revision=765http://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.xml?revision=816757817kcsa-sort-bwd
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
Namekcsa-sort-bwd
Number
Location
SequenceCTGCCGGGTACCGGTGGTGCGCAGCTGATCACGTATCCGC
Length40
Melting temp71 (64) C
SupplierInvitrogen
Stock concentration100 uM

This Post is Linked By: Test PCR to insert Sortase sequence within KcsA; ]]>2008-12-09T10:24:45+00:002008-12-10T13:55:44+00:005ff7f3737c4f62cda5789e430513d3affoligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/116.xmlhttp://biolab.isis.rl.ac.uk/data/118.xmlhttp://biolab.isis.rl.ac.uk/uri/10ehttp://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml?revision=757http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml?revision=758http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml?revision=759http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml?revision=760http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml?revision=817756818kcsa-sort-fwd
Materials
cameron.neylon.myopenid.comCameron NeylonDna: oligonucleotide
Material: Solution
PropertyData
Namekcsa-sort-fwd
Number
Location
SequenceCGCACCACCGGTACCGGCAGACCTGCGCCGCCTCAGCCAG
Length40
Melting temp74 (64) C
SupplierInvitrogen
Stock concentration100 uM

This Post is Linked By: Test PCR to insert Sortase sequence within KcsA; ]]>2008-12-09T10:23:18+00:002008-12-10T13:55:59+00:00520ac5712f22bb4333175022ae283d31eoligonucleotideSolutionhttp://biolab.isis.rl.ac.uk/data/120.xmlhttp://biolab.isis.rl.ac.uk/data/122.xmlhttp://biolab.isis.rl.ac.uk/uri/10dhttp://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml?revision=756http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml?revision=761http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml?revision=762http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml?revision=763http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml?revision=818769820Test PCR to insert Sortase sequence within KcsA
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: PCR
Project: kcsa
Two large PCR reactions masters were set up as described in the table.



Reaction		TemplateuLPrimer 1ulPrimer 2uLBufferuLEnzymeuLdNTPulMguLWater (uL)Product

1		KcsA plasmid from Southampton5kcsa-sort-fwd1kcsa-sort-bwd1
Thermopol buffer10Vent12 mM10100 mM072

2		KcsA plasmid from Southampton5kcsa-sort-fwd1kcsa-sort-bwd1
Thermopol buffer10Vent12 mM10100 mM171


Each of the two was then divided into ten PCR tubes and spread across the plate of the thermal cycler to have a gradient on the annealing phase of the PCR reaction from 40-60 C.

Samples of each rxn (5 uL) were run on an agarose gel and stained. Not a thing on it! Which is odd given the amount of MgSO4 in the second set of reactions. Will have to try again...
]]>2008-12-09T10:47:35+00:002008-12-10T14:16:59+00:00558aa3a920bb1641d5a80e924993eda5dPCRkcsahttp://biolab.isis.rl.ac.uk/data/124.xmlhttp://biolab.isis.rl.ac.uk/uri/112http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=769http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=772http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=773http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=774http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=775http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=776http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=819http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml?revision=8203532261First preparation of beads for laser tweezers experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Laser_tweezers_tus
Approximately 1 mg of 1:100 gly-oh beads after ammonia treatment was taken for resuspension in Sortase labelling reaction. Phosphorylated TerR lam (1uL) was added to Bangs Labs streptavidin coated beads (10uL) and allowed to incubate for ~15 minutes after which 1 mL of water was added and the beads spun, supernatant discarded and the beads resuspended in 1mL of water.
]]>2008-11-12T14:12:44+00:002009-01-23T15:03:27+00:0059f8189d2d6d1e3421fb30f3166051a68Laser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/90http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.xml?revision=353http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.xml?revision=22612612262Phosphorylation of DNA substrates for laser tweezers experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: DNA_phosphorylation
Project: Laser_tweezers_tus

DNA substratevolumeT4 Polynucleotide Kinase5 x Kinase Forward reaction bufferATP (10 mM)WaterProduct
Ethanol precipitated lambda DNA200150250Phosphorylated lambda DNA
Oligo-lambda-biotin10152.57.5Phosphorylated oligo-lambda-biotin
Oligo-TerRlam10152.57.5Phosphorylated TerR lam
Oligo-TerR10152.57.5Phosporylated TerR
Oligo-TerFlam10152.57.5Phosphorylated TerRlam
Oligo-TerF10152.57.5Phosphorylated TerR


Samples were prepared as given and incubated at 37 C for 10' followed by 10' at 65 C to inactivate the enzyme.
This Post is Linked By: Phosphorylated TerR lam;Phosporylated TerR;Phosphorylated TerRlam;Phosphorylated oligo-lambda-biotin;Phosphorylated lambda DNA;Phosphorylated TerR; ]]>2008-11-05T15:59:58+00:002009-01-23T15:03:53+00:005e9a9f853ecf07850581e7ab794784a6cDNA_phosphorylationLaser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/65http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xml?revision=261http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xml?revision=270http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xml?revision=274http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xml?revision=22622392263Ethanol precipitation of lambda DNA
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Laser_tweezers_tus
Because the Lambda DNA is in a tris-edta buffer I will ethanol ppt it and resuspend in water.

200 uL of Lambda DNA was added to 20 uL of 3 M ammonium acetate pH 4.8 followed by 400 uL of ethanol. The solution was mixed and incubated on ice for ~60 minutes. The solution was then centrifuged at 20k x g for 20 minutes.

The DNA was then further dried overnight and resuspended in nuclease free water to give Ethanol precipitated lambda DNA
This Post is Linked By: Ethanol precipitated lambda DNA; ]]>2008-10-28T15:35:06+00:002009-01-23T15:04:27+00:005d4a62a6806f5e7bb7c0317df6f16bd3eLaser_tweezers_tushttp://biolab.isis.rl.ac.uk/uri/5bhttp://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xml?revision=239http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xml?revision=241http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xml?revision=243http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xml?revision=226328683262Further lysozyme runs
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate. 0.5 mm of Al was used to attenuate the beam from this point on.


SampleFramesExposure time I22 Raw Run #Data
Lysozyme (~10 mg/mL)3030A10000.202Lysozyme 10 mg/mL with attenutor
Lysozyme (~20 mg/mL)3030A11000.202Lysozyme 20 mg/mL w/o attenutor
GFP (~2 mg/mL)3030A12000.2020GFP 2mg/mL
GFP (~2 mg/mL)1030A13000.202second 10 frames of 2 mg/mL GFP
GFP (~5 mg/mL)3030A14000.202GFP 5 mg/mL
GFP (~10mg/mL)3030A15000.202GFP 10mg/mL
GFP (~20 mg/mL)3030A16000.202GFP 20mg/mL

]]>2009-02-02T19:25:10+00:002009-02-10T11:36:21+00:00591ffa4469e7428b450cc167ead31edf0SAXSI22http://biolab.isis.rl.ac.uk/uri/16dhttp://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=3262http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=2868http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=2869http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=2870http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=2871http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml?revision=288932263228UV-Vis of 5mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
FromUV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:33:38+00:002009-02-09T15:33:54+00:00588ab82378cc2663c932d9ccbc0b06b3fUV-Vishttp://biolab.isis.rl.ac.uk/data/382.xmlhttp://biolab.isis.rl.ac.uk/uri/1a9http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.xml?revision=3228http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.xml?revision=3226http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.xml?revision=322732293229UV-Vis of 2mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS
This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:34:28+00:002009-02-09T15:34:28+00:005a156ec6c8397903f734509413c2cba95UV-Vishttp://biolab.isis.rl.ac.uk/uri/1aahttp://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.xml?revision=322932303232UV-Vis of 20mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:34:55+00:002009-02-09T15:35:11+00:00591b41b2b6d3c8ee782f51c8b659ec0baUV-Vishttp://biolab.isis.rl.ac.uk/data/384.xmlhttp://biolab.isis.rl.ac.uk/uri/1abhttp://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.xml?revision=3230http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.xml?revision=3231http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.xml?revision=323232333235UV-Vis of 10mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:35:35+00:002009-02-09T15:36:03+00:005fe7d37bb8a4bd18e0ef4b62ec265928aUV-Vishttp://biolab.isis.rl.ac.uk/data/386.xmlhttp://biolab.isis.rl.ac.uk/uri/1achttp://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.xml?revision=3233http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.xml?revision=3234http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.xml?revision=323532363238UV-Vis of 5mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:36:27+00:002009-02-09T15:36:45+00:005b1a9b6feb83441f5a2d8adc87fd2a569UV-Vishttp://biolab.isis.rl.ac.uk/data/388.xmlhttp://biolab.isis.rl.ac.uk/uri/1adhttp://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.xml?revision=3236http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.xml?revision=3237http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.xml?revision=323832393241UV-Vis of 2mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:37:09+00:002009-02-09T15:37:24+00:005cb7457c3feec927f51ee2b6380cdadf1UV-Vishttp://biolab.isis.rl.ac.uk/data/390.xmlhttp://biolab.isis.rl.ac.uk/uri/1aehttp://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.xml?revision=3239http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.xml?revision=3240http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.xml?revision=324132193242UV-Vis of Lysozyme and GFP samples from I22 SAXS
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: UV-Vis
Samples (10 uL) were run in a 5mm path length microcell on the GeneQuant 1300. The instrument was zeroed with a water background.


SampleDilutionData
Lysozyme (~20 mg/mL)20-foldUV-Vis of 20mg/mL Lysozyme
Lysozyme (~10 mg/mL)20-foldUV-Vis of 10mg/mL Lysozyme
Lysozyme (~5 mg/mL)10-foldUV-Vis of 5mg/mL Lysozyme
Lysozyme (~2 mg/mL)5-foldUV-Vis of 2mg/mL Lysozyme
GFP (~20 mg/mL)10-foldUV-Vis of 20mg/mL GFP
GFP (~10mg/mL)2-foldUV-Vis of 10mg/mL GFP
GFP (~5 mg/mL)2-foldUV-Vis of 5mg/mL GFP
GFP (~2 mg/mL)N/aUV-Vis of 2mg/mL GFP

This Post is Linked By: UV-Vis of 5mg/mL Lysozyme;UV-Vis of 2mg/mL Lysozyme;UV-Vis of 20mg/mL GFP;UV-Vis of 10mg/mL GFP;UV-Vis of 5mg/mL GFP;UV-Vis of 2mg/mL GFP;UV-Vis of 20mg/mL Lysozyme;UV-Vis of 10mg/mL Lysozyme; ]]>2009-02-09T14:45:03+00:002009-02-09T15:38:25+00:005de3c0448e4c1fe295991582a625a8acfUV-Vishttp://biolab.isis.rl.ac.uk/uri/1a6http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.xml?revision=3242http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.xml?revision=321932203222UV-Vis of 20mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:32:00+00:002009-02-09T15:32:26+00:005ebcdde308ba7f83cf0cd68536076dc00UV-Vishttp://biolab.isis.rl.ac.uk/data/378.xmlhttp://biolab.isis.rl.ac.uk/uri/1a7http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.xml?revision=3220http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.xml?revision=3221http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.xml?revision=322232233225UV-Vis of 10mg/mL Lysozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: UV-Vis
From UV-Vis of Lysozyme and GFP samples from I22 SAXS

This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS; ]]>2009-02-09T15:32:51+00:002009-02-09T15:33:09+00:0054a7b4800847304ae2d263ea4cf6797b9UV-Vishttp://biolab.isis.rl.ac.uk/data/380.xmlhttp://biolab.isis.rl.ac.uk/uri/1a8http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.xml?revision=3223http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.xml?revision=3224http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.xml?revision=322527652765Preparation of DMPC vesicles
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Reaction_centre
DMPC (20.6 mg) was dissolved in 10 mL of chloroform and transferred to a conical flask. The chloroform was evaporated under a stream of dry nitrogen.
]]>2009-02-01T12:48:21+00:002009-02-01T12:48:21+00:0055ec7dc98f5b23c7438ebcbb3f844376eReaction_centrehttp://biolab.isis.rl.ac.uk/uri/14bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.xml?revision=276527742775Exchange of prBC into bOG
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Reaction_centre
Following the procedure from http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/5591 for detergent exchange.

Stock solution of 20 mM Tris-HCl pH 8 is available in the lab. Will use this as the base for buffers.

beta octyl glucoside (0.4104 g) was dissolved in 70 mL of Tris buffer.

Approx 1.5 mL of prBC sample was loaded onto a DEAE-FF column equilibrated in Tris buffer. The column was loaded and then washed with ~50 mL of buffer. The column was then exchanged into Tris buffer with 20 mM beta octyl glucoside and the protein attempted to be eluted with Tris buffer with beta octyl glucoside plus 200 mM NaCl.

Took four fractions and did UV absorbance but no significant peak at ~800 expected for prBC. Also no visible purple throughout run which suggests protein did not bind to column.
]]>2009-02-01T15:00:06+00:002009-02-01T16:00:51+00:0057900c0c44ee9d7bb38908a62e9286d3cReaction_centrehttp://biolab.isis.rl.ac.uk/uri/14ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.xml?revision=2774http://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.xml?revision=277527782780Lysozyme (~20 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Solution of lysozyme at approx 20 mg/mL from Preparation of lysozyme solutions
This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;SAXS on I22 template;Preparation of lysozyme solutions; ]]>2009-02-01T16:30:33+00:002009-02-01T16:31:00+00:005d74dae5f57c53383af1c46830b4a7c4aSolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/151http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.xml?revision=2778http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.xml?revision=278027822785GFP (~20 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonProject: SAS_standard
Approx 20 mg/mL GFP prepared in Preparation of GFP solutions for I22 SAXS
This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;Preparation of GFP solutions for I22 SAXS; ]]>2009-02-01T16:32:27+00:002009-02-01T16:57:28+00:005f5bf61d81d0fc1b347129080e130947aSAS_standardhttp://biolab.isis.rl.ac.uk/uri/153http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.xml?revision=2782http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.xml?revision=278527862786GFP (~10mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 10 mg/mL GFP prepared in Preparation of GFP solutions for I22 SAXS




This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;Preparation of GFP solutions for I22 SAXS; ]]>2009-02-01T16:57:42+00:002009-02-01T16:57:42+00:005a63ccd63accd70b1f7208c9834237f86SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/155http://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.xml?revision=278627872787GFP (~5 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 5 mg/mL GFP prepared in Preparation of GFP solutions for I22 SAXS




This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;Preparation of GFP solutions for I22 SAXS; ]]>2009-02-01T16:58:14+00:002009-02-01T16:58:14+00:0054840dc712155fa25b8322d7238dacad1SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/156http://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.xml?revision=278727882788GFP (~2 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 2 mg/mL GFP prepared in Preparation of GFP solutions for I22 SAXS




This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;Preparation of GFP solutions for I22 SAXS; ]]>2009-02-01T16:58:42+00:002009-02-01T16:58:42+00:00578b052f22d66cf8616c4f6bb66be34eaSolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/157http://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.xml?revision=278827902790Lysozyme (~10 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 10 mg/mL lysozyme prepared in Preparation of lysozyme solutions




This Post is Linked By: Further lysozyme runs;UV-Vis of Lysozyme and GFP samples from I22 SAXS;Preparation of lysozyme solutions; ]]>2009-02-01T17:01:00+00:002009-02-01T17:01:00+00:0055a6577b6f4e1354c55972f5b346d96f6SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/158http://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.xml?revision=279027912791Lysozyme (~5 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 5 mg/mL lysozyme prepared in Preparation of lysozyme solutions




This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS;SAXS on I22 template;Preparation of lysozyme solutions; ]]>2009-02-01T17:01:26+00:002009-02-01T17:01:26+00:005b95c8b6aec4c44624d72a299a4095ac1SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/159http://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.xml?revision=279127922792Lysozyme (~2 mg/mL)
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
Approx 2 mg/mL lysozyme prepared in Preparation of lysozyme solutions




This Post is Linked By: UV-Vis of Lysozyme and GFP samples from I22 SAXS;SAXS on I22 template;Preparation of lysozyme solutions; ]]>2009-02-01T17:01:52+00:002009-02-01T17:01:52+00:0053390efdf4bdc11c29c4bd74985a75f27SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/15ahttp://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.xml?revision=279228162819Tris-HCl pH 8.0 buffer
Materials
cameron.neylon.myopenid.comCameron NeylonProject: SAS_standard
Material: Solution
tris-hcl pH 8.0 buffer
This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T14:16:52+00:002009-02-02T14:20:16+00:005e49ed9eeb95366c0c5f79c0e8ea1d898SAS_standardSolutionhttp://biolab.isis.rl.ac.uk/uri/15bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.xml?revision=2816http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.xml?revision=2817http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.xml?revision=281928222822SAXS on Tris-HCl pH 8.0 buffer
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A02001.202A02000.DAT





This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T14:30:45+00:002009-02-02T14:30:45+00:00543ebe9b1ac30baa256f06d098583cccbSAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/uri/15ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.xml?revision=282228382842Lysozyme 10 mg/mL with attenutor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A10000.202A10000.DAT



This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T17:22:34+00:002009-02-02T17:26:28+00:005394326e79f09d27e1f2f8646937f8569SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/314.xmlhttp://biolab.isis.rl.ac.uk/uri/167http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.xml?revision=2838http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.xml?revision=2841http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.xml?revision=284228342846Lysozyme 5mg/mL with attenuator
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A09000.202A09000.DAT



This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T16:50:50+00:002009-02-02T17:27:18+00:005c71628d0624d2f869fae8e60705f54e7SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/318.xmlhttp://biolab.isis.rl.ac.uk/uri/164http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.xml?revision=2834http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.xml?revision=2845http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.xml?revision=284628352847Lysozyme 2mg/mL with attenuator
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A08000.202A08000.DAT

This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T16:51:59+00:002009-02-02T17:27:32+00:0052202e93ef954831358a71d408d1bf4aeSAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/316.xmlhttp://biolab.isis.rl.ac.uk/uri/165http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xml?revision=2835http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xml?revision=2843http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xml?revision=2844http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xml?revision=284728332849Buffer with attentuator
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A07000.202A07000.DAT



This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T16:50:11+00:002009-02-02T17:28:05+00:005a4018a418fca531afe19bb3085eb53a7SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/320.xmlhttp://biolab.isis.rl.ac.uk/uri/163http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.xml?revision=2833http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.xml?revision=2848http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.xml?revision=284928322851Lysozyme 20 mg/mL w/o attenutor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A05000.202A05000.DAT



]]>2009-02-02T16:49:20+00:002009-02-02T17:32:45+00:00519ada060b585476abfd99ee49d9f3321SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/322.xmlhttp://biolab.isis.rl.ac.uk/uri/162http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.xml?revision=2832http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.xml?revision=2850http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.xml?revision=285128312853Lysozyme 5mg/mL w/o attenuator
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A04000.202A04000.DAT



]]>2009-02-02T16:47:48+00:002009-02-02T17:33:14+00:0051e8e7aa545f63a1d7eb796ab1593b724SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/324.xmlhttp://biolab.isis.rl.ac.uk/uri/161http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.xml?revision=2831http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.xml?revision=2852http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.xml?revision=285328242855First run on 2 mg/ml Lyzozyme
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A03000.202A03000.DAT



This Post is Linked By: SAXS on I22 template; ]]>2009-02-02T15:56:56+00:002009-02-02T17:33:40+00:005553fb24951904a864a62cb4b3ee99bcbSAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/326.xmlhttp://biolab.isis.rl.ac.uk/uri/15fhttp://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xml?revision=2824http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xml?revision=2828http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xml?revision=2854http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xml?revision=285528372857Lysozyme 20 mg/mL with attenutor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A11000.202A11000.DAT



This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T17:22:01+00:002009-02-02T17:41:04+00:00539844854a67d01844e5328ac8b3efd18SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/328.xmlhttp://biolab.isis.rl.ac.uk/uri/166http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xml?revision=2837http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xml?revision=2839http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xml?revision=2856http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xml?revision=285728582858GFP 2mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A12000.202A12001.DAT






This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T17:44:08+00:002009-02-02T17:44:08+00:005fab53c0d6dd43f8255c9c1b14c294472SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/uri/168http://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.xml?revision=285828592862second 10 frames of 2 mg/mL GFP
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A13000.202A13001.DAT



This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T18:30:15+00:002009-02-02T18:31:31+00:0056421cb054135fcae985648d16ee9801aSAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/330.xmlhttp://biolab.isis.rl.ac.uk/uri/169http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.xml?revision=2859http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.xml?revision=2861http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.xml?revision=286228602867GFP 5 mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A14000.202A14001.202



This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T18:30:48+00:002009-02-02T18:54:11+00:00515817596e54a184d8c8d5addc34a2977SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/332.xmlhttp://biolab.isis.rl.ac.uk/uri/16ahttp://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.xml?revision=2860http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.xml?revision=2866http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.xml?revision=2867287228730.25 mg/mL GluR0 with glutamate
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
0.25 mg/mL GluR0 with glutamate prepared by Michael
This Post is Linked By: SAXS runs of GluR0; ]]>2009-02-02T19:28:39+00:002009-02-02T19:28:54+00:0052447829cf1671de53d9ad843ddec4958SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/16ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.xml?revision=2872http://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.xml?revision=2873287428750.5 mg/mL GluR0 with glutamate
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SAS_standard
0.5 mg/mL GluR0 with glutamate prepared by Michael
This Post is Linked By: SAXS runs of GluR0; ]]>2009-02-02T19:29:27+00:002009-02-02T19:29:40+00:00530185546126fce4f82fc60cbcdb63169SolutionSAS_standardhttp://biolab.isis.rl.ac.uk/uri/16fhttp://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.xml?revision=2874http://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.xml?revision=287528632878GFP 10mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A15000.202A15001.DAT

This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T18:37:12+00:002009-02-02T19:36:27+00:0051fc6a8cbc2fa410ef85f848c319d6cc6SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/334.xmlhttp://biolab.isis.rl.ac.uk/uri/16bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.xml?revision=2863http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.xml?revision=2877http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.xml?revision=287828642880GFP 20mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: SAS_standard

Raw data fileData file
A16000.202A16001.DAT

This Post is Linked By: Further lysozyme runs; ]]>2009-02-02T18:37:38+00:002009-02-02T19:36:54+00:005b4f6fdd92687ab2aea0e3a97ca61c9d0SAXS_DiamondI22SAS_standardhttp://biolab.isis.rl.ac.uk/data/336.xmlhttp://biolab.isis.rl.ac.uk/uri/16chttp://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.xml?revision=2864http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.xml?revision=2879http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.xml?revision=288028252890SAXS on I22 template
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
Tris-HCl pH 8.0 buffer110A2001.202SAXS on Tris-HCl pH 8.0 buffer
Lysozyme (~2 mg/mL)1010A03000.202First run on 20 mg/ml Lyzozyme
Lysozyme (~5 mg/mL)1010A04000.202
Lysozyme (~20 mg/mL)1010A05000.202
Tris-HCl pH 8.0 buffer1010A06000.202
Tris-HCl pH 8.0 buffer3030A07000.202Buffer with attentuator
Lysozyme (~2 mg/mL)3030A08000.202Lysozyme 2mg/mL with attenuator
Lysozyme (~5 mg/mL)3030A09000.202Lysozyme 5mg/mL with attenuator


After first four runs put in attentuator (10x, 0.5 mm of Al)
]]>2009-02-02T16:22:56+00:002009-02-02T22:46:49+00:005a5964748f42db06e1f8ba9e3084b6c58SAXSI22http://biolab.isis.rl.ac.uk/uri/160http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=2825http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=2826http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=2827http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=2830http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=2836http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml?revision=289028962897GluR0 buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: GluR0
GluR0 buffer prepared by Michael
This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-02T23:00:41+00:002009-02-02T23:00:53+00:005992e6867cafaa0d8649329bcea09c290SolutionGluR0http://biolab.isis.rl.ac.uk/uri/173http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.xml?revision=2896http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.xml?revision=289728862899SAXS runs of GluR0
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
Project: GluR0
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
0.25 mg/mL GluR0 with glutamate3030A17000.202GluR0 SAXS 0.25 mg/mL + glu first run
0.5 mg/mL GluR0 with glutamate3030A1800.202SAXS of 0.5 mg/mL GluR0 with glutamate


SAXS from 0.5 mg/mL seems poor compared to 0.25 mg/mL. Are these the wrong way around?
]]>2009-02-02T22:45:48+00:002009-02-02T23:02:27+00:005eb821b34fa198904c8eba01e48ad41a9SAXSI22GluR0http://biolab.isis.rl.ac.uk/uri/172http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml?revision=2886http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml?revision=2887http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml?revision=2888http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml?revision=2891http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml?revision=289929002901GluR0 1mg/mL no glutamate
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: GluR0
GluR0 1mg/mL no glutamate prepared by Michael
This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-02T23:11:51+00:002009-02-02T23:12:00+00:005734b6b262e779565e1f7fe69840bfdc1SolutionGluR0http://biolab.isis.rl.ac.uk/uri/174http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.xml?revision=2900http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.xml?revision=2901290329030.5 mg/mL GluR0 no glutamate
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: GluR0
0.5 mg/mL GluR0 no glutamate prepared by Michael




This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-02T23:12:36+00:002009-02-02T23:12:36+00:0054568c895e57bb3ba9a7dd42c8b70b69fSolutionGluR0http://biolab.isis.rl.ac.uk/uri/175http://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.xml?revision=2903290429040.25 mg/mL GluR0 no glutamate
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: GluR0
0.25 mg/mL GluR0 no glutamate prepared by Michael




This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-02T23:12:54+00:002009-02-02T23:12:54+00:005b6ce106577103b1f20f598c56b090f8fSolutionGluR0http://biolab.isis.rl.ac.uk/uri/176http://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.xml?revision=290428812906SAXS of 0.5 mg/mL GluR0 with glutamate
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A18000.202A18001.DAT

This Post is Linked By: SAXS runs of GluR0; ]]>2009-02-02T19:51:10+00:002009-02-02T23:14:13+00:005adcfa3381f32ae7bb8b5958ab1a47236SAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/338.xmlhttp://biolab.isis.rl.ac.uk/uri/171http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xml?revision=2881http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xml?revision=2892http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xml?revision=2905http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xml?revision=290628762908GluR0 SAXS 0.25 mg/mL + glu first run
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A17000.202A17001.DAT

This Post is Linked By: SAXS runs of GluR0; ]]>2009-02-02T19:34:56+00:002009-02-02T23:14:47+00:0058f07f1b876b459e529b5a7ab67a4366aSAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/340.xmlhttp://biolab.isis.rl.ac.uk/uri/170http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xml?revision=2876http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xml?revision=2893http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xml?revision=2907http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xml?revision=290829092911SAXS of GLuR0 buffer
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A19000.202A19001.DAT
A19001.DAT

This Post is Linked By: SAXS on GluR) in capillaries;Reprocessing SAXS data from Feb 09; ]]>2009-02-02T23:29:56+00:002009-02-02T23:30:16+00:005a3c7703c2a2e3cc3e6a1bc2047443521SAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/342.xmlhttp://biolab.isis.rl.ac.uk/uri/177http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.xml?revision=2909http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.xml?revision=2910http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.xml?revision=291129122914SAXS of 1 mg/mL GluR0 no glutamate
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A20000.202A20001.DAT
A20001.DAT

This Post is Linked By: SAXS on GluR) in capillaries;Reprocessing SAXS data from Feb 09; ]]>2009-02-02T23:31:15+00:002009-02-02T23:39:03+00:00520b44c25f7935af50c6571a7b5f40445SAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/344.xmlhttp://biolab.isis.rl.ac.uk/uri/178http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.xml?revision=2912http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.xml?revision=2913http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.xml?revision=291429152919SAXS of GluR0 0.5 mg/mL no glutamate
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A21000.202A21001.DAT

This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-02T23:48:42+00:002009-02-02T23:58:30+00:005f2e56a1bd2b5f44bb846dc75170dc51eSAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/346.xmlhttp://biolab.isis.rl.ac.uk/uri/179http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.xml?revision=2915http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.xml?revision=2918http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.xml?revision=291929212922SAXS on GluR) in capillaries
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
GluR0 buffer3030A19000.202SAXS of GLuR0 buffer
GluR0 1mg/mL no glutamate3030A20000.202SAXS of 1 mg/mL GluR0 no glutamate
0.5 mg/mL GluR0 no glutamate3030A21000.202SAXS of GluR0 0.5 mg/mL no glutamate
0.25 mg/mL GluR0 no glutamate303022000.202SAXS of GluR0 0.25 mg/mL no glutamate

]]>2009-02-03T00:12:57+00:002009-02-03T00:13:10+00:0055b7522d772e34d4d8d3dea009e843a5bSAXSI22http://biolab.isis.rl.ac.uk/uri/17bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.xml?revision=2921http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.xml?revision=292229202924SAXS of GluR0 0.25 mg/mL no glutamate
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A22000.202A22001.DAT

This Post is Linked By: SAXS on GluR) in capillaries; ]]>2009-02-03T00:04:04+00:002009-02-03T00:20:36+00:005a2eff19bd4647c55f2481b767d965a42SAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/348.xmlhttp://biolab.isis.rl.ac.uk/uri/17ahttp://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.xml?revision=2920http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.xml?revision=2923http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.xml?revision=292429262926Tus buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Tus_labelling
Tus buffer prepared by Shubbie




This Post is Linked By: SAXS of Tus samples; ]]>2009-02-03T00:24:09+00:002009-02-03T00:24:09+00:005d94bb89074e353205170b50eb6d08bf5SolutionTus_labellinghttp://biolab.isis.rl.ac.uk/uri/17chttp://biolab.isis.rl.ac.uk/camerons_labblog/2926/Tus_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2926/Tus_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2926/Tus_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2926/Tus_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2926/Tus_buffer.xml?revision=292629272927Tus unlabelled
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Tus_labelling
Unlabelled Tus prepared by Shubbie




This Post is Linked By: SAXS of Tus samples; ]]>2009-02-03T00:24:27+00:002009-02-03T00:24:27+00:00564ae4d9adb8cf347971ed92e86243ea0SolutionTus_labellinghttp://biolab.isis.rl.ac.uk/uri/17dhttp://biolab.isis.rl.ac.uk/camerons_labblog/2927/Tus_unlabelled.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2927/Tus_unlabelled.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2927/Tus_unlabelled.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2927/Tus_unlabelled.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2927/Tus_unlabelled.xml?revision=292729282928Fluorescein labelled Tus
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Tus_labelling
Fluorescein labelled Tus prepared by Shubbie




]]>2009-02-03T00:25:04+00:002009-02-03T00:25:04+00:00563e18f4ad73be938940fa260c73fd52fSolutionTus_labellinghttp://biolab.isis.rl.ac.uk/uri/17ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2928/Fluorescein_labelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2928/Fluorescein_labelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2928/Fluorescein_labelled_Tus.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2928/Fluorescein_labelled_Tus.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2928/Fluorescein_labelled_Tus.xml?revision=292829312931SAXS of unlabelled Tus
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Tus_labelling

Raw data fileData file
A24000.202A24001.DAT






This Post is Linked By: SAXS of Tus samples; ]]>2009-02-03T00:28:04+00:002009-02-03T00:28:04+00:005573d40b17bd0d526fa8bef45d9d3258bSAXS_DiamondI22Tus_labellinghttp://biolab.isis.rl.ac.uk/uri/180http://biolab.isis.rl.ac.uk/camerons_labblog/2931/SAXS_of_unlabelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2931/SAXS_of_unlabelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2931/SAXS_of_unlabelled_Tus.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2931/SAXS_of_unlabelled_Tus.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2931/SAXS_of_unlabelled_Tus.xml?revision=293129322932SAXS of labelled Tus
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Tus_labelling

Raw data fileData file
A25000.202A25001.DAT






This Post is Linked By: SAXS of Tus samples; ]]>2009-02-03T00:28:28+00:002009-02-03T00:28:28+00:005f3740e8cdcd2c8991930fa1935ce4975SAXS_DiamondI22Tus_labellinghttp://biolab.isis.rl.ac.uk/uri/181http://biolab.isis.rl.ac.uk/camerons_labblog/2932/SAXS_of_labelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2932/SAXS_of_labelled_Tus.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2932/SAXS_of_labelled_Tus.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2932/SAXS_of_labelled_Tus.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2932/SAXS_of_labelled_Tus.xml?revision=293229302936SAXS of Tus buffer
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Tus_labelling

Raw data fileData file
A23000.202A23000.DAT



This Post is Linked By: SAXS of Tus samples; ]]>2009-02-03T00:27:36+00:002009-02-03T00:36:28+00:00500194192f961bff0b356fb762610c965SAXS_DiamondI22Tus_labellinghttp://biolab.isis.rl.ac.uk/data/350.xmlhttp://biolab.isis.rl.ac.uk/data/352.xmlhttp://biolab.isis.rl.ac.uk/uri/17fhttp://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xml?revision=2930http://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xml?revision=2933http://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xml?revision=2934http://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xml?revision=2935http://biolab.isis.rl.ac.uk/camerons_labblog/2930/SAXS_of_Tus_buffer.xml?revision=293629372938SAXS of Tus samples
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
Tus buffer3030A23000.202SAXS of Tus buffer
Tus buffer3030A24000.202SAXS of unlabelled Tus
Tus unlabelled1090A25000.202SAXS of labelled Tus


No apparent scattering from Tus samples?!? even though supposed to be 2 mg/mL
]]>2009-02-03T01:12:24+00:002009-02-03T01:12:57+00:00530c376e9de4c49d5b8966c8b80dd98e7SAXSI22http://biolab.isis.rl.ac.uk/uri/182http://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.xml?revision=2937http://biolab.isis.rl.ac.uk/camerons_labblog/2937/SAXS_of_Tus_samples.xml?revision=293829402940JHL sample
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL Sample (23 mg/mL)




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T01:14:00+00:002009-02-03T01:14:00+00:005658445bf7cd756fbaa283358340e3a16SolutionJHLhttp://biolab.isis.rl.ac.uk/uri/183http://biolab.isis.rl.ac.uk/camerons_labblog/2940/JHL_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2940/JHL_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2940/JHL_sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2940/JHL_sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2940/JHL_sample.xml?revision=294029412941JHL buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL buffer




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T01:14:28+00:002009-02-03T01:14:28+00:0052fa77da867b321bed7aa763fe1582924SolutionJHLhttp://biolab.isis.rl.ac.uk/uri/184http://biolab.isis.rl.ac.uk/camerons_labblog/2941/JHL_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2941/JHL_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2941/JHL_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2941/JHL_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2941/JHL_buffer.xml?revision=294129422942JHL 10mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL 10 mg/mL




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T01:50:29+00:002009-02-03T01:50:29+00:005268f9c2e46001ba380ed333c71611867SolutionJHLhttp://biolab.isis.rl.ac.uk/uri/185http://biolab.isis.rl.ac.uk/camerons_labblog/2942/JHL_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2942/JHL_10mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2942/JHL_10mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2942/JHL_10mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2942/JHL_10mgmL.xml?revision=294229442944JHL RP 5 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL RP 5 mg/mL




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T02:09:23+00:002009-02-03T02:09:23+00:0054f4dab9f5d6b768cb819d88c62fe04d6SolutionJHLhttp://biolab.isis.rl.ac.uk/uri/187http://biolab.isis.rl.ac.uk/camerons_labblog/2944/JHL_RP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2944/JHL_RP_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2944/JHL_RP_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2944/JHL_RP_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2944/JHL_RP_5_mgmL.xml?revision=294429452945JHL N 10 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL N 10 mg/mL




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T02:15:30+00:002009-02-03T02:15:30+00:0058d58fd47d86ebaf916f1c6d6c18f756dSolutionJHLhttp://biolab.isis.rl.ac.uk/uri/188http://biolab.isis.rl.ac.uk/camerons_labblog/2945/JHL_N_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2945/JHL_N_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2945/JHL_N_10_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2945/JHL_N_10_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2945/JHL_N_10_mgmL.xml?revision=294529462946JHL N 5 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: JHL
JHL N 5 mg/mL




This Post is Linked By: SAXS for JHL; ]]>2009-02-03T02:24:55+00:002009-02-03T02:24:55+00:005152ffe03406e539dd97f4cd245e72a0eSolutionJHLhttp://biolab.isis.rl.ac.uk/uri/189http://biolab.isis.rl.ac.uk/camerons_labblog/2946/JHL_N_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2946/JHL_N_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2946/JHL_N_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2946/JHL_N_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2946/JHL_N_5_mgmL.xml?revision=294629432947SAXS for JHL
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
JHL sample1090A26000.202
JHL buffer1090A27000.202
JHL 10mg/mL1090A28000.202
JHL RP 5 mg/mL2-390A29000.203
JHL N 10 mg/mL1090A30000.203
JHL N 5 mg/mL1090A31000.203

]]>2009-02-03T01:50:36+00:002009-02-03T02:25:31+00:0051a732a5baea4ce3ddb365f05c60784e6SAXSI22http://biolab.isis.rl.ac.uk/uri/186http://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.xml?revision=2943http://biolab.isis.rl.ac.uk/camerons_labblog/2943/SAXS_for_JHL.xml?revision=294729482948ATIC - Buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
ATIC buffer from Southampton




This Post is Linked By: SAXS on ATIC;Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T02:43:21+00:002009-02-03T02:43:21+00:00537d6893f93b48378338a4ca7edc01101SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/18ahttp://biolab.isis.rl.ac.uk/camerons_labblog/2948/ATIC__Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2948/ATIC__Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2948/ATIC__Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2948/ATIC__Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2948/ATIC__Buffer.xml?revision=294829492949ATIC 20 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
ATIC 20 mg/mL




This Post is Linked By: SAXS on ATIC;Addition of inhibitor to ATIC solutions;Further preparation of samples with inhibitor;Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T02:43:46+00:002009-02-03T02:43:46+00:005f1efcbaececbcf585ec4e193fd7ec840SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/18bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2949/ATIC_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2949/ATIC_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2949/ATIC_20_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2949/ATIC_20_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2949/ATIC_20_mgmL.xml?revision=294929502950ATIC 10 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
ATIC 10 mg/mL




This Post is Linked By: SAXS on ATIC;Addition of inhibitor to ATIC solutions;Further preparation of samples with inhibitor; ]]>2009-02-03T02:44:10+00:002009-02-03T02:44:10+00:005197e3a8a4b48be6902b4ec5d0a1fdcc6SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/18chttp://biolab.isis.rl.ac.uk/camerons_labblog/2950/ATIC_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2950/ATIC_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2950/ATIC_10_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2950/ATIC_10_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2950/ATIC_10_mgmL.xml?revision=295029512951ATIC 5 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
ATIC 5 mg/mL




This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T02:44:36+00:002009-02-03T02:44:36+00:0056d797e0ae22aa2fa84203b83df65f310SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/18dhttp://biolab.isis.rl.ac.uk/camerons_labblog/2951/ATIC_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2951/ATIC_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2951/ATIC_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2951/ATIC_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2951/ATIC_5_mgmL.xml?revision=295129522952ATIC 2 mg/mL
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
ATIC 2 mg/mL




This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T02:45:01+00:002009-02-03T02:45:01+00:005ac4e268d7d1979a2b0abaf64357b56b7SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/18ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2952/ATIC_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2952/ATIC_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2952/ATIC_2_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2952/ATIC_2_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2952/ATIC_2_mgmL.xml?revision=295229542954SAX of ATIC buffer
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A32000.203A32001.DAT






This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T03:13:16+00:002009-02-03T03:13:16+00:005b57bd8d5518f8f498fcd70c9f1a2ab66SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/uri/18fhttp://biolab.isis.rl.ac.uk/camerons_labblog/2954/SAX_of_ATIC_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2954/SAX_of_ATIC_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2954/SAX_of_ATIC_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2954/SAX_of_ATIC_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2954/SAX_of_ATIC_buffer.xml?revision=295429552957SAXS on ATIC 20 mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A33000.203A33001.DAT

This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T03:15:16+00:002009-02-03T03:31:05+00:005dba861ea003042e0d8ebd73c15d5157eSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/354.xmlhttp://biolab.isis.rl.ac.uk/uri/190http://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.xml?revision=2955http://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.xml?revision=2956http://biolab.isis.rl.ac.uk/camerons_labblog/2955/SAXS_on_ATIC_20_mgmL.xml?revision=295729582960SAXS on ATIC 2 mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A34000.203A34001.DAT

This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T03:32:23+00:002009-02-03T03:47:34+00:0057262951ef6463aeafd33ab016744fc42SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/356.xmlhttp://biolab.isis.rl.ac.uk/uri/191http://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.xml?revision=2958http://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.xml?revision=2959http://biolab.isis.rl.ac.uk/camerons_labblog/2958/SAXS_on_ATIC_2_mgmL.xml?revision=296029612963SAXS on ATIC 10 mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A35000.203A35001.DAT

This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T04:05:40+00:002009-02-03T04:05:54+00:005047549a6041c7663c0f58c4c9cd1d6afSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/358.xmlhttp://biolab.isis.rl.ac.uk/uri/192http://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.xml?revision=2961http://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.xml?revision=2962http://biolab.isis.rl.ac.uk/camerons_labblog/2961/SAXS_on_ATIC_10_mgmL.xml?revision=296329642966SAXS on ATIC 5 mg/mL
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A36000.203A36001.DAT

This Post is Linked By: SAXS on ATIC; ]]>2009-02-03T04:21:10+00:002009-02-03T04:21:23+00:0056c9f6f04e4ca88b7543b16b41e80f722SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/360.xmlhttp://biolab.isis.rl.ac.uk/uri/193http://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.xml?revision=2964http://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.xml?revision=2965http://biolab.isis.rl.ac.uk/camerons_labblog/2964/SAXS_on_ATIC_5_mgmL.xml?revision=296629672968SAXS on ATIC
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
Project: Soton
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
ATIC - Buffer1090A32000.203SAX of ATIC buffer
ATIC 20 mg/mL1090A33000.203SAXS on ATIC 20 mg/mL
ATIC 2 mg/mL1090A34000.203SAXS on ATIC 2 mg/mL
ATIC 10 mg/mL1090A35000.203SAXS on ATIC 10 mg/mL
ATIC 5 mg/mL1090A36000.203SAXS on ATIC 5 mg/mL

]]>2009-02-03T04:21:49+00:002009-02-03T04:22:06+00:005e860641e14d25dab55de247fddb2ebd5SAXSI22Sotonhttp://biolab.isis.rl.ac.uk/uri/194http://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.xml?revision=2967http://biolab.isis.rl.ac.uk/camerons_labblog/2967/SAXS_on_ATIC.xml?revision=29682971297120 mg/mL ATIC with inhibitor
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
Product 1 of Addition of inhibitor to ATIC solutions




This Post is Linked By: Addition of inhibitor to ATIC solutions;SAXS of ATIC with inhibitors; ]]>2009-02-03T04:58:30+00:002009-02-03T04:58:30+00:0052ea77d4e35116920f6a287039f4837edSolutionSotonhttp://biolab.isis.rl.ac.uk/uri/196http://biolab.isis.rl.ac.uk/camerons_labblog/2971/20_mgmL_ATIC_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2971/20_mgmL_ATIC_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2971/20_mgmL_ATIC_with_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2971/20_mgmL_ATIC_with_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2971/20_mgmL_ATIC_with_inhibitor.xml?revision=29712972297210 mg/mL ATIC with inhibitor
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
Product 2 of Addition of inhibitor to ATIC solutions




This Post is Linked By: Addition of inhibitor to ATIC solutions;SAXS of ATIC with inhibitors; ]]>2009-02-03T04:59:29+00:002009-02-03T04:59:29+00:005132314016cf0a2cb6b491add4a7ebe00SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/197http://biolab.isis.rl.ac.uk/camerons_labblog/2972/10_mgmL_ATIC_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2972/10_mgmL_ATIC_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2972/10_mgmL_ATIC_with_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2972/10_mgmL_ATIC_with_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2972/10_mgmL_ATIC_with_inhibitor.xml?revision=297229702973Addition of inhibitor to ATIC solutions
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Soton
To ATIC 20 mg/mL (100 uL) was added 2 uL of peptide inhibitor to make 20 mg/mL ATIC with inhibitor.

To ATIC 10 mg/mL (100 uL) was added 2 uL of peptide inhibitor to make 10 mg/mL ATIC with inhibitor
This Post is Linked By: 20 mg/mL ATIC with inhibitor;10 mg/mL ATIC with inhibitor; ]]>2009-02-03T04:58:11+00:002009-02-03T04:59:39+00:005b2ae2c5a587ba6d57a57fcd3a265dc5dSotonhttp://biolab.isis.rl.ac.uk/uri/195http://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.xml?revision=2970http://biolab.isis.rl.ac.uk/camerons_labblog/2970/Addition_of_inhibitor_to_ATIC_solutions.xml?revision=297329742977SAXS on 20 mg/mL ATIC plus inhibitor (2%)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A39000.203A39001.DAT

This Post is Linked By: SAXS of ATIC with inhibitors; ]]>2009-02-03T05:21:05+00:002009-02-03T05:43:52+00:005afd598c093d092620b4f8f3476c4950cSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/362.xmlhttp://biolab.isis.rl.ac.uk/uri/198http://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.xml?revision=2974http://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.xml?revision=2976http://biolab.isis.rl.ac.uk/camerons_labblog/2974/SAXS_on_20_mgmL_ATIC_plus_inhibitor_2.xml?revision=297729752979SAXS on 10 mg/mL ATIC plus inhibitor (2%)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A40000.203A40001.DAT

This Post is Linked By: SAXS of ATIC with inhibitors; ]]>2009-02-03T05:22:04+00:002009-02-03T05:51:09+00:0058f4120371f1b868eb0b47e8d71e43ff2SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/364.xmlhttp://biolab.isis.rl.ac.uk/uri/199http://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.xml?revision=2975http://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.xml?revision=2978http://biolab.isis.rl.ac.uk/camerons_labblog/2975/SAXS_on_10_mgmL_ATIC_plus_inhibitor_2.xml?revision=297929822984Second SAXS run on 10 mg/mL with inhibitor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A41000.203A41001.DAT

This Post is Linked By: SAXS of ATIC with inhibitors; ]]>2009-02-03T06:28:16+00:002009-02-03T06:28:30+00:005d3be263e190a4a9cbb2ab66ff07ebd6dSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/366.xmlhttp://biolab.isis.rl.ac.uk/uri/19ahttp://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.xml?revision=2982http://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.xml?revision=2983http://biolab.isis.rl.ac.uk/camerons_labblog/2982/Second_SAXS_run_on_10_mgmL_with_inhibitor.xml?revision=2984298629865 mg/mL ATIC + 10% inhibitor
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
5 mg/mL ATIC + 10% inhibitor prepared in Further preparation of samples with inhibitor




This Post is Linked By: Further preparation of samples with inhibitor;Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T06:59:31+00:002009-02-03T06:59:31+00:00529aa082dd3bcb0551c4ae272412abd48SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/19chttp://biolab.isis.rl.ac.uk/camerons_labblog/2986/5_mgmL_ATIC__10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2986/5_mgmL_ATIC__10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2986/5_mgmL_ATIC__10_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2986/5_mgmL_ATIC__10_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2986/5_mgmL_ATIC__10_inhibitor.xml?revision=29862987298710 mg/mL ATIC with 10% inhibitor
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: Soton
10 mg/mL ATIC with 10% inhibitor prepared in Further preparation of samples with inhibitor




This Post is Linked By: Further preparation of samples with inhibitor;Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T07:00:07+00:002009-02-03T07:00:07+00:0057718c917c9f7646312f9479aaeec2884SolutionSotonhttp://biolab.isis.rl.ac.uk/uri/19dhttp://biolab.isis.rl.ac.uk/camerons_labblog/2987/10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2987/10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2987/10_mgmL_ATIC_with_10_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2987/10_mgmL_ATIC_with_10_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2987/10_mgmL_ATIC_with_10_inhibitor.xml?revision=298729852989Further preparation of samples with inhibitor
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Soton
To ATIC 20 mg/mL (100 uL) was added 10 uL of inhibitor in DMSO to give 5 mg/mL ATIC + 10% inhibitor

To ATIC 10 mg/mL (100 uL) was added 10 uL of inhibitor in DMSO to give 10 mg/mL ATIC with 10% inhibitor
This Post is Linked By: 5 mg/mL ATIC + 10% inhibitor;10 mg/mL ATIC with 10% inhibitor; ]]>2009-02-03T06:59:14+00:002009-02-03T07:08:12+00:00546c4781d4949b805609122b85427ef1aSotonhttp://biolab.isis.rl.ac.uk/uri/19bhttp://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.xml?revision=2985http://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.xml?revision=2988http://biolab.isis.rl.ac.uk/camerons_labblog/2985/Further_preparation_of_samples_with_inhibitor.xml?revision=298929912993SAXS on 5 mg/mL ATIC with 10% inhibitor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A45000.203A45001.DAT

This Post is Linked By: Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T07:23:20+00:002009-02-03T07:55:50+00:005cafe756cff60cf2435a0c7dd64719913SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/368.xmlhttp://biolab.isis.rl.ac.uk/uri/19fhttp://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.xml?revision=2991http://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.xml?revision=2992http://biolab.isis.rl.ac.uk/camerons_labblog/2991/SAXS_on_5_mgmL_ATIC_with_10_inhibitor.xml?revision=299329902994SAXS of 10 mg/mL ATIC with 10% inhibitor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A43000.203A43001.DAT


Somehow the data for this run seemed to get lost due to a computer glitch.
This Post is Linked By: Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T07:22:17+00:002009-02-03T07:56:14+00:0054ebdde5ad6fbe6526000445aabead05dSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/uri/19ehttp://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xml?revision=2990http://biolab.isis.rl.ac.uk/camerons_labblog/2990/SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xml?revision=299430003001SAXS of ATIC with inhibitors
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
Project: Soton
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
20 mg/mL ATIC with inhibitor1090A39000.203SAXS on 20 mg/mL ATIC plus inhibitor (2%)
10 mg/mL ATIC with inhibitor590A40000.203SAXS on 10 mg/mL ATIC plus inhibitor (2%)
10 mg/mL ATIC with inhibitor1090A41000.203Second SAXS run on 10 mg/mL with inhibitor

]]>2009-02-03T08:21:21+00:002009-02-03T08:21:47+00:005c96a44f92083018dc86a9d3e2c705317SAXSI22Sotonhttp://biolab.isis.rl.ac.uk/uri/1a0http://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.xml?revision=3000http://biolab.isis.rl.ac.uk/camerons_labblog/3000/SAXS_of_ATIC_with_inhibitors.xml?revision=300130023004Repeat SAXS of 10 mg/mL ATIC with 10% inhibitor
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A48000.203A48001.DAT

This Post is Linked By: Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T08:39:46+00:002009-02-03T08:53:34+00:0054cea88d1146478ec4ca52304f0ee8bd0SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/370.xmlhttp://biolab.isis.rl.ac.uk/uri/1a1http://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xml?revision=3002http://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xml?revision=3003http://biolab.isis.rl.ac.uk/camerons_labblog/3002/Repeat_SAXS_of_10_mgmL_ATIC_with_10_inhibitor.xml?revision=300430053007Repeat SAXS of 20 mg/mL ATIC
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A49000.203A49001.DAT

This Post is Linked By: Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T08:54:53+00:002009-02-03T09:12:14+00:005b1e681a2f143d542631427459e42a047SAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/data/372.xmlhttp://biolab.isis.rl.ac.uk/uri/1a2http://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.xml?revision=3005http://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.xml?revision=3006http://biolab.isis.rl.ac.uk/camerons_labblog/3005/Repeat_SAXS_of_20_mgmL_ATIC.xml?revision=300730083008Repeat SAXS of ATIC buffer
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Soton

Raw data fileData file
A52000.203A52001.DAT






This Post is Linked By: Continuing SAXS on ATIC with (and without) inhibitor; ]]>2009-02-03T09:34:33+00:002009-02-03T09:34:33+00:00572da3ae0cef0f4823275f43e18dc35ccSAXS_DiamondI22Sotonhttp://biolab.isis.rl.ac.uk/uri/1a3http://biolab.isis.rl.ac.uk/camerons_labblog/3008/Repeat_SAXS_of_ATIC_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3008/Repeat_SAXS_of_ATIC_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3008/Repeat_SAXS_of_ATIC_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3008/Repeat_SAXS_of_ATIC_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3008/Repeat_SAXS_of_ATIC_buffer.xml?revision=300830103010Continuing SAXS on ATIC with (and without) inhibitor
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
Instrument: I22
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
10 mg/mL ATIC with 10% inhibitor1090A44000.203SAXS of 10 mg/mL ATIC with 10% inhibitor
5 mg/mL ATIC + 10% inhibitor1090A44000.203SAXS on 5 mg/mL ATIC with 10% inhibitor
10 mg/mL ATIC with 10% inhibitor1090A48000.203Repeat SAXS of 10 mg/mL ATIC with 10% inhibitor
ATIC 20 mg/mL1090A49000Repeat SAXS of 20 mg/mL ATIC
ATIC - Buffer990A51000.203Repeat SAXS of ATIC buffer






]]>2009-02-03T10:56:21+00:002009-02-03T10:56:21+00:00513eb341d57d1eacb13b4929e25a0342eSAXSI22http://biolab.isis.rl.ac.uk/uri/1a4http://biolab.isis.rl.ac.uk/camerons_labblog/3010/Continuing_SAXS_on_ATIC_with_and_without_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3010/Continuing_SAXS_on_ATIC_with_and_without_inhibitor.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3010/Continuing_SAXS_on_ATIC_with_and_without_inhibitor.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/3010/Continuing_SAXS_on_ATIC_with_and_without_inhibitor.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/3010/Continuing_SAXS_on_ATIC_with_and_without_inhibitor.xml?revision=301075017501test
Note
cameron.neylon.myopenid.comCameron Neylon ]]>2009-03-10T14:20:00+00:002009-03-10T14:20:00+00:005733717840ff1945b4ee514b3b700c4bfhttp://biolab.isis.rl.ac.uk/uri/21fhttp://biolab.isis.rl.ac.uk/camerons_labblog/7501/test.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/7501/test.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/7501/test.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/7501/test.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/7501/test.xml?revision=7501277910237Preparation of lysozyme solutions
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: SAS_standard
Lysozyme (26.3 mg) was dissolved in 1 mL of 20 mM Tris-HCl pH 8.0 (lab stock). The solution was spun at 15 krpm for 10 minutes and then transferred to a fresh tube to give Lysozyme (~20 mg/mL)

From Lysozyme (~20 mg/mL) 200 uL was diluted with 200 uL of buffer to give Lysozyme (~10 mg/mL).

From Lysozyme (~20 mg/mL) 100 uL was diluted with 300 uL of buffer to give Lysozyme (~5 mg/mL)

From Lysozyme (~20 mg/mL) 50 uL was diluted with 450 uL of buffer to give Lysozyme (~2 mg/mL).

Solutions were kept in the cold room overnight.
This Post is Linked By: Lysozyme (~20 mg/mL);Lysozyme (~10 mg/mL);Lysozyme (~5 mg/mL);Lysozyme (~2 mg/mL); ]]>2009-02-01T16:30:55+00:002009-05-29T13:08:32+01:005629a2db598eed099317429e1ad258802SAS_standardhttp://biolab.isis.rl.ac.uk/uri/152http://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.xml?revision=2779http://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.xml?revision=2793http://biolab.isis.rl.ac.uk/camerons_labblog/2779/Preparation_of_lysozyme_solutions.xml?revision=10237278310236Preparation of GFP solutions for I22 SAXS
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: SAS_standard
Previously purified and freeze dried GFP (17.8 mg) was dissolved in 1 mL of 20 mM Tris-HCl pH 8.0 (lab stock). The solution was spun at 14 krpm for ten minutes and then transferred to a fresh tube to give GFP (~20 mg/mL). Significant ppt is obvious after centrifugation so probably lost several miligrams.

From GFP (~10mg/mL) 200 uL was diluted with 200 uL of buffer to give GFP (~10mg/mL).

From GFP (~20 mg/mL) 100 uL was diluted with 300 uL of buffer to give GFP (~5 mg/mL).

From GFP (~20 mg/mL) 50 uL was diluted with 450 uL of buffer to give GFP (~2 mg/mL).
This Post is Linked By: GFP (~20 mg/mL);GFP (~10mg/mL);GFP (~5 mg/mL);GFP (~2 mg/mL); ]]>2009-02-01T16:32:55+00:002009-05-29T13:08:10+01:0053dd9e1135171d61b864977d98eee3455SAS_standardhttp://biolab.isis.rl.ac.uk/uri/154http://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.xml?revision=2783http://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.xml?revision=2784http://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.xml?revision=2789http://biolab.isis.rl.ac.uk/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.xml?revision=10236113311133450 mM Hepes pH 7 Buffer in D2O
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
50 mM Hepes-NaOH pH 7, 100 mM NaCl, 10% glycerol, in D2O


MaterialConcnAmount requiredAmount meas
HEPES 50 mM5.9575g5.9589 g
Sodium Chloride100 mM2.922 g2.9243
Glycerol10%5 g5.0 g


The buffer was made up and pH'd to a reading of 6.608 with NaOD in D2O. pH reading was rising but appeared to settle. May have been slow exchange of glycerol protons.
This Post is Linked By: Dialysis of MurM into new buffer; ]]>2009-06-18T14:09:40+01:002009-06-18T15:03:41+01:00500d33b5c4516cef37ce3e5ce2037575cSolutionhttp://biolab.isis.rl.ac.uk/uri/402http://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.xml?revision=11332http://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.xml?revision=11331http://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.xml?revision=11333http://biolab.isis.rl.ac.uk/camerons_labblog/11331/50_mM_Hepes_pH_7_Buffer_in_D2O.xml?revision=113341133511335MurM sample
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Approx 12 mg/mL MurM in buffer with 50% glycerol 0.5 M NaCl made by Jennifer Shepherd
This Post is Linked By: Dialysis of MurM into new buffer; ]]>2009-06-18T15:04:22+01:002009-06-18T15:04:22+01:00507971f820d0bdb01933ac31f740e0f0aSolutionhttp://biolab.isis.rl.ac.uk/uri/403http://biolab.isis.rl.ac.uk/camerons_labblog/11335/MurM_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11335/MurM_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11335/MurM_sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11335/MurM_sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11335/MurM_sample.xml?revision=113351133711337Dialysed MurM sample in D2O buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: MurM
The sample of MurM after dialysis as described in Dialysis of MurM into new buffer
This Post is Linked By: Dialysis of MurM into new buffer; ]]>2009-06-18T15:18:44+01:002009-06-18T15:18:44+01:0057b4cf9099ed9225b144a21d804d6f195SolutionMurMhttp://biolab.isis.rl.ac.uk/uri/405http://biolab.isis.rl.ac.uk/camerons_labblog/11337/Dialysed_MurM_sample_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11337/Dialysed_MurM_sample_in_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11337/Dialysed_MurM_sample_in_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11337/Dialysed_MurM_sample_in_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11337/Dialysed_MurM_sample_in_D2O_buffer.xml?revision=113371133611344Dialysis of MurM into new buffer
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Sample_preparation
Project: MurM
MurM sample (1 mL) was spun at 6 C at the maximum speed in a bench centrifuge three times ten minutes. No visible aggregate was seen. The sample was then dialysed overnight against ~120 mL of 50 mM Hepes pH 7 Buffer in D2O.

Buffer was changed the following morning and afternoon (Friday). Significant precipitate was observed. On Saturday the remaining buffer was filtered and the sample spun and transferred to new dialysis tubing in filtered buffer and dialysed overnight. A sample was taken for a UV-Vis reading (attached).
Spun sample before final dialysis UV



The final dialysed sample is Dialysed MurM sample in D2O buffer. A sample of the dialysate was taken as a blank for SAXS/SANS:Buffer blank dialysate for MurM
This Post is Linked By: Dialysed MurM sample in D2O buffer;Buffer blank dialysate for MurM; ]]>2009-06-18T15:15:51+01:002009-06-20T21:24:18+01:0053baf59a71535ebdf7b106072d1986e81Sample_preparationMurMhttp://biolab.isis.rl.ac.uk/data/1171.xmlhttp://biolab.isis.rl.ac.uk/uri/404http://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xml?revision=11336http://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xml?revision=11339http://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xml?revision=11343http://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xml?revision=11342http://biolab.isis.rl.ac.uk/camerons_labblog/11336/Dialysis_of_MurM_into_new_buffer.xml?revision=113441153011530Test solution sample
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: test
Test




]]>2009-07-29T03:00:47+01:002009-07-29T03:00:47+01:005b62e5a02219c114e04bc22d1fa5965b9Solutiontesthttp://biolab.isis.rl.ac.uk/uri/457http://biolab.isis.rl.ac.uk/camerons_labblog/11530/Test_solution_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11530/Test_solution_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11530/Test_solution_sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11530/Test_solution_sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11530/Test_solution_sample.xml?revision=115301151011510Approaching the design of a dataset consisting of modelled data based on pdb structures
Note
cameron.neylon.myopenid.comCameron NeylonProject: Data_analysis
Aim: For the set of unique sequences of data obtained from crystallography from the PDB, generate predicted data from a set of techniques. Hold that data in a useful and coordinated form to enable the comparison of (ultimately) experimental data from the same techniques to those systems.

Methods:

The techniques (and tools for generating predicted data) are Small Angle X-ray Scattering (Crysol - ATSAS suite), Small Angle Neutron Scattering (Cryson) and AUC (Hydropro). These programs take PDB files and generate a set of flat text files and (at least for Crysol and Cryson) are controlled from the command line. The file sizes aren't huge and could probably be held as text in a straightforward relational database but a document oriented DB might be better. Conversely could just use a single directory for each PDB and just hold reference data in a DB.

There is a list of sets of sequence-unique PDB IDs at http://swift.cmbi.kun.nl/whatif/select/ These can relatively easily be pulled down by name from the PDB (ftp://ftp.wwpdb.org/pub/pdb/README) as either PDB or XML. As the programs take PDB files but the XML might be more useful further down the track it may be worthwhile to get both. This shouldn't require too much hacking to do via Python.

Doing the actual analysis is a question of scripting the command line operations to run the program so not too hard hopefully. The files will be generated so they may as well sit in the same directory as the PDB file as this will be the default anyway. May need to move them later. Step two would be to pull that information into some sort of useful datastructure but maybe that is better left until the actual files have been generated anyway.

The analysis will involve taking a test set of data and finding the closest match in the full dataset. This may involve truncating and scaling the data to fit the modelled data set. The mechanics of how best to do the comparison are part of the point of the exercise as will be adding noise to see where problems arise.

As always the question is also how best to record and track everything that is going on. A massive GitHub repository is tempting but probably overkill and may run into issues of cost not to mention transfer times. Would be great to write out triples directly to e.g. Talis but in first instance at least writing out a logfile of exactly what happened would be a good start. Need to think about how best to mark that up.
]]>2009-07-24T14:05:39+01:002009-07-24T14:05:39+01:005ae16414ccbc8d4452626e3b12997ff4cData_analysishttp://biolab.isis.rl.ac.uk/uri/44chttp://biolab.isis.rl.ac.uk/camerons_labblog/11510/Approaching_the_design_of_a_dataset_consisting_of_modelled_data_based_on_pdb_structures.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11510/Approaching_the_design_of_a_dataset_consisting_of_modelled_data_based_on_pdb_structures.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11510/Approaching_the_design_of_a_dataset_consisting_of_modelled_data_based_on_pdb_structures.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11510/Approaching_the_design_of_a_dataset_consisting_of_modelled_data_based_on_pdb_structures.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11510/Approaching_the_design_of_a_dataset_consisting_of_modelled_data_based_on_pdb_structures.xml?revision=115101133811529Buffer blank dialysate for MurM
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
A sample of dialysate obtained for use as a buffer blank after Dialysis of MurM into new buffer
This Post is Linked By: Dialysis of MurM into new buffer; ]]>2009-06-18T15:19:26+01:002009-07-29T03:00:08+01:00583cd229b3d38443a23218e283ad3265dSolutionhttp://biolab.isis.rl.ac.uk/uri/406http://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.xml?revision=11338http://biolab.isis.rl.ac.uk/camerons_labblog/11338/Buffer_blank_dialysate_for_MurM.xml?revision=115291157311575Reprocessed Glur0 buffer background
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A19000.202Left/A19001.DAT

This Post is Linked By: Reprocessing SAXS data from Feb 09;Initial analysis of reprocessed Glur0 Data; ]]>2009-08-26T12:48:52+01:002009-08-26T12:50:00+01:005963e5231247503e904964860b9bf565cSAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/1326.xmlhttp://biolab.isis.rl.ac.uk/uri/475http://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.xml?revision=11573http://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.xml?revision=11574http://biolab.isis.rl.ac.uk/camerons_labblog/11573/Reprocessed_Glur0_buffer_background.xml?revision=115751157611578Reprocessed Glur0 buffer background (Right)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: GluR0

Raw data fileData file
A19000.202Right/A19001.DAT

This Post is Linked By: Reprocessing SAXS data from Feb 09;Initial analysis of reprocessed Glur0 Data; ]]>2009-08-26T12:50:44+01:002009-08-26T12:51:16+01:0054f542ca550238e984c8fd1a03793c4c2SAXS_DiamondI22GluR0http://biolab.isis.rl.ac.uk/data/1328.xmlhttp://biolab.isis.rl.ac.uk/uri/476http://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.xml?revision=11576http://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.xml?revision=11577http://biolab.isis.rl.ac.uk/camerons_labblog/11576/Reprocessed_Glur0_buffer_background_Right.xml?revision=115781158011582Reprocessed Glur0 data 1mg/mL (Left)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Glur0

Raw data fileData file
A20000.202A20001.DAT

This Post is Linked By: Reprocessing SAXS data from Feb 09;Initial analysis of reprocessed Glur0 Data; ]]>2009-08-26T12:52:22+01:002009-08-26T12:53:07+01:0055f33888a4e32bf3c38db6df24b66a35dSAXS_DiamondI22Glur0http://biolab.isis.rl.ac.uk/data/1330.xmlhttp://biolab.isis.rl.ac.uk/uri/477http://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.xml?revision=11580http://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.xml?revision=11581http://biolab.isis.rl.ac.uk/camerons_labblog/11580/Reprocessed_Glur0_data_1mgmL_Left.xml?revision=115821158311585Reprocessed Glur0 data 1mg/mL (Right)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: Glur0

Raw data fileData file
A20000.202Right/A20001.DAT

This Post is Linked By: Reprocessing SAXS data from Feb 09;Initial analysis of reprocessed Glur0 Data; ]]>2009-08-26T12:53:50+01:002009-08-26T12:54:19+01:0055518b0aae0eec206847fb525f0a21b27SAXS_DiamondI22Glur0http://biolab.isis.rl.ac.uk/data/1332.xmlhttp://biolab.isis.rl.ac.uk/uri/478http://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.xml?revision=11583http://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.xml?revision=11584http://biolab.isis.rl.ac.uk/camerons_labblog/11583/Reprocessed_Glur0_data_1mgmL_Right.xml?revision=115851158611586Reprocessing SAXS data from Feb 09
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: SAXS
The SAXS data taken in capillaries from the Feb 09 experiment was reprocessed in two halves to reduce the effect of flare from the capillary curvature that was seen in the image. Two 1d reduced datasets were created from each pattern on the left and right of the flare from the image.


SampleRaw dataLeft reduced dataRight reduced data
Glur0 bufferSAXS of GLuR0 bufferReprocessed Glur0 buffer backgroundReprocessed Glur0 buffer background (Right)
Glur0 1mg/mLSAXS of 1 mg/mL GluR0 no glutamateReprocessed Glur0 data 1mg/mL (Left)Reprocessed Glur0 data 1mg/mL (Right)

]]>2009-08-26T12:56:23+01:002009-08-26T12:56:23+01:005288d81711885aa692b102e7012c8983aSAXShttp://biolab.isis.rl.ac.uk/uri/479http://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.xml?revision=115861158711590Glur0 1mg/mL I vs Q (background subtracted and small linear displacement)
Data
cameron.neylon.myopenid.comCameron NeylonData Type: SAXS_Diamond
Project: Glur0
Data generated as described in Initial analysis of reprocessed Glur0 Data

Glur0 1mg/mL I vs Q

This Post is Linked By: Initial analysis of reprocessed Glur0 Data; ]]>2009-08-26T13:03:07+01:002009-08-26T13:04:50+01:005332acddb0fe9e0563c48e78d99f36fb1SAXS_DiamondGlur0http://biolab.isis.rl.ac.uk/data/1334.xmlhttp://biolab.isis.rl.ac.uk/uri/47ahttp://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.xml?revision=11588http://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.xml?revision=11587http://biolab.isis.rl.ac.uk/camerons_labblog/11587/Glur0_1mgmL_I_vs_Q_background_subtracted_and_small_linear_displacement.xml?revision=115901158911598Initial analysis of reprocessed Glur0 Data
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Data_Analysis
Project: Glur0
The data from Reprocessed Glur0 buffer background, Reprocessed Glur0 buffer background (Right), Reprocessed Glur0 data 1mg/mL (Left) andReprocessed Glur0 data 1mg/mL (Right) was loaded into Igor Pro. Only the first six shots (columns) of each dataset were taken based on the apparent degradation of the protein after six shots.

Left and right datasets from the first six shots were added together for both sample and background and the background subtracted from the sample. Because the background was slightly higher in counts at higher Q than the sample 20 counts was added at all Q points to the sample data for visualisation and analysis. The data were plotted and seem to give a good curve for a Q range from 0.005 to around 0.2 inverse angstroms.

Glur0 1mg/mL I vs Q


A quick Guinier analysis gave slopes varying from around 605 to 660 corresponding to an Rg between 43 and 50 angstroms.

Glur0 1mg/mL Guinier


The Igor log file is attached along with images of the graphs. The output data is in Glur0 1mg/mL I vs Q (background subtracted and small linear displacement). This is broadly consistent with the DLS data recorded by Luke in DLS data of GluR0 samples from LoQ Beamtime which gave an Rh of ~72 A which compares well with a spherical R (sqrt(5/3)*Rg) of ~58 from the SAXS data
This Post is Linked By: Glur0 1mg/mL I vs Q (background subtracted and small linear displacement); ]]>2009-08-26T13:04:27+01:002009-08-26T13:36:54+01:0055dfb92f2901828081a779889278a11f1Data_AnalysisGlur0http://biolab.isis.rl.ac.uk/data/1336.xmlhttp://biolab.isis.rl.ac.uk/data/1338.xmlhttp://biolab.isis.rl.ac.uk/data/1340.xmlhttp://biolab.isis.rl.ac.uk/data/1342.xmlhttp://biolab.isis.rl.ac.uk/uri/47bhttp://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11589http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11592http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11591http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11593http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11594http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11595http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11596http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=11597http://biolab.isis.rl.ac.uk/camerons_labblog/11589/Initial_analysis_of_reprocessed_Glur0_Data.xml?revision=115981162111625Calculation of Scattering Length Densities for King/Frey Surfactants
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: Frey-King_Surfactants
In RB910360 A sea surface surfactant model was examined at air water interfaces. The model molecule was N-Me-Phenylalanine C18 ester and this was prepared in both fully hydrogenated and tail deuterated forms.

The following predicted properties were obtained from http://molinspiration.com (volume), and from http://www.ncnr.nist.gov/resources/sldcalc.html (SLD)


ComponentVolume (cubic Angstroms)MW(H)MW(D)SLD-HSLD-D
Full molecule4794464824.19E-75.14E-6
C18-O (tail)317267303-1.73E-76.91E-6
N-Phe-OMe173179N/A1.49E-6N/A


In addition to the pure molecules two samples were made in which tail-D and fully-H samples of surfactant were mixed. These were made in a nominal ration of 1:1 and 1:3 by weight. The notional SLDs for each of these samples are.


SampleTail SLDHead SLD
Fully-H-1.7E-71.34E-6
Tail-D6.91E-61.34E-6
1:1 D:H3.23E-61.34E-6
1:3 D:H1.49E-61.34E-6

]]>2009-09-10T12:17:36+01:002009-09-10T14:35:18+01:00578a70cafadb1ada8674f45690b523cbeFrey-King_Surfactantshttp://biolab.isis.rl.ac.uk/uri/48ehttp://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xml?revision=11621http://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xml?revision=11622http://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xml?revision=11623http://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xml?revision=11624http://biolab.isis.rl.ac.uk/camerons_labblog/11621/Calculation_of_Scattering_Length_Densities_for_KingFrey_Surfactants.xml?revision=116251167011670Purified GFP lyophilised
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Powder
Purified GFP (Ni-column and gel filtered) prepared by Luke.
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T09:25:56+01:002009-09-25T09:25:56+01:005a53c2f5c0b48408d4e64af1246e32ec1Powderhttp://biolab.isis.rl.ac.uk/uri/4a1http://biolab.isis.rl.ac.uk/camerons_labblog/11670/Purified_GFP_lyophilised.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11670/Purified_GFP_lyophilised.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11670/Purified_GFP_lyophilised.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11670/Purified_GFP_lyophilised.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11670/Purified_GFP_lyophilised.xml?revision=116701167511675H2O Phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
50 mM Phoshate buffer pH 7 in H2O


]]>2009-09-25T10:01:50+01:002009-09-25T10:01:50+01:005e3cdb31db1719f8d4597fe86f619b501SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a3http://biolab.isis.rl.ac.uk/camerons_labblog/11675/H2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11675/H2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11675/H2O_Phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11675/H2O_Phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11675/H2O_Phosphate_buffer.xml?revision=116751167611676D2O Phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
50 mM Phosphate buffer pD 7 in D2O


]]>2009-09-25T10:02:19+01:002009-09-25T10:02:19+01:005f71850171e27aab3d7d1ed3cdff8d0b8SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a4http://biolab.isis.rl.ac.uk/camerons_labblog/11676/D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11676/D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11676/D2O_Phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11676/D2O_Phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11676/D2O_Phosphate_buffer.xml?revision=116761167711684Preparing samples of GFP and Lysozyme for SANS2d experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonProject: SANS2dfirstbio
First biology on SANS2d! We will run samples of GFP and lysozyme at ~5mg/mL in D2O, H2O, and 70% D2O (contrast match of DNA). Will take around 10 mg of each and dissolve in 2 mL of H2O and D2O and then spin hard to remove aggregate. Then samples will be made up to order. Using 50 mM Na Phosphate buffer pH 7 previously made up by Luke.

~10 mL of buffer was filtered through 0.45um filters. At least 2 mL was pushed though and discarded before the good stuff was kept. This reduces any risk of glycerol or water contamination.


ProteinSolventWeightBuffer volVisible ppt?
LysozymeH2O12.70.77mLN
LysozymeD2O16.51.00 mLN
Purified GFP lyophilisedH2O11.71.17mLY
Purified GFP lyophilisedD2O10.01.00mLY


The samples were dissolved in the given voume of buffer and then spun for 10 minutes 10 20,000g in the cold room in a bench top centrifuge.

Supernatant of all samples was transferred to fresh eppendorfs and the GFP was re-spun. 250 uL of H and D samples were transferred to clean 1mm Helma cells for SANS as follows:


Lysozyme-H2OLysozyme sample in H2O phosphate buffer
Lysozyme-D2OLysozyme sample in D2O phosphate buffer
GFP-H2OGFP in H2O Phosphate buffer
GFP-D2OGFP in D2O Phosphate buffer


From the H and D stocks 175 uL of D-sample was mixed with 75ul of H-sample to give 250uL of 70% D2O sample in 1 mm helma cuvettes.


Lysozyme-70% D2OLysozyme sample in 70% D2O phosphate buffer
GFP-70% D2OGFP in 70% D2O Phosphate buffer

This Post is Linked By: GFP in D2O Phosphate buffer;Lysozyme sample in H2O phosphate buffer;Lysozyme sample in D2O phosphate buffer;GFP in H2O Phosphate buffer;GFP in 70% D2O Phosphate buffer;Lysozyme sample in 70% D2O phosphate buffer; ]]>2009-09-25T10:32:53+01:002009-09-25T11:39:28+01:005949aa945e42e61c6df71c6f635d2a000SANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a5http://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.xml?revision=11677http://biolab.isis.rl.ac.uk/camerons_labblog/11677/Preparing_samples_of_GFP_and_Lysozyme_for_SANS2d_experiment.xml?revision=116841167911686GFP in D2O Phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
GFP sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:35:08+01:002009-09-25T11:39:56+01:005f84681c823ed3a4b3e728b60cf85d6b0SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a7http://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.xml?revision=11679http://biolab.isis.rl.ac.uk/camerons_labblog/11679/GFP_in_D2O_Phosphate_buffer.xml?revision=116861168011687Lysozyme sample in H2O phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
Lysozyme sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:35:24+01:002009-09-25T11:40:11+01:005a8c5fb0c591d3bdc0f1099a6de21c008SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a8http://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.xml?revision=11680http://biolab.isis.rl.ac.uk/camerons_labblog/11680/Lysozyme_sample_in_H2O_phosphate_buffer.xml?revision=116871168111689Lysozyme sample in D2O phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
Lysozyme sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:35:38+01:002009-09-25T11:40:22+01:005f46754ab1b82662db5297e47edbbb773SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a9http://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.xml?revision=11681http://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.xml?revision=11688http://biolab.isis.rl.ac.uk/camerons_labblog/11681/Lysozyme_sample_in_D2O_phosphate_buffer.xml?revision=116891167811685GFP in H2O Phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
GFP sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:34:52+01:002009-09-25T11:39:47+01:0056438239f4fbe0dfcf53fae140329a6b1SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4a6http://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.xml?revision=11678http://biolab.isis.rl.ac.uk/camerons_labblog/11678/GFP_in_H2O_Phosphate_buffer.xml?revision=116851168211691GFP in 70% D2O Phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
GFP sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:35:54+01:002009-09-25T11:40:32+01:005f819c138ac4e5c65009983df161ab284SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4aahttp://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.xml?revision=11682http://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.xml?revision=11690http://biolab.isis.rl.ac.uk/camerons_labblog/11682/GFP_in_70_D2O_Phosphate_buffer.xml?revision=116911168311692Lysozyme sample in 70% D2O phosphate buffer
Materials
cameron.neylon.myopenid.comCameron NeylonMaterial: Solution
Project: SANS2dfirstbio
Lysozyme sample from Preparing samples of GFP and Lysozyme for SANS2d experiment
This Post is Linked By: Preparing samples of GFP and Lysozyme for SANS2d experiment; ]]>2009-09-25T11:36:08+01:002009-09-25T11:40:45+01:005b82ab3bf38fe6a7f80b61bc98fe62c89SolutionSANS2dfirstbiohttp://biolab.isis.rl.ac.uk/uri/4abhttp://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.xml?revision=11683http://biolab.isis.rl.ac.uk/camerons_labblog/11683/Lysozyme_sample_in_70_D2O_phosphate_buffer.xml?revision=116921185411856Luke's PinA data
Data
cameronneylon.netCameron NeylonData Type: SANS
SANS data collected at the LLB by Luke and others some time ago.

7.1 mg.mL PinA Data

This Post is Linked By: Quick analysis of Lukes PinA data; ]]>2009-11-23T16:01:19+00:002009-11-23T16:02:16+00:005c7e08d91d475b91f883cee24452d262aSANShttp://biolab.isis.rl.ac.uk/data/1529.xmlhttp://biolab.isis.rl.ac.uk/uri/4f7http://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.xml?revision=11854http://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.xml?revision=11855http://biolab.isis.rl.ac.uk/camerons_labblog/11854/Lukes_PinA_data.xml?revision=118561185711863Quick analysis of Lukes PinA data
Procedures
cameronneylon.netCameron Neylon-1 and the protein SLD set at 3.14E-6 cm-1. Allowing the protein SLD to float along with radius in the fit, with fixed solvent and scale parameter gave very consistent results confirming this fit to be physically sensible. Chi squared was around 20. [data]1537[/data] Coefficient values ± one standard deviation Scale fact. =3.2652e-08 ± 0 Radius =39.253 ± 0.768 Length =364.99 ± 59.1 Prot SLD =3.14e-06 ± 4.52e-08 Solv SLD =6.3e-06 ± 0 Bgd =2.1875e-06 ± 4.54e-08 V_chisq = 19.9057 coef_cyl[0]= {3.26521e-08,39.253,364.991,3.14003e-06,6.3e-06,2.18753e-06} W_sigma[0]= {0,0.768138,59.0652,4.52347e-08,0,4.53678e-08} The hollow cylinder model gave reasonable fits with similar chi squared but with the protein and solvent SLD fixed the fits tended to give an internal cylinder diameter that tried to tend to zero, good fits gave values of less than 0.1 A which doesn't make physical sense. The large error on R1 is also consistent with an internal radius being poorly determined by the data, suggesting in turn of that fact that there isn't one! Coefficient values ± one standard deviation Scale fact =3.1412e-08 ± 4.39e-09 Radius 1 =0.090734 ± 537 Radius 2 =39.858 ± 1.9 Length =352.84 ± 54.8 Prot SLD =3.14e-06 ± 0 Solv SLD =6.3e-06 ± 0 Bgd =2.2003e-06 ± 4.43e-08 V_chisq = 22.2954 coef_Hcyl[0]= {3.14117e-08,0.0907338,39.8583,352.842,3.14e-06,6.3e-06,2.20034e-06} W_sigma[0]= {4.39491e-09,536.55,1.90302,54.7849,0,0,4.42839e-08} The core-shell model gave marginally better fits but required a SLD for the core of less than -8E-6 which does not make physical sense. A sensible value would be somewhere between the protein and solvent SLD for a highly hydrated protein segment. Coefficient values ± one standard deviation Scale fact =7.3441e-09 ± 8.13e+09 Radius 1 =13.539 ± 7.81 Radius 2 =37.932 ± 2.25 Length =272.04 ± 0.0495 SLD core =-3.1389e-05 ± 1.95e+06 SLD shell =3.14e-06 ± 2.88e+07 SLD solv =6.3e-06 ± nan Bgd =1.8546e-06 ± nan V_chisq = 12.2288 coef_cscyl[0]= {7.34406e-09,13.5393,37.9321,272.043,-3.13891e-05,3.14e-06,6.3e-06,1.85461e-06} W_sigma[0]= {8.12631e+09,7.81095,2.2476,0.0494713,1.94775e+06,2.88406e+07,NaN,NaN} Overall the data suggests strongly that the PinA complex in solution is a homogenous cylinder with a radius of about 40 A and a length of 350-400 A.]]>Procedure: Data_Analysis
Project: Pins
The data from Luke's PinA data was loaded into Igor Pro and analysed using the SANS fitting routines from NIST. The data was fit to a cylinder model, a hollow cylinder, and a core-shell cylinder.

The cylinder model gave a radius of 39 A, and a length of 364 A, with scattering length density of the solvent set at 6.3E-6 cm-1 and the protein SLD set at 3.14E-6 cm-1. Allowing the protein SLD to float along with radius in the fit, with fixed solvent and scale parameter gave very consistent results confirming this fit to be physically sensible. Chi squared was around 20.

Cylinder Fit graph


Coefficient values ± one standard deviation
Scale fact. =3.2652e-08 ± 0
Radius =39.253 ± 0.768
Length =364.99 ± 59.1
Prot SLD =3.14e-06 ± 4.52e-08
Solv SLD =6.3e-06 ± 0
Bgd =2.1875e-06 ± 4.54e-08
V_chisq = 19.9057
coef_cyl[0]= {3.26521e-08,39.253,364.991,3.14003e-06,6.3e-06,2.18753e-06}
W_sigma[0]= {0,0.768138,59.0652,4.52347e-08,0,4.53678e-08}

The hollow cylinder model gave reasonable fits with similar chi squared but with the protein and solvent SLD fixed the fits tended to give an internal cylinder diameter that tried to tend to zero, good fits gave values of less than 0.1 A which doesn't make physical sense. The large error on R1 is also consistent with an internal radius being poorly determined by the data, suggesting in turn of that fact that there isn't one!

Coefficient values ± one standard deviation
Scale fact =3.1412e-08 ± 4.39e-09
Radius 1 =0.090734 ± 537
Radius 2 =39.858 ± 1.9
Length =352.84 ± 54.8
Prot SLD =3.14e-06 ± 0
Solv SLD =6.3e-06 ± 0
Bgd =2.2003e-06 ± 4.43e-08
V_chisq = 22.2954
coef_Hcyl[0]= {3.14117e-08,0.0907338,39.8583,352.842,3.14e-06,6.3e-06,2.20034e-06}
W_sigma[0]= {4.39491e-09,536.55,1.90302,54.7849,0,0,4.42839e-08}


The core-shell model gave marginally better fits but required a SLD for the core of less than -8E-6 which does not make physical sense. A sensible value would be somewhere between the protein and solvent SLD for a highly hydrated protein segment.

Coefficient values ± one standard deviation
Scale fact =7.3441e-09 ± 8.13e+09
Radius 1 =13.539 ± 7.81
Radius 2 =37.932 ± 2.25
Length =272.04 ± 0.0495
SLD core =-3.1389e-05 ± 1.95e+06
SLD shell =3.14e-06 ± 2.88e+07
SLD solv =6.3e-06 ± nan
Bgd =1.8546e-06 ± nan
V_chisq = 12.2288
coef_cscyl[0]= {7.34406e-09,13.5393,37.9321,272.043,-3.13891e-05,3.14e-06,6.3e-06,1.85461e-06}
W_sigma[0]= {8.12631e+09,7.81095,2.2476,0.0494713,1.94775e+06,2.88406e+07,NaN,NaN}

Overall the data suggests strongly that the PinA complex in solution is a homogenous cylinder with a radius of about 40 A and a length of 350-400 A.
]]>2009-11-23T16:10:02+00:002009-11-23T16:24:15+00:0055b28d2687ae608a5be6e397355dc6f47Data_AnalysisPinshttp://biolab.isis.rl.ac.uk/data/1531.xmlhttp://biolab.isis.rl.ac.uk/data/1533.xmlhttp://biolab.isis.rl.ac.uk/data/1537.xmlhttp://biolab.isis.rl.ac.uk/uri/4f8http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11858http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11857http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11859http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11860http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11861http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=11862http://biolab.isis.rl.ac.uk/camerons_labblog/11857/Quick_analysis_of_Lukes_PinA_data.xml?revision=118631191011912GFP SANS Data from SANS2d - 6m D2O
Data
cameronneylon.netCameron NeylonData Type: SANS_SANS2d
The SANS data determined in #####
This Post is Linked By: Comparison of SANS2d and LOQ GFP data; ]]>2009-12-09T11:16:46+00:002009-12-09T11:22:16+00:00538ab8b63b61bd6fa4abde35400c965b5SANS_SANS2dhttp://biolab.isis.rl.ac.uk/data/1567.xmlhttp://biolab.isis.rl.ac.uk/uri/50bhttp://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.xml?revision=11910http://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.xml?revision=11911http://biolab.isis.rl.ac.uk/camerons_labblog/11910/GFP_SANS_Data_from_SANS2d__6m_D2O.xml?revision=119121191811920Lysozyme in D2O data from SANS2D
Data
cameronneylon.netCameron NeylonData Type: SANS_SANS2d
Data recorded on SANS2d in ******
This Post is Linked By: Comparison of SANS2d and LOQ Lysozyme data; ]]>2009-12-09T11:35:34+00:002009-12-09T11:36:23+00:005d580677283a57032fcb282e00d4b73b8SANS_SANS2dhttp://biolab.isis.rl.ac.uk/data/1573.xmlhttp://biolab.isis.rl.ac.uk/uri/50dhttp://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.xml?revision=11918http://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.xml?revision=11919http://biolab.isis.rl.ac.uk/camerons_labblog/11918/Lysozyme_in_D2O_data_from_SANS2D.xml?revision=119201192111925Comparison of SANS2d and LOQ Lysozyme data
Procedures
cameronneylon.netCameron NeylonProcedure: Data_Analysis
The data from Lysozyme in D2O data from SANS2D was compared to data obtained on LOQ 44703 - 10mg/mL Lysozyme on LOQ.

LOQ data was scaled by 1.2 and compared to the SANS2D data. This was compared to a predicted pattern but as I have failed to note the precise crystal structure I used to predict this it isn't much use. The comparison of the two datasets looks pretty good. It should be noted that this is data from two different samples and that the SANS2d scaling is still being worked out in detail.


Lysozyme comparison

]]>2009-12-09T11:38:28+00:002009-12-09T16:52:43+00:0053eee989e99e73acb1ef7fdc6c118c8c0Data_Analysishttp://biolab.isis.rl.ac.uk/data/1577.xmlhttp://biolab.isis.rl.ac.uk/data/1581.xmlhttp://biolab.isis.rl.ac.uk/uri/50ehttp://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xml?revision=11922http://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xml?revision=11921http://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xml?revision=11923http://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xml?revision=11924http://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.xml?revision=119251191311926Comparison of SANS2d and LOQ GFP data
Procedures
cameronneylon.netCameron NeylonProcedure: Data_Analysis
Two datasets were compared and graphed. The SANS2d dataset GFP SANS Data from SANS2d - 6m D2O was compared to the old LOQ dataset GFP (10 mg/mL) in D2O buffer and graphed to give the following.

LOQ and SANS2d GFP comparison


The LOQ data was scaled by a factor of 0.72 (see log file for details) to fit onto the SANS2d data. The fit looks pretty good and will need to be compared to that predicted from the correct crystal structure. The low Q rise in the SANS2d data is almost certainly due to aggregation. It should be noted that this is data from two different samples and that the SANS2d scaling is still being worked out in detail.
]]>2009-12-09T11:23:14+00:002009-12-09T16:53:03+00:00576dc352e8e139cd24f7a9451effc5169Data_Analysishttp://biolab.isis.rl.ac.uk/data/1569.xmlhttp://biolab.isis.rl.ac.uk/data/1571.xmlhttp://biolab.isis.rl.ac.uk/uri/50chttp://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=11914http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=11915http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=11913http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=11916http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=11917http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.xml?revision=119261192811938Some analysis of reflection data from Crisp Experiment
Procedures
cameron.neylon.myopenid.comCameron NeylonProcedure: Data_Analysis
I have previously done single layer fits of data from these experiments for the following contrasts:

H-tails D2O
D-tails NRW
D-tails 30% D2O
1:3 tails NRW
1:1 tails NRW

These show a reasonably reproducible thickening of the layer for the three surface pressure regimes.

On moving to a two layer model with the 7 mN data the fits really want to minimize the headgroup thickness. Hydration of the headgroup doesn't seem to effect the fit much. Some issues with SLD for the mixed tails, similar to what was seen previously with the single layer fits.

With the parameters taken from a two layer fit of the 7 mN data, the predicted NR fits kind of ok by eye to data from the D-Tail-D2O contrast with no further fitting (chi^2 around 70 for six data sets). Further fitting leads to a very thin (~1.5A) layer with high hydration suggesting a very minimal headgroup layer at this surface pressure.

screenshot from Rascal


Moved on the ~14 nm data and was getting very similar results until I realized I had the SLD for the heads fixed at the wrong value?!? On fixing this I can get reasonable fits with the correct SLD and a layer thickness of 2 A

14 mN two layer fit


After playing around with some roughness it doesn't really help. Hydration tends to go to 100% with a thinner layer. Still not well determined by the data though.

14mN Two layer after roughnesses


Looking at the 7 mN data with the correct SLD for the headgroups it is still difficult to see any evidence of the headgroups in the data. These fits are actually a lot worse than the single layer fits.

7mN-correct-SLD-roughness

]]>2009-12-10T11:36:14+00:002009-12-10T14:50:32+00:00545161d681d8a723ff2d37e0ca80b9af1Data_Analysishttp://biolab.isis.rl.ac.uk/data/1584.xmlhttp://biolab.isis.rl.ac.uk/data/1586.xmlhttp://biolab.isis.rl.ac.uk/data/1588.xmlhttp://biolab.isis.rl.ac.uk/data/1590.xmlhttp://biolab.isis.rl.ac.uk/uri/510http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11928http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11929http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11930http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11931http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11932http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11933http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11934http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11935http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11936http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=11937http://biolab.isis.rl.ac.uk/camerons_labblog/11928/Some_analysis_of_reflection_data_from_Crisp_Experiment.xml?revision=119381213012130Glur0 D2O Sample
Materials
cameronneylon.netCameron NeylonProject: GluR0
GluR0 sample in D20

created from Bulk Purification of GluR0 for SANS Beamtime
]]>2010-03-09T19:31:48+00:002010-03-09T19:31:48+00:0059763f8b4380cc46a11d0d3484ffe4c98GluR0http://biolab.isis.rl.ac.uk/uri/592http://biolab.isis.rl.ac.uk/camerons_labblog/12130/Glur0_D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12130/Glur0_D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12130/Glur0_D2O_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12130/Glur0_D2O_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12130/Glur0_D2O_Sample.xml?revision=12130121321213210mM DM H2O Buffer
Materials
cameronneylon.netCameron NeylonProject: GluR0
20mM Tris 100mM KCl 10mM Decyl maltoside pH 7.6

Used as buffer for Dialysis
This Post is Linked By: Adding runs from March GLur0 SANS Expt; ]]>2010-03-09T19:51:06+00:002010-03-09T19:51:06+00:00583616df5293b79f7df9d34516ef3cc57GluR0http://biolab.isis.rl.ac.uk/uri/594http://biolab.isis.rl.ac.uk/camerons_labblog/12132/10mM_DM_H2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12132/10mM_DM_H2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12132/10mM_DM_H2O_Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12132/10mM_DM_H2O_Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12132/10mM_DM_H2O_Buffer.xml?revision=121321213312133D2O Buffer for Glur0
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
10 mM DM buffer in D2O


This Post is Linked By: Second set of GLur0 samples;First Glur0 runs - SANS2d; ]]>2010-03-09T19:51:07+00:002010-03-09T19:51:07+00:005d9ab29a2084b9631a613b2059745f090SolutionGlur0http://biolab.isis.rl.ac.uk/uri/595http://biolab.isis.rl.ac.uk/camerons_labblog/12133/D2O_Buffer_for_Glur0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12133/D2O_Buffer_for_Glur0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12133/D2O_Buffer_for_Glur0.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12133/D2O_Buffer_for_Glur0.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12133/D2O_Buffer_for_Glur0.xml?revision=121331213412134H2O Buffer for Glur0
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
10 mM DM buffer in H2O


This Post is Linked By: Second set of GLur0 samples;First Glur0 runs - SANS2d; ]]>2010-03-09T19:51:30+00:002010-03-09T19:51:30+00:0059c48bc2f05683d13f7359143cf815595SolutionGlur0http://biolab.isis.rl.ac.uk/uri/596http://biolab.isis.rl.ac.uk/camerons_labblog/12134/H2O_Buffer_for_Glur0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12134/H2O_Buffer_for_Glur0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12134/H2O_Buffer_for_Glur0.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12134/H2O_Buffer_for_Glur0.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12134/H2O_Buffer_for_Glur0.xml?revision=12134121351213510mM DM D2O Buffer
Materials
cameronneylon.netCameron NeylonProject: GluR0
20mM Tris 100mM KCl 10mM DM pD 7.6

Buffer used for final dialysis of the 100% D2O sample
This Post is Linked By: Adding runs from March GLur0 SANS Expt; ]]>2010-03-09T19:52:14+00:002010-03-09T19:52:14+00:00591502c83e030bd6d54ab8d124de72f19GluR0http://biolab.isis.rl.ac.uk/uri/597http://biolab.isis.rl.ac.uk/camerons_labblog/12135/10mM_DM_D2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12135/10mM_DM_D2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12135/10mM_DM_D2O_Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12135/10mM_DM_D2O_Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12135/10mM_DM_D2O_Buffer.xml?revision=1213512139121423333-First Glur0 D2O reduced data
Data
cameronneylon.netCameron NeylonData Type: SANS_SANS2d
First shot reduced data for one hour of collection for sample and buffer.
]]>2010-03-09T22:50:38+00:002010-03-09T22:51:56+00:00508edc29dd1157578c0b7d2a580e0c46eSANS_SANS2dhttp://biolab.isis.rl.ac.uk/data/1703.xmlhttp://biolab.isis.rl.ac.uk/data/1705.xmlhttp://biolab.isis.rl.ac.uk/uri/599http://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.xml?revision=12139http://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.xml?revision=12140http://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.xml?revision=12141http://biolab.isis.rl.ac.uk/camerons_labblog/12139/3333First_Glur0_D2O_reduced_data.xml?revision=121421212512143Glur0 D2O Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A glur0 sample in D2O. Concentration calculated by UV-Vis of 1.92 mg/ml.
This Post is Linked By: Second set of GLur0 samples;First Glur0 runs - SANS2d;Sortase A Assay with GluRO sample at 6C[Samples are belong to Cameron];Adding runs from March GLur0 SANS Expt; ]]>2010-03-09T19:13:11+00:002010-03-09T23:15:38+00:005fc8705a92b09f217980f2665c1dae96aSolutionGlur0http://biolab.isis.rl.ac.uk/uri/590http://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.xml?revision=12125http://biolab.isis.rl.ac.uk/camerons_labblog/12125/Glur0_D2O_Sample.xml?revision=121431212612144Glur0 H2O Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A sample of Glur0 in H2O. Concentration of 2.77 mg/ml

Sample created from Bulk Purification of GluR0 for SANS Beamtime
This Post is Linked By: Second set of GLur0 samples;Single run of Glur0 H2O;First Glur0 runs - SANS2d;Adding runs from March GLur0 SANS Expt; ]]>2010-03-09T19:13:35+00:002010-03-09T23:17:34+00:0055df5eb1d9c633bbbd671a40e97adf25cSolutionGlur0http://biolab.isis.rl.ac.uk/data/1701.xmlhttp://biolab.isis.rl.ac.uk/uri/591http://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xml?revision=12126http://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xml?revision=12127http://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xml?revision=12128http://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xml?revision=12129http://biolab.isis.rl.ac.uk/camerons_labblog/12126/Glur0_H2O_Sample.xml?revision=121441215012152Second set of GLur0 samples
Procedures
cameronneylon.netCameron NeylonProcedure: SANS
Instrument: SANS2d
Project: Glur0
The following samples were run as described on SANS2d at ISIS. The samples were run in 1 mm path length rectangular cells (200 uL samples) at a temperature of 20 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
GlurO + glutamate D2OT63335
H2O Buffer for Glur0S403336
D2O Buffer for Glur0S403337
GlurO + glutamate D2OS403338
Glur0 D2O SampleS403339
Glur0 H2O SampleS403340
H2O Buffer for Glur0S403341
D2O Buffer for Glur0S403342
GlurO + glutamate D2OS403343


GCL script

]]>2010-03-10T10:09:23+00:002010-03-10T10:10:15+00:005d951672d990cea9ec9a5a9bddc616c2fSANSSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1710.xmlhttp://biolab.isis.rl.ac.uk/uri/59chttp://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.xml?revision=12151http://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.xml?revision=12150http://biolab.isis.rl.ac.uk/camerons_labblog/12150/Second_set_of_GLur0_samples.xml?revision=121521215512155Comments on Glur0 experiment in progress
Note
cameronneylon.netCameron NeylonProject: Glur0
Data so far looks reasonably good for one hour runs in D2O and pretty ropey for one hour runs in H2O. This is not surprising. More worrying is that the scattering from the sample with glutamate looks remarkably similar to that for detergent on its own. Mike is going to do FTIR on the samples to quantify DM in each sample which should allow us to substract/correct properly.

Plan is to proceed with the contrast variation series for the unbound (no glutamate) samples. Data still needs to be properly reduced (added up etc.) as of 10am Wed 10th
]]>2010-03-10T10:15:08+00:002010-03-10T10:15:08+00:005666ec4d4e04d3e07613a32f7d6f63588Glur0http://biolab.isis.rl.ac.uk/uri/59ehttp://biolab.isis.rl.ac.uk/camerons_labblog/12155/Comments_on_Glur0_experiment_in_progress.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12155/Comments_on_Glur0_experiment_in_progress.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12155/Comments_on_Glur0_experiment_in_progress.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12155/Comments_on_Glur0_experiment_in_progress.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12155/Comments_on_Glur0_experiment_in_progress.xml?revision=121551215612156Glur0 in 22% D2O buffer
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
Unliganded Glur0 in 22% D2O buffer

Linked Posts
]]>2010-03-10T11:18:04+00:002010-03-10T11:18:04+00:00541fe2bf4285b030478113228fea4ae52SolutionGlur0http://biolab.isis.rl.ac.uk/uri/59fhttp://biolab.isis.rl.ac.uk/camerons_labblog/12156/Glur0_in_22_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12156/Glur0_in_22_D2O_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12156/Glur0_in_22_D2O_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12156/Glur0_in_22_D2O_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12156/Glur0_in_22_D2O_buffer.xml?revision=12156121571215722% D2O Buffer
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
10 mM DM buffer in 22% D2O

Linked Posts
]]>2010-03-10T11:18:31+00:002010-03-10T11:18:31+00:0054e7db2abae8532aa031df86665f5ce00SolutionGlur0http://biolab.isis.rl.ac.uk/uri/5a0http://biolab.isis.rl.ac.uk/camerons_labblog/12157/22_D2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12157/22_D2O_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12157/22_D2O_Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12157/22_D2O_Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12157/22_D2O_Buffer.xml?revision=121571215312159Single run of Glur0 H2O
Procedures
cameronneylon.netCameron NeylonProcedure: SANS
Instrument: SANS2d
Project: Glur0
The following samples were run as described on SANS2d at ISIS. The samples were run in 1 mm path length rectangular cells (200 uL samples) at a temperature of 20 C. Script is Cameron_6m_6.gcl (to be attached)


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Glur0 H2O SampleS403345


GCL script

]]>2010-03-10T10:12:02+00:002010-03-10T11:20:51+00:005dbd54e6ca1bf14bb5bfc707c89cc9723SANSSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1712.xmlhttp://biolab.isis.rl.ac.uk/uri/59dhttp://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.xml?revision=12153http://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.xml?revision=12154http://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.xml?revision=12158http://biolab.isis.rl.ac.uk/camerons_labblog/12153/Single_run_of_Glur0_H2O.xml?revision=121591216912172Glur0 H2O summed data
Data
cameronneylon.netCameron NeylonData Type: SANS_SANS2d
Instrument: SANS2d
Project: Glur0

Raw data fileData file
3334, 3340, 3345 - 3336, 3341


loq format
cansas xml format

]]>2010-03-10T18:32:58+00:002010-03-10T18:33:35+00:005d00cef230bdac64b8d549c667a2d7294SANS_SANS2dSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1720.xmlhttp://biolab.isis.rl.ac.uk/data/1722.xmlhttp://biolab.isis.rl.ac.uk/uri/5a3http://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.xml?revision=12169http://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.xml?revision=12170http://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.xml?revision=12171http://biolab.isis.rl.ac.uk/camerons_labblog/12169/Glur0_H2O_summed_data.xml?revision=121721216512173Glur0 D2O summed data
Data
cameronneylon.netCameron NeylonData Type: SANS_SANS2d
Instrument: SANS2d
Project: Glur0

Raw data fileData file
3333, 3339 - 3325, 3337, 3342


q data
cansas xml

]]>2010-03-10T18:30:21+00:002010-03-10T18:33:58+00:00585b46cab1d270c57812645ea4c1d92f0SANS_SANS2dSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1716.xmlhttp://biolab.isis.rl.ac.uk/data/1718.xmlhttp://biolab.isis.rl.ac.uk/uri/5a2http://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xml?revision=12165http://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xml?revision=12166http://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xml?revision=12167http://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xml?revision=12168http://biolab.isis.rl.ac.uk/camerons_labblog/12165/Glur0_D2O_summed_data.xml?revision=121731214512176GlurO + glutamate D2O
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: GlurO
A sample of GlurO in 200 uM glutamate, 10 mM DM. With a concentration of 1.8 mg / ml. SANS data indicated the decyl maltoside concentration was high so FTIR was used the compare the CH2 assymetrical regions between the buffer at 10 mM DM and the sample with an unknown DM concentration. Relative peak areas indicated that the DM concentration in the sample was 116 mM DM (see attached data image)
This Post is Linked By: Second set of GLur0 samples;Sortase A Assay with GluRO sample at 6C[Samples are belong to Cameron]; ]]>2010-03-09T23:21:43+00:002010-03-10T18:36:44+00:005f3f9efd81e4b312b5d425c282ec826fdSolutionGlurOhttp://biolab.isis.rl.ac.uk/data/1724.xmlhttp://biolab.isis.rl.ac.uk/uri/59ahttp://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.xml?revision=12145http://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.xml?revision=12174http://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.xml?revision=12175http://biolab.isis.rl.ac.uk/camerons_labblog/12145/GlurO__glutamate_D2O.xml?revision=121761226612266Transformation of pSOT092 and pLLC146 into BL21(DE3)
Procedures
cameronneylon.netCameron NeylonProcedure: Transformation
Plasmid samples of pLLC146 (EGFP-LPETGG-His6) and pSOT092 (His6-sortase) were transformed into BL21(DE3) competent cells (Novagen).

1 uL of plasmid was added to the competent cells and incubated for 30 minutes on ice. The cells were then heatshocked at 42 deg for 30 seconds and placed back on ice before adding warm SOC medium and incubating at 37 C for one hour. The culture (50 uL) was then plated onto LB plates with 100 ug/mL ampicillin.
]]>2010-04-09T12:22:22+01:002010-04-09T12:22:22+01:00578ec747e258ecc42195ed1b9df377c80Transformationhttp://biolab.isis.rl.ac.uk/uri/5dbhttp://biolab.isis.rl.ac.uk/camerons_labblog/12266/Transformation_of_pSOT092_and_pLLC146_into_BL21DE3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12266/Transformation_of_pSOT092_and_pLLC146_into_BL21DE3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12266/Transformation_of_pSOT092_and_pLLC146_into_BL21DE3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12266/Transformation_of_pSOT092_and_pLLC146_into_BL21DE3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12266/Transformation_of_pSOT092_and_pLLC146_into_BL21DE3.xml?revision=122661227212272GFP Lysis Buffer
Materials
cameronneylon.netCameron NeylonMaterial: Solution
20 mM Tris-HCl pH 7.7, 100 mM KCl, 8 mM imidazole
This Post is Linked By: GFP-First resin wash;Test purification of GFP;Purification of Sortase; ]]>2010-04-14T09:51:35+01:002010-04-14T09:51:35+01:00538b88fd9d465b1f4f5cb76fd8d816174Solutionhttp://biolab.isis.rl.ac.uk/uri/5e1http://biolab.isis.rl.ac.uk/camerons_labblog/12272/GFP_Lysis_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12272/GFP_Lysis_Buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12272/GFP_Lysis_Buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12272/GFP_Lysis_Buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12272/GFP_Lysis_Buffer.xml?revision=122721227512275GFP Cleared lysate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Cleared lysate from Test purification of GFP
This Post is Linked By: Test purification of GFP;GFP purification Gel; ]]>2010-04-14T11:56:22+01:002010-04-14T11:56:22+01:005d2bf8a00dcf867523049fe918d96f4fbSolutionhttp://biolab.isis.rl.ac.uk/uri/5e3http://biolab.isis.rl.ac.uk/camerons_labblog/12275/GFP_Cleared_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12275/GFP_Cleared_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12275/GFP_Cleared_lysate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12275/GFP_Cleared_lysate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12275/GFP_Cleared_lysate.xml?revision=122751227712277GFP purification first filtrate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
50 mL of filtrate from Test purification of GFP
This Post is Linked By: Test purification of GFP;GFP purification Gel; ]]>2010-04-14T12:12:13+01:002010-04-14T12:12:13+01:005ce171055267493003e372fa9e28f9334Solutionhttp://biolab.isis.rl.ac.uk/uri/5e4http://biolab.isis.rl.ac.uk/camerons_labblog/12277/GFP_purification_first_filtrate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12277/GFP_purification_first_filtrate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12277/GFP_purification_first_filtrate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12277/GFP_purification_first_filtrate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12277/GFP_purification_first_filtrate.xml?revision=122771227812278GFP purification second filtrate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Greenish murky filtrate solution from Test purification of GFP
This Post is Linked By: Test purification of GFP;GFP purification Gel; ]]>2010-04-14T12:13:17+01:002010-04-14T12:13:17+01:0052dd7a1702d081537357367f45a45c81bSolutionhttp://biolab.isis.rl.ac.uk/uri/5e5http://biolab.isis.rl.ac.uk/camerons_labblog/12278/GFP_purification_second_filtrate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12278/GFP_purification_second_filtrate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12278/GFP_purification_second_filtrate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12278/GFP_purification_second_filtrate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12278/GFP_purification_second_filtrate.xml?revision=122781228312283GFP-Second resin wash
Materials
cameronneylon.netCameron NeylonMaterial: Solution
100 mL flow though from second resin wash with Ammon-Acetate 20 mM imidazole wash buffer. Bright green!
This Post is Linked By: Test purification of GFP;Further purification of GFP fractions;GFP purifcation gel2; ]]>2010-04-14T12:32:38+01:002010-04-14T12:32:38+01:005c0504c1e628962225c8b088997afe0e3Solutionhttp://biolab.isis.rl.ac.uk/uri/5e9http://biolab.isis.rl.ac.uk/camerons_labblog/12283/GFPSecond_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12283/GFPSecond_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12283/GFPSecond_resin_wash.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12283/GFPSecond_resin_wash.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12283/GFPSecond_resin_wash.xml?revision=122831228112281Ammon-Acetate 20 mM imidazole wash buffer
Materials
cameronneylon.netCameron NeylonMaterial: Solution
20 mM ammonium acetate (pH 7)
20 mM imidazole
This Post is Linked By: GFP-Second resin wash;Test purification of GFP; ]]>2010-04-14T12:29:41+01:002010-04-14T12:29:41+01:0053fcdb0e20d6cb026cb847ba8f28da596Solutionhttp://biolab.isis.rl.ac.uk/uri/5e7http://biolab.isis.rl.ac.uk/camerons_labblog/12281/AmmonAcetate_20_mM_imidazole_wash_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12281/AmmonAcetate_20_mM_imidazole_wash_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12281/AmmonAcetate_20_mM_imidazole_wash_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12281/AmmonAcetate_20_mM_imidazole_wash_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12281/AmmonAcetate_20_mM_imidazole_wash_buffer.xml?revision=122811228012280GFP-First resin wash
Materials
cameronneylon.netCameron NeylonMaterial: Solution
About 100 mL of flow through from the washed resin from Test purification of GFP in GFP Lysis Buffer
This Post is Linked By: Test purification of GFP;GFP purification Gel;SDS-Page Gel; ]]>2010-04-14T12:20:06+01:002010-04-14T12:20:06+01:0052fb0d822c4efdc190edc914b702100dfSolutionhttp://biolab.isis.rl.ac.uk/uri/5e6http://biolab.isis.rl.ac.uk/camerons_labblog/12280/GFPFirst_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12280/GFPFirst_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12280/GFPFirst_resin_wash.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12280/GFPFirst_resin_wash.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12280/GFPFirst_resin_wash.xml?revision=122801228212285Ammonium-acetate 400 mM elution buffer
Materials
cameronneylon.netCameron NeylonMaterial: Solution
20 mM ammonium acetate pH 7
400 mM imidazole
This Post is Linked By: GFP-Third resin wash;Test purification of GFP; ]]>2010-04-14T12:30:56+01:002010-04-14T12:45:15+01:0052b752748fe21bf11de302b9170b5b93eSolutionhttp://biolab.isis.rl.ac.uk/uri/5e8http://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.xml?revision=12282http://biolab.isis.rl.ac.uk/camerons_labblog/12282/Ammoniumacetate_400_mM_elution_buffer.xml?revision=122851228612286GFP-Third resin wash
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Elution of remaining GFP from Test purification of GFP with Ammonium-acetate 20 mM elution buffer
This Post is Linked By: Test purification of GFP;Concentration and exchange of GFP; ]]>2010-04-14T12:46:16+01:002010-04-14T12:46:16+01:0058260f1f762391e490897f3e5ae531edeSolutionhttp://biolab.isis.rl.ac.uk/uri/5eahttp://biolab.isis.rl.ac.uk/camerons_labblog/12286/GFPThird_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12286/GFPThird_resin_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12286/GFPThird_resin_wash.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12286/GFPThird_resin_wash.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12286/GFPThird_resin_wash.xml?revision=122861227312292Test purification of GFP
Procedures
cameronneylon.netCameron NeylonProcedure: Protein_purification
Project: Protein-ligation
Pellet of GFP expressing cells (bright green, |10.5 g) was resuspended in GFP Lysis Buffer (50 mL) to give an even suspension. The suspended cells were then lysed by sonication (3 x 10 minutes, 30sec on, 30 sec off, on ice) and the lysate cleared by centrifugation (SS-34, 18krpm, 20 minutes, 4 C) to generate GFP Cleared lysate (50 mL).

Half the cleared lysate was resuspended with 5 mL of Ni-NTA resin which was bright green on settling but the solution remained quite green. Another 5 mL of resin was added and both combined and filtered, to yield a GFP purification first filtrate which was a dirty green/yellow. The remainder of the lysate was then added and filtered to generate a GFP purification second filtrate which was slightly brighter green than first but really a murky yellow.

The resin was then washed with 90 mL of GFP Lysis Buffer to giving GFP-First resin wash. The resin was then washed with about 100 mL of Ammon-Acetate 20 mM imidazole wash buffer to give GFP-Second resin washbut this was very green! Possibly a result of pH dropping low enough to protonate the histidines?

This solution was collected, buffer pH was about 6.6 so could well be protonating. The resin was then washed with about 20 mL of Ammonium-acetate 400 mM elution buffer and the eluant collected as GFP-Third resin wash.

The samples were run on an SDS-PAGE gel.
This Post is Linked By: GFP Cleared lysate;GFP purification first filtrate;GFP purification second filtrate;GFP-First resin wash;GFP-Third resin wash;Extraction; ]]>2010-04-14T10:15:06+01:002010-04-15T12:04:49+01:005a80c1ddc58eb5915bfa0c0beb65ad73bProtein_purificationProtein-ligationhttp://biolab.isis.rl.ac.uk/uri/5e2http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12273http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12274http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12276http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12279http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12284http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12287http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12288http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=12290http://biolab.isis.rl.ac.uk/camerons_labblog/12273/Test_purification_of_GFP.xml?revision=122921229812298GFP elution fraction 2
Materials
cameronneylon.netCameron NeylonMaterial: Solution
A 400 mM imidazole elution fraction from Further purification of GFP fractions


This Post is Linked By: Further purification of GFP fractions;GFP purifcation gel2;Concentration and exchange of GFP; ]]>2010-04-15T12:14:27+01:002010-04-15T12:14:27+01:005013b82387607ede7ba0a8a4c3c866b80Solutionhttp://biolab.isis.rl.ac.uk/uri/5f2http://biolab.isis.rl.ac.uk/camerons_labblog/12298/GFP_elution_fraction_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12298/GFP_elution_fraction_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12298/GFP_elution_fraction_2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12298/GFP_elution_fraction_2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12298/GFP_elution_fraction_2.xml?revision=122981229412294GFP-repurification flow through
Materials
cameronneylon.netCameron NeylonMaterial: Solution
First flow through from Further purification of GFP fractions
This Post is Linked By: Further purification of GFP fractions; ]]>2010-04-15T12:10:25+01:002010-04-15T12:10:25+01:0058d31e32eeacc64a5e1fc2987eab23555Solutionhttp://biolab.isis.rl.ac.uk/uri/5eehttp://biolab.isis.rl.ac.uk/camerons_labblog/12294/GFPrepurification_flow_through.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12294/GFPrepurification_flow_through.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12294/GFPrepurification_flow_through.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12294/GFPrepurification_flow_through.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12294/GFPrepurification_flow_through.xml?revision=122941229512295GFP-repurification first wash
Materials
cameronneylon.netCameron NeylonMaterial: Solution
20 mM imidazole wash from Further purification of GFP fractions
This Post is Linked By: Further purification of GFP fractions; ]]>2010-04-15T12:11:32+01:002010-04-15T12:11:32+01:00522a6f2f010c7944899aaa9f32c892e33Solutionhttp://biolab.isis.rl.ac.uk/uri/5efhttp://biolab.isis.rl.ac.uk/camerons_labblog/12295/GFPrepurification_first_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12295/GFPrepurification_first_wash.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12295/GFPrepurification_first_wash.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12295/GFPrepurification_first_wash.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12295/GFPrepurification_first_wash.xml?revision=122951229712297GFP elution fraction 1
Materials
cameronneylon.netCameron NeylonMaterial: Solution
A 400 mM imidazole elution fraction from Further purification of GFP fractions


This Post is Linked By: Further purification of GFP fractions;GFP purifcation gel2;Concentration and exchange of GFP; ]]>2010-04-15T12:13:52+01:002010-04-15T12:13:52+01:005b5a38b592e8861326a3eae98a3db6559Solutionhttp://biolab.isis.rl.ac.uk/uri/5f1http://biolab.isis.rl.ac.uk/camerons_labblog/12297/GFP_elution_fraction_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12297/GFP_elution_fraction_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12297/GFP_elution_fraction_1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12297/GFP_elution_fraction_1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12297/GFP_elution_fraction_1.xml?revision=122971229912299GFP elution fraction 3
Materials
cameronneylon.netCameron NeylonMaterial: Solution
A 400 mM imidazole elution fraction from Further purification of GFP fractions


This Post is Linked By: Further purification of GFP fractions;GFP purifcation gel2;Concentration and exchange of GFP; ]]>2010-04-15T12:14:31+01:002010-04-15T12:14:31+01:00590730a47f514faa3a4b60f06beb74c79Solutionhttp://biolab.isis.rl.ac.uk/uri/5f3http://biolab.isis.rl.ac.uk/camerons_labblog/12299/GFP_elution_fraction_3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12299/GFP_elution_fraction_3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12299/GFP_elution_fraction_3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12299/GFP_elution_fraction_3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12299/GFP_elution_fraction_3.xml?revision=122991230012300GFP elution fraction 4
Materials
cameronneylon.netCameron NeylonMaterial: Solution
A 400 mM imidazole elution fraction from Further purification of GFP fractions


This Post is Linked By: Further purification of GFP fractions;GFP purifcation gel2;Concentration and exchange of GFP; ]]>2010-04-15T12:14:38+01:002010-04-15T12:14:38+01:0059b5d6ee750b5341ca8f3ca820d3e58dcSolutionhttp://biolab.isis.rl.ac.uk/uri/5f4http://biolab.isis.rl.ac.uk/camerons_labblog/12300/GFP_elution_fraction_4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12300/GFP_elution_fraction_4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12300/GFP_elution_fraction_4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12300/GFP_elution_fraction_4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12300/GFP_elution_fraction_4.xml?revision=123001230112301GFP elution fraction 5
Materials
cameronneylon.netCameron NeylonMaterial: Solution
A 400 mM imidazole elution fraction from Further purification of GFP fractions


This Post is Linked By: Further purification of GFP fractions;Concentration and exchange of GFP; ]]>2010-04-15T12:14:50+01:002010-04-15T12:14:50+01:005aca057fba7c3fb46116802b604f34872Solutionhttp://biolab.isis.rl.ac.uk/uri/5f5http://biolab.isis.rl.ac.uk/camerons_labblog/12301/GFP_elution_fraction_5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12301/GFP_elution_fraction_5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12301/GFP_elution_fraction_5.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12301/GFP_elution_fraction_5.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12301/GFP_elution_fraction_5.xml?revision=123011229312302Further purification of GFP fractions
Procedures
cameronneylon.netCameron NeylonProcedure: Protein_purification
SDS-PAGE had showed that GFP-Second resin wash contained quite a lot of not very pure GFP. This was therefore attempted to be re-purified using the same resin. Firstly 5 mL of 1 M Tris-HCl pH 8 was added to the solution and the solution then flowed over the resin. Binding seemed poor so the flow through was re-diluted by half with Tris-HCl buffer and re-applied to the resin again. This appeared to bind much better.

The flow through was collected to give - GFP-repurification flow through
The resin was then washed with 20 mM imidazole in Tris-HCl buffer to give GFP-repurification first wash.

The resin was then washed with 400 mM imidazole in Tris-HCl buffer and 10-15 mL fractions collected to give.

GFP elution fraction 1
GFP elution fraction 2
GFP elution fraction 3
GFP elution fraction 4
GFP elution fraction 5
This Post is Linked By: GFP elution fraction 2;GFP-repurification flow through;GFP-repurification first wash;GFP elution fraction 1;GFP elution fraction;GFP elution fraction 3;GFP elution fraction 4;GFP elution fraction 5; ]]>2010-04-15T12:10:04+01:002010-04-15T12:15:27+01:0054c6770866e8e58009dd7d6a560b852daProtein_purificationhttp://biolab.isis.rl.ac.uk/uri/5edhttp://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.xml?revision=12293http://biolab.isis.rl.ac.uk/camerons_labblog/12293/Further_purification_of_GFP_fractions.xml?revision=123021230412304Sortase cleared lysate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The cleared lysate from Purification of Sortase
This Post is Linked By: Purification of Sortase; ]]>2010-04-15T12:27:23+01:002010-04-15T12:27:23+01:005c7cc34d10526c28a4b6fd54edf147133Solutionhttp://biolab.isis.rl.ac.uk/uri/5f7http://biolab.isis.rl.ac.uk/camerons_labblog/12304/Sortase_cleared_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12304/Sortase_cleared_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12304/Sortase_cleared_lysate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12304/Sortase_cleared_lysate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12304/Sortase_cleared_lysate.xml?revision=123041230812308Sortase flow through fraction
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The flow through fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T14:04:40+01:002010-04-15T14:04:40+01:005289107ff69b37d226b3ac0799d8c6db4Solutionhttp://biolab.isis.rl.ac.uk/uri/5f9http://biolab.isis.rl.ac.uk/camerons_labblog/12308/Sortase_flow_through_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12308/Sortase_flow_through_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12308/Sortase_flow_through_fraction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12308/Sortase_flow_through_fraction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12308/Sortase_flow_through_fraction.xml?revision=123081231412314Sortase elution fraction 4
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The fourth elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T15:13:53+01:002010-04-15T15:13:53+01:00512c0f5c4ab0db714df0aa3b0d2df5de1Solutionhttp://biolab.isis.rl.ac.uk/uri/5fehttp://biolab.isis.rl.ac.uk/camerons_labblog/12314/Sortase_elution_fraction_4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12314/Sortase_elution_fraction_4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12314/Sortase_elution_fraction_4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12314/Sortase_elution_fraction_4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12314/Sortase_elution_fraction_4.xml?revision=123141229612307fraction
Templates
cameronneylon.netCameron NeylonMaterials]] [[Material>Solution]]]]>Purification of Sortase

[[Section>Materials]]
[[Material>Solution]]
]]>2010-04-15T12:13:41+01:002010-04-15T14:04:02+01:0056a77a16765b43c72642eb73be306104bhttp://biolab.isis.rl.ac.uk/uri/5f0http://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.xml?revision=12296http://biolab.isis.rl.ac.uk/camerons_labblog/12296/fraction.xml?revision=123071230912309Sortase first wash fraction
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The 8 mM imidazole wash fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel; ]]>2010-04-15T14:06:13+01:002010-04-15T14:06:13+01:005aaa7af459b8df2f19366415024ae2a36Solutionhttp://biolab.isis.rl.ac.uk/uri/5fahttp://biolab.isis.rl.ac.uk/camerons_labblog/12309/Sortase_first_wash_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12309/Sortase_first_wash_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12309/Sortase_first_wash_fraction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12309/Sortase_first_wash_fraction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12309/Sortase_first_wash_fraction.xml?revision=123091231512315Sortase 20 mM imidazole wash fraction
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The 20 mM imidazole fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T15:15:53+01:002010-04-15T15:15:53+01:0055ce43e5e570809f1ada330c34edab084Solutionhttp://biolab.isis.rl.ac.uk/uri/5ffhttp://biolab.isis.rl.ac.uk/camerons_labblog/12315/Sortase_20_mM_imidazole_wash_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12315/Sortase_20_mM_imidazole_wash_fraction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12315/Sortase_20_mM_imidazole_wash_fraction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12315/Sortase_20_mM_imidazole_wash_fraction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12315/Sortase_20_mM_imidazole_wash_fraction.xml?revision=123151231212312Sortase elution fraction 2
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The second elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T15:13:24+01:002010-04-15T15:13:24+01:0058bf11e59319fd310b78eb66a3e47b166Solutionhttp://biolab.isis.rl.ac.uk/uri/5fchttp://biolab.isis.rl.ac.uk/camerons_labblog/12312/Sortase_elution_fraction_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12312/Sortase_elution_fraction_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12312/Sortase_elution_fraction_2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12312/Sortase_elution_fraction_2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12312/Sortase_elution_fraction_2.xml?revision=123121231312313Sortase elution fraction 3
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The third elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T15:13:38+01:002010-04-15T15:13:38+01:0055e2953a8912a5b6f4ac3760e5648d4a5Solutionhttp://biolab.isis.rl.ac.uk/uri/5fdhttp://biolab.isis.rl.ac.uk/camerons_labblog/12313/Sortase_elution_fraction_3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12313/Sortase_elution_fraction_3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12313/Sortase_elution_fraction_3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12313/Sortase_elution_fraction_3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12313/Sortase_elution_fraction_3.xml?revision=123131231112311Sortase elution fraction 1
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The first elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel;Concentration and exchange of sortase; ]]>2010-04-15T15:13:10+01:002010-04-15T15:13:10+01:005afa016c2bb811a5ccad0badd6079655dSolutionhttp://biolab.isis.rl.ac.uk/uri/5fbhttp://biolab.isis.rl.ac.uk/camerons_labblog/12311/Sortase_elution_fraction_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12311/Sortase_elution_fraction_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12311/Sortase_elution_fraction_1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12311/Sortase_elution_fraction_1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12311/Sortase_elution_fraction_1.xml?revision=123111232012320Sortase elution fraction 6
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The sixth elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel; ]]>2010-04-15T16:16:35+01:002010-04-15T16:16:35+01:0055213e85f99fe734ca316758445f885d0Solutionhttp://biolab.isis.rl.ac.uk/uri/601http://biolab.isis.rl.ac.uk/camerons_labblog/12320/Sortase_elution_fraction_6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12320/Sortase_elution_fraction_6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12320/Sortase_elution_fraction_6.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12320/Sortase_elution_fraction_6.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12320/Sortase_elution_fraction_6.xml?revision=123201232112321Sortase elution fraction 7
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The seventh elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;SDS-Page Gel; ]]>2010-04-15T16:16:52+01:002010-04-15T16:16:52+01:00560ab8ec21dd1acae5e5d5d00fddd7327Solutionhttp://biolab.isis.rl.ac.uk/uri/602http://biolab.isis.rl.ac.uk/camerons_labblog/12321/Sortase_elution_fraction_7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12321/Sortase_elution_fraction_7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12321/Sortase_elution_fraction_7.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12321/Sortase_elution_fraction_7.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12321/Sortase_elution_fraction_7.xml?revision=123211232212322Sortase elution fraction 8
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The 8th elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase;Sortase purification SDS-Page Gel; ]]>2010-04-15T16:17:36+01:002010-04-15T16:17:36+01:005d919a4c1b204cc7310ec7e21e4055208Solutionhttp://biolab.isis.rl.ac.uk/uri/603http://biolab.isis.rl.ac.uk/camerons_labblog/12322/Sortase_elution_fraction_8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12322/Sortase_elution_fraction_8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12322/Sortase_elution_fraction_8.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12322/Sortase_elution_fraction_8.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12322/Sortase_elution_fraction_8.xml?revision=123221232312323Sortase elution fraction 9
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The ninth elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase; ]]>2010-04-15T16:17:47+01:002010-04-15T16:17:47+01:005c06a6899492407a992db39ad2d79b654Solutionhttp://biolab.isis.rl.ac.uk/uri/604http://biolab.isis.rl.ac.uk/camerons_labblog/12323/Sortase_elution_fraction_9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12323/Sortase_elution_fraction_9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12323/Sortase_elution_fraction_9.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12323/Sortase_elution_fraction_9.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12323/Sortase_elution_fraction_9.xml?revision=123231232412324Sortase elution fraction 10
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The tenth elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase; ]]>2010-04-15T16:17:59+01:002010-04-15T16:17:59+01:005b52ea0e47d685c46434c9499cc7d154eSolutionhttp://biolab.isis.rl.ac.uk/uri/605http://biolab.isis.rl.ac.uk/camerons_labblog/12324/Sortase_elution_fraction_10.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12324/Sortase_elution_fraction_10.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12324/Sortase_elution_fraction_10.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12324/Sortase_elution_fraction_10.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12324/Sortase_elution_fraction_10.xml?revision=123241230312325Purification of Sortase
Procedures
cameronneylon.netCameron NeylonProcedure: Protein_purification
Six sortase stocks were grown up and induced (1L each). A gel of the six growths suggested good expression of Sortase in all cases. Pellet (21.1 g) was resuspended in around 105 mL of GFP Lysis Buffer. The suspensions were sonicated to lyse and then cleared by centrifugation (20 minutes, 18krpm, SS-34, 4 C) to give Sortase cleared lysate.

The lysate was poured over 10 mL of cleaned Ni-NTA Sepharose resin, to yield Sortase flow through fraction, a somewhat murky brown solution. The resin also turned an unpleasant brownish tinge. The resin was then washed with 100 mL of 20 mM Tris-HCl, 8 mM imidazole to yield Sortase first wash fraction. The resin was then washed with 20 mM imidazole buffer to yield Sortase 20 mM imidazole wash fraction.

The resin remained a dull blueish colour during this process. The resin was then washed with 20 mM Tris-HCl, 400 mM imidazole and 10-15 mL fractions collected to give: Sortase elution fraction 1, Sortase elution fraction 2, Sortase elution fraction 3, Sortase elution fraction 4, Sortase elution fraction 5, Sortase elution fraction 6, Sortase elution fraction 7, Sortase elution fraction 8, Sortase elution fraction 9, Sortase elution fraction 10.

As the resin was eluted it returned to a clearer blue colour. Quick bradford tests (but with old bradford reagent) showed the flow through to have a high protein concentration but the washes and the fractions to have relatively low concentration. Probably best to tell on a gel.
This Post is Linked By: Sortase cleared lysate;Sortase flow through fraction;Sortase elution fraction 4;GFP elution fraction;Sortase first wash fraction;Sortase 20 mM imidazole wash fraction;Sortase elution fraction 2;Sortase elution fraction 3;Sortase elution fraction 1;Sortase elution fraction 6;Sortase elution fraction 7;Sortase elution fraction 8;Sortase elution fraction 9;Sortase elution fraction 10;Sortase elution fraction 5; ]]>2010-04-15T12:27:10+01:002010-04-15T16:18:59+01:0057bd185e7f85db1874eb082c29feb301aProtein_purificationhttp://biolab.isis.rl.ac.uk/uri/5f6http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12303http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12305http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12310http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12316http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12317http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=12318http://biolab.isis.rl.ac.uk/camerons_labblog/12303/Purification_of_Sortase.xml?revision=123251231912319Sortase elution fraction 5
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The fifth elution fraction from Purification of Sortase


This Post is Linked By: Purification of Sortase; ]]>2010-04-15T16:16:23+01:002010-04-15T16:16:23+01:0050aac2ef3cee96d3ded8b0b7fd8e49f32Solutionhttp://biolab.isis.rl.ac.uk/uri/600http://biolab.isis.rl.ac.uk/camerons_labblog/12319/Sortase_elution_fraction_5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12319/Sortase_elution_fraction_5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12319/Sortase_elution_fraction_5.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12319/Sortase_elution_fraction_5.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12319/Sortase_elution_fraction_5.xml?revision=123191232812328Sigma Wide Range SDS-PAGE Marker
Materials
cameronneylon.netCameron NeylonMaterial: Solution
TODO: Link to webpage
This Post is Linked By: GFP purifcation gel2;GFP purification Gel;Sortase purification SDS-Page Gel; ]]>2010-04-16T09:18:33+01:002010-04-16T09:18:33+01:00577f7a02d410fff37f263650cb896807cSolutionhttp://biolab.isis.rl.ac.uk/uri/608http://biolab.isis.rl.ac.uk/camerons_labblog/12328/Sigma_Wide_Range_SDSPAGE_Marker.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12328/Sigma_Wide_Range_SDSPAGE_Marker.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12328/Sigma_Wide_Range_SDSPAGE_Marker.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12328/Sigma_Wide_Range_SDSPAGE_Marker.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12328/Sigma_Wide_Range_SDSPAGE_Marker.xml?revision=123281232712342SDS-Page Gel
Templates
cameronneylon.netCameron NeylonProcedures]] [[Procedure>SDS_PAGE]]]]>

LaneSampleul

1[[Material:Solution]][[box]]

2[[Material:Solution]][[box]]

3[[Material:Solution]][[box]]

4[[Material:Solution]][[box]]

5[[Material:Solution]][[box]]

6[[Material:Solution]][[box]]

7[[Material:Solution]][[box]]

8[[Material:Solution]][[box]]

9[[Material:Solution]][[box]]

10[[Material:Solution]][[box]]


A [[box]]% SDS-PAGE gel was polymerized according to Sambrook. Samples were loaded in gel loading buffer and the gel run at 180 V for approximately 1 hour.

[[Section>Procedures]]
[[Procedure>SDS_PAGE]]
]]>2010-04-16T09:17:38+01:002010-04-16T09:48:10+01:00563135382041f3411b6c91f8019df415dhttp://biolab.isis.rl.ac.uk/uri/607http://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.xml?revision=12327http://biolab.isis.rl.ac.uk/camerons_labblog/12327/SDSPage_Gel.xml?revision=123421233412339GFP purifcation gel2
Procedures
cameronneylon.netCameron NeylonProcedure: SDS_PAGE


Lane		Sampleul

1		Sigma Wide Range SDS-PAGE Marker5

2		GFP-Second resin wash5 fraction1

3		GFP-Second resin wash5 fraction2

4		GFP elution fraction 15

5		GFP elution fraction 25

6		GFP elution fraction 35

7		GFP elution fraction 45

8

9

10


A 12% SDS-PAGE gel was polymerized according to Sambrook. Samples were loaded in gel loading buffer and the gel run at 180 V for approximately 1 hour.

GFP second gel

This Post is Linked By: Concentration and exchange of GFP; ]]>2010-04-16T09:45:39+01:002010-04-16T09:47:29+01:005d65ad55f3270a7953b43db38fdd5f878SDS_PAGEhttp://biolab.isis.rl.ac.uk/data/1767.xmlhttp://biolab.isis.rl.ac.uk/uri/60bhttp://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xml?revision=12335http://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xml?revision=12336http://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xml?revision=12334http://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xml?revision=12338http://biolab.isis.rl.ac.uk/camerons_labblog/12334/GFP_purifcation_gel2.xml?revision=123391230612346Concentration and exchange of GFP
Procedures
cameronneylon.netCameron NeylonProcedure: Protein_purification
Project: Protein-ligation
On the basis of the gel GFP purifcation gel2, the following fractions: GFP elution fraction 1, GFP elution fraction 2, GFP elution fraction 3, GFP elution fraction 4, GFP elution fraction 5, and GFP-Third resin wash, total of about 100 mL were combined and concentrated by Amicon (10 kDa cutoff).

It was assumed that the repurification proceeded as did the first and these samples were reasonably pure. The resulting concentrated solution (about 30 mL) was dialyzed against 4 L of water overnight and then three further changes of 4 L prior to being frozen at -80 and freeze dried over the weekend to yield Freeze dried GFP.
This Post is Linked By: Freeze dried GFP; ]]>2010-04-15T14:01:06+01:002010-04-16T14:22:51+01:005c64c3289989093e08b5a20398fa17551Protein_purificationProtein-ligationhttp://biolab.isis.rl.ac.uk/uri/5f8http://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.xml?revision=12306http://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.xml?revision=12346http://biolab.isis.rl.ac.uk/camerons_labblog/12306/Concentration_and_exchange_of_GFP.xml?revision=123431235712357Frozen Sortase solution
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The frozen dialysate from Concentration and exchange of sortase
This Post is Linked By: Concentration and exchange of sortase; ]]>2010-04-21T09:30:53+01:002010-04-21T09:30:53+01:0059fca3009b4be81007276f327835fd87aSolutionhttp://biolab.isis.rl.ac.uk/uri/614http://biolab.isis.rl.ac.uk/camerons_labblog/12357/Frozen_Sortase_solution.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12357/Frozen_Sortase_solution.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12357/Frozen_Sortase_solution.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12357/Frozen_Sortase_solution.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12357/Frozen_Sortase_solution.xml?revision=123571233212340GFP purification Gel
Procedures
cameronneylon.netCameron NeylonProcedure: SDS_PAGE


Lane		Sampleul

1		Sigma Wide Range SDS-PAGE Marker5

2		GFP Cleared lysate5

3		GFP purification first filtrate5

4		GFP purification second filtrate5

5		GFP-First resin wash5 fraction1

6		GFP-First resin wash5 fraction2


A 12% SDS-PAGE gel was polymerized according to Sambrook. Samples were loaded in gel loading buffer and the gel run at 180 V for approximately 1 hour.

Essentially no useful bands visible on gel.
]]>2010-04-16T09:42:04+01:002010-04-16T09:47:41+01:005bd2b3c3adf26a4c1bfdf906db331031cSDS_PAGEhttp://biolab.isis.rl.ac.uk/uri/60ahttp://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.xml?revision=12333http://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.xml?revision=12332http://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.xml?revision=12337http://biolab.isis.rl.ac.uk/camerons_labblog/12332/GFP_purification_Gel.xml?revision=123401232912341Sortase purification SDS-Page Gel
Procedures
cameronneylon.netCameron NeylonProcedure: SDS_PAGE


Lane		Sampleul

1		Sigma Wide Range SDS-PAGE Marker5

2		Sortase flow through fraction5

3		Sortase first wash fraction5

4		Sortase 20 mM imidazole wash fraction5

5		Sortase elution fraction 15

6		Sortase elution fraction 25

7		Sortase elution fraction 35

8		Sortase elution fraction 45

9		Sortase elution fraction 65

10		Sortase elution fraction 85


A 12% SDS-PAGE gel was polymerized according to Sambrook. Samples were loaded in gel loading buffer and the gel run at 180 V for approximately 1 hour.

Sortase purification gel

This Post is Linked By: Concentration and exchange of sortase; ]]>2010-04-16T09:20:13+01:002010-04-16T09:47:54+01:005d5f1e1f179543021aa56b468f505a8c0SDS_PAGEhttp://biolab.isis.rl.ac.uk/data/1765.xmlhttp://biolab.isis.rl.ac.uk/uri/609http://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.xml?revision=12330http://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.xml?revision=12331http://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.xml?revision=12329http://biolab.isis.rl.ac.uk/camerons_labblog/12329/Sortase_purification_SDSPage_Gel.xml?revision=123411235812358Freeze dried Sortase
Materials
cameronneylon.netCameron NeylonMaterial: Powder
The freeze dried Sortase from Concentration and exchange of sortase.

Total weight of material: 141 mg


TubeEmpty weight (g)With protein (g)Protein (mg)
19.77399.801527.5
29.76289.791228.5
39.81499.844429.5
49.998410.027229
59.77449.801926.5

This Post is Linked By: Concentration and exchange of sortase;Ligation test of GFP and Fluor; ]]>2010-04-21T09:37:13+01:002010-04-21T09:37:13+01:00567cd72db897d33dae5cff3a7c3f68b92Powderhttp://biolab.isis.rl.ac.uk/uri/615http://biolab.isis.rl.ac.uk/camerons_labblog/12358/Freeze_dried_Sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12358/Freeze_dried_Sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12358/Freeze_dried_Sortase.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12358/Freeze_dried_Sortase.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12358/Freeze_dried_Sortase.xml?revision=123581234512356Freeze dried GFP
Materials
cameronneylon.netCameron NeylonMaterial: Powder
The final product of freeze dried purified GFP from Concentration and exchange of GFP.

Total weight of material: 141 mg


TubeEmpty weight (g)With protein (g)Protein (mg)
19.80309.862960
29.80229.883181


I may have mixed up the lids for the two tubes but the total weight should be correct regardless.
This Post is Linked By: Concentration and exchange of GFP;Ligation test of GFP and Fluor; ]]>2010-04-16T14:22:39+01:002010-04-21T09:29:17+01:0052847e49fed9728f42615085065e54435Powderhttp://biolab.isis.rl.ac.uk/uri/60dhttp://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.xml?revision=12345http://biolab.isis.rl.ac.uk/camerons_labblog/12345/Freeze_dried_GFP.xml?revision=123561234412359Concentration and exchange of sortase
Procedures
cameronneylon.netCameron NeylonProcedure: Protein_purification
Project: Protein-ligation
On the basis of the gel Sortase purification SDS-Page Gel the solution from Sortase flow through fraction was repurified as before and equivalent fractions were combined. The Sortase 20 mM imidazole wash fraction, around 200 mL was combined and concentrated to around 30 mL at which point it had started to precipitate and then dialyzed against 4L of water over the weekend at 4C.

The fractions Sortase elution fraction 1, Sortase elution fraction 2, Sortase elution fraction 3, Sortase elution fraction 4 and the equivalent from the repurified sample were combined and concentrated to a total of around 80 mL and dialyzed against 4 L of water over the weekend at 4C.

One dialysis tube (about 30 mL) was taken and the solution frozen directly at -80 C to give Frozen Sortase solution. The remainder was combined and freeze dried to give Freeze dried Sortase
This Post is Linked By: Frozen Sortase solution;Freeze dried Sortase; ]]>2010-04-16T09:52:40+01:002010-04-21T09:40:23+01:0053acf3f3eb84eb482d093f4f68fc09e26Protein_purificationProtein-ligationhttp://biolab.isis.rl.ac.uk/uri/60chttp://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.xml?revision=12344http://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.xml?revision=12347http://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.xml?revision=12348http://biolab.isis.rl.ac.uk/camerons_labblog/12344/Concentration_and_exchange_of_sortase.xml?revision=123591213112745SANS2d run template
Templates
cameronneylon.netCameron NeylonProcedures]] [[Procedure>SANS]] [[Instrument>SANS2d]] [[Project>Glur0]]]]>

SampleSANS/TRANSExposure time (uamp.hr)Run #Data
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]
[[Material:Solution]][[box=2]][[Box=5]][[Box=5]][[Blog]]



[[Section>Procedures]]
[[Procedure>SANS]]
[[Instrument>SANS2d]]
[[Project>Glur0]]
]]>2010-03-09T19:49:06+00:002010-06-22T15:08:11+01:005bbecfddfa74344af388ad8aec763bff0http://biolab.isis.rl.ac.uk/uri/593http://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.xml?revision=12131http://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.xml?revision=12136http://biolab.isis.rl.ac.uk/camerons_labblog/12131/SANS2d_run_template.xml?revision=127451213712748First Glur0 runs - SANS2d
Procedures
cameronneylon.netCameron NeylonProcedure: SANS
Instrument: SANS2d
Project: Glur0
The following samples were run as described on SANS2d at ISIS. The samples were run in 1 mm path length rectangular cells (200 uL samples) at a temperature of 20 C. The run script is attached.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
D2O Buffer for Glur0S403325
Empty beamS153326
Glur0 D2O SampleT63328
Glur0 H2O SampleT63329
H2O Buffer for Glur0T63330
D2O Buffer for Glur0T63331
empty beamS83332
Glur0 D2O SampleS403333
Glur0 H2O SampleS403334


GCL script

]]>2010-03-09T22:28:27+00:002010-06-23T09:51:01+01:0057a522b7d4fef3750a9dc6dbd8f259721SANSSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1707.xmlhttp://biolab.isis.rl.ac.uk/uri/598http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=12138http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=12137http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=12147http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=12146http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=12149http://biolab.isis.rl.ac.uk/camerons_labblog/12137/First_Glur0_runs__SANS2d.xml?revision=127481315013155Purification of Tus-Ter complex
Procedures
cameronneylon.netCameron NeylonProcedure: Sample_Preparation
Jon Burns mixed Tus (about 30 uM, 200 uL) with 21-mer Ter DNA and 500 uL of the resultant solution was run on the S75 column yielding a large number of fractions. The trace showed some aggregate eluting in the void volume (about 20 mL) and some absorbances at 260. The apparent protein peak (complex) eluted at around 48 mL which is earlier than that from the protein alone on this column. There were three presumed DNA peaks which is worrying. Running the DNA alone replicated those three peaks providing confidence that the new peak with a strong 280 nm absorbance was the complex.

Fractions D6 to E3 were combined and concentrated to
]]>2010-08-12T16:36:54+01:002010-08-12T16:53:35+01:005f16466dc9f31444358c77715b2c56b58Sample_Preparationhttp://biolab.isis.rl.ac.uk/data/2121.xmlhttp://biolab.isis.rl.ac.uk/data/2123.xmlhttp://biolab.isis.rl.ac.uk/uri/72ahttp://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=13150http://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=13151http://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=13152http://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=13153http://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=13154http://biolab.isis.rl.ac.uk/camerons_labblog/13150/Purification_of_TusTer_complex.xml?revision=131551315613167Purification of Tus-Ter(14mer) complex, Tus-NS(21mer), Tus-Ter(Ext)
Procedures
cameronneylon.netCameron NeylonProcedure: Sample_Preparation
Sample of Ter DNA (14 mer from Kamada paper) was mixed with 297 uL of Tus sample, incubated at RT for 10 minutes and then loaded onto the S75.

There seem to be two relevant peaks, one at 49 mL and one at 62. Will concentrate 62 mL peak (E10-F8) but this may be free protein.

Sample of NS DNA (21 mer from biochemistry paper) was mixed with 297 uL of Tus sample, incubated at RT for 10 minutes and then loaded onto the S75. Main protein peak at 62 minutes, concentrate fraction E9-F9.

Sample of Ter DNA (Jon's extended sequence) was mixed with 297 uL of Tus sample, incubated at RT for 10 minutes and then loaded onto the S75.

Trace is a bit of a mess. Can't see a clear protein peak and seem to have three DNA peaks. Not sure what to do with this.

Sample of NS DNA (extended) was mixed with 297 uL of Tus sample, incubated at RT for 10 minutes and then loaded onto the S75. Main protein peak at 62 minutes, concentrate fraction E9-F9.

Again, as with the Ter case the trace is not convincing. The peak at 55-59 mL was selected and concentrated.
This Post is Linked By: Concentrated sample from Tus-NS(21mer) S75 column;Concentrated sample from Tus-Ter(14mer) S75 column; ]]>2010-08-12T21:48:55+01:002010-08-13T10:47:19+01:00599aabb2527785ea1f185bafae79634c9Sample_Preparationhttp://biolab.isis.rl.ac.uk/data/2126.xmlhttp://biolab.isis.rl.ac.uk/data/2128.xmlhttp://biolab.isis.rl.ac.uk/data/2130.xmlhttp://biolab.isis.rl.ac.uk/data/2132.xmlhttp://biolab.isis.rl.ac.uk/uri/72bhttp://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13156http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13159http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13160http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13161http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13163http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13164http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13165http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=13166http://biolab.isis.rl.ac.uk/camerons_labblog/13156/Purification_of_TusTer14mer_complex_TusNS21mer_TusTerExt.xml?revision=131671316913169Concentrated sample from Tus-NS(21mer) S75 column
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Sample from Purification of Tus-Ter(14mer) complex, Tus-NS(21mer), Tus-Ter(Ext)
]]>2010-08-13T11:12:19+01:002010-08-13T11:12:19+01:0055f2ae3d7c49ab7aebb3065f693aacfbdSolutionhttp://biolab.isis.rl.ac.uk/uri/72ehttp://biolab.isis.rl.ac.uk/camerons_labblog/13169/Concentrated_sample_from_TusNS21mer_S75_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13169/Concentrated_sample_from_TusNS21mer_S75_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13169/Concentrated_sample_from_TusNS21mer_S75_column.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13169/Concentrated_sample_from_TusNS21mer_S75_column.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13169/Concentrated_sample_from_TusNS21mer_S75_column.xml?revision=131691316813168Concentrated sample from Tus-Ter(14mer) S75 column
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Sample from Purification of Tus-Ter(14mer) complex, Tus-NS(21mer), Tus-Ter(Ext)
]]>2010-08-13T11:11:18+01:002010-08-13T11:11:18+01:0059e59af4bf933746d8682cef77716622bSolutionhttp://biolab.isis.rl.ac.uk/uri/72dhttp://biolab.isis.rl.ac.uk/camerons_labblog/13168/Concentrated_sample_from_TusTer14mer_S75_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13168/Concentrated_sample_from_TusTer14mer_S75_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13168/Concentrated_sample_from_TusTer14mer_S75_column.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13168/Concentrated_sample_from_TusTer14mer_S75_column.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13168/Concentrated_sample_from_TusTer14mer_S75_column.xml?revision=1316813249132493339 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3339



This Post is Linked By: SANS Data Reduction; ]]>2010-09-13T11:51:36+01:002010-09-13T11:51:36+01:0051835bb91fe343779e26bfce378745d07SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2171.xmlhttp://biolab.isis.rl.ac.uk/uri/750http://biolab.isis.rl.ac.uk/camerons_labblog/13249/3339__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13249/3339__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13249/3339__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13249/3339__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13249/3339__reduced_SANS_data.xml?revision=1324913250132503395 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3395



This Post is Linked By: SANS Data Reduction; ]]>2010-09-13T11:52:12+01:002010-09-13T11:52:12+01:00542577b410daaa586850ef2e14567e776SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2173.xmlhttp://biolab.isis.rl.ac.uk/uri/751http://biolab.isis.rl.ac.uk/camerons_labblog/13250/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13250/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13250/3395__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13250/3395__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13250/3395__reduced_SANS_data.xml?revision=1325013251132513397 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3397



This Post is Linked By: SANS Data Reduction; ]]>2010-09-13T11:52:50+01:002010-09-13T11:52:50+01:005eb98dca70f18eef1edf6c13d044f32fcSANS2DSANShttp://biolab.isis.rl.ac.uk/data/2175.xmlhttp://biolab.isis.rl.ac.uk/uri/752http://biolab.isis.rl.ac.uk/camerons_labblog/13251/3397__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13251/3397__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13251/3397__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13251/3397__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13251/3397__reduced_SANS_data.xml?revision=1325113252132523393 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3393



This Post is Linked By: SANS Data Reduction; ]]>2010-09-13T11:53:30+01:002010-09-13T11:53:30+01:005ad4f4b207e9d7ede77d49abd782bebd0SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2177.xmlhttp://biolab.isis.rl.ac.uk/uri/753http://biolab.isis.rl.ac.uk/camerons_labblog/13252/3393__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13252/3393__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13252/3393__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13252/3393__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13252/3393__reduced_SANS_data.xml?revision=1325213253132533345 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3345



This Post is Linked By: SANS Data Reduction; ]]>2010-09-13T11:55:08+01:002010-09-13T11:55:08+01:005ecb22fda72ed716beedf706daa1eeb6fSANS2DSANShttp://biolab.isis.rl.ac.uk/data/2179.xmlhttp://biolab.isis.rl.ac.uk/uri/754http://biolab.isis.rl.ac.uk/camerons_labblog/13253/3345__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13253/3345__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13253/3345__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13253/3345__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13253/3345__reduced_SANS_data.xml?revision=132531325413254SANS Data Reduction
Procedures
cameronneylon.netCameron NeylonProcedure: Data_reduction
SANS RunSANS TransBgd RunBgd TransReduced data
33393328334233313339 - reduced SANS data
33953355339633563395 - reduced SANS data
33973353339233543397 - reduced SANS data
33933346339433473393 - reduced SANS data
33453329334133303345 - reduced SANS data


]]>2010-09-13T11:55:08+01:002010-09-13T11:55:08+01:0059b78e9229129597c7f53cfd10ca601aeData_reductionhttp://biolab.isis.rl.ac.uk/uri/755http://biolab.isis.rl.ac.uk/camerons_labblog/13254/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13254/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13254/SANS_Data_Reduction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13254/SANS_Data_Reduction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13254/SANS_Data_Reduction.xml?revision=1325413323133233361 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3361



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:40:03+01:002010-09-23T10:40:03+01:0059216513a83527f30cce6dfbe533d3ed8SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2199.xmlhttp://biolab.isis.rl.ac.uk/uri/768http://biolab.isis.rl.ac.uk/camerons_labblog/13323/3361__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13323/3361__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13323/3361__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13323/3361__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13323/3361__reduced_SANS_data.xml?revision=1332313324133243367 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3367



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:40:31+01:002010-09-23T10:40:31+01:005ca0e0ae7197049d26f28855a47b562dcSANS2DSANShttp://biolab.isis.rl.ac.uk/data/2201.xmlhttp://biolab.isis.rl.ac.uk/uri/769http://biolab.isis.rl.ac.uk/camerons_labblog/13324/3367__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13324/3367__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13324/3367__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13324/3367__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13324/3367__reduced_SANS_data.xml?revision=1332413325133253377 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3377



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:40:58+01:002010-09-23T10:40:58+01:00565b3098239ee06639e3d07b12ec1efe2SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2203.xmlhttp://biolab.isis.rl.ac.uk/uri/76ahttp://biolab.isis.rl.ac.uk/camerons_labblog/13325/3377__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13325/3377__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13325/3377__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13325/3377__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13325/3377__reduced_SANS_data.xml?revision=1332513326133263386 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3386



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:41:27+01:002010-09-23T10:41:27+01:0050850a3eccbfa062529d019b58fc990c2SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2205.xmlhttp://biolab.isis.rl.ac.uk/uri/76bhttp://biolab.isis.rl.ac.uk/camerons_labblog/13326/3386__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13326/3386__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13326/3386__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13326/3386__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13326/3386__reduced_SANS_data.xml?revision=1332613327133273395 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3395



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:41:57+01:002010-09-23T10:41:57+01:005771cffb25ca23885f7f61007511798d4SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2207.xmlhttp://biolab.isis.rl.ac.uk/uri/76chttp://biolab.isis.rl.ac.uk/camerons_labblog/13327/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13327/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13327/3395__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13327/3395__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13327/3395__reduced_SANS_data.xml?revision=133271332813331SANS Data Reduction
Procedure
cameronneylon.netCameron NeylonProcedure: Data_reduction
I've reduced each of the 80% D2O runs individually to check whether there is something wrong with one of them as the background subtraction for the whole set gives strange results.

SANS RunSANS TransBgd RunBgd TransReduced data
33613355336233563361 - reduced SANS data
33673355336233563367 - reduced SANS data
33773355336233563377 - reduced SANS data
33863355336233563386 - reduced SANS data
33953355336233563395 - reduced SANS data


Based on the graph there doesn't seem much difference, although there is some evidence of aggregation for the later samples. Next job is to check the backgrounds for variation.
Comparison graph

]]>2010-09-23T10:41:58+01:002010-09-23T10:47:45+01:0054cc59a5e57684cfedef39980e4c0dfc7Data_reductionhttp://biolab.isis.rl.ac.uk/data/2209.xmlhttp://biolab.isis.rl.ac.uk/uri/76dhttp://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.xml?revision=13328http://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.xml?revision=13330http://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.xml?revision=13331http://biolab.isis.rl.ac.uk/camerons_labblog/13328/SANS_Data_Reduction.xml?revision=1332913332133323362 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3362



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:55:45+01:002010-09-23T10:55:45+01:0055635ffaae6a49b70f453c2fbe8e88ee6SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2211.xmlhttp://biolab.isis.rl.ac.uk/uri/76ehttp://biolab.isis.rl.ac.uk/camerons_labblog/13332/3362__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13332/3362__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13332/3362__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13332/3362__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13332/3362__reduced_SANS_data.xml?revision=1333213333133333368 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3368



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:56:19+01:002010-09-23T10:56:19+01:00526d58b016cad6fa204244d7f2fb1d391SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2213.xmlhttp://biolab.isis.rl.ac.uk/uri/76fhttp://biolab.isis.rl.ac.uk/camerons_labblog/13333/3368__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13333/3368__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13333/3368__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13333/3368__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13333/3368__reduced_SANS_data.xml?revision=1333313334133343378 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3378



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:56:51+01:002010-09-23T10:56:51+01:0051906f724ccf2371693d5663523262d85SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2215.xmlhttp://biolab.isis.rl.ac.uk/uri/770http://biolab.isis.rl.ac.uk/camerons_labblog/13334/3378__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13334/3378__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13334/3378__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13334/3378__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13334/3378__reduced_SANS_data.xml?revision=1333413335133353383 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3383



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:57:19+01:002010-09-23T10:57:19+01:00538cb0d5797f91b1faa6394ef070b8e08SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2217.xmlhttp://biolab.isis.rl.ac.uk/uri/771http://biolab.isis.rl.ac.uk/camerons_labblog/13335/3383__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13335/3383__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13335/3383__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13335/3383__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13335/3383__reduced_SANS_data.xml?revision=1333513336133363387 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3387



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:57:49+01:002010-09-23T10:57:49+01:0053c54619d975d128b82c490b8c6af3360SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2219.xmlhttp://biolab.isis.rl.ac.uk/uri/772http://biolab.isis.rl.ac.uk/camerons_labblog/13336/3387__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13336/3387__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13336/3387__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13336/3387__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13336/3387__reduced_SANS_data.xml?revision=1333613337133373396 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3396



This Post is Linked By: SANS Data Reduction; ]]>2010-09-23T10:58:18+01:002010-09-23T10:58:18+01:00535484a547f501a44c5773b9418673b99SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2221.xmlhttp://biolab.isis.rl.ac.uk/uri/773http://biolab.isis.rl.ac.uk/camerons_labblog/13337/3396__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13337/3396__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13337/3396__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13337/3396__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13337/3396__reduced_SANS_data.xml?revision=133371333813341SANS Data Reduction
Procedure
cameronneylon.netCameron NeylonProcedure: Data_reduction
As the sample runs looked very similar for 80% D2O samples am now checking the buffers to see whether one of them is out of wack.

SANS RunSANS TransBgd RunBgd TransReduced data
33623356334933483362 - reduced SANS data
33683356334933483368 - reduced SANS data
33783356334933483378 - reduced SANS data
33833356334933483383 - reduced SANS data
33873356334933483387 - reduced SANS data
33963356334933483396 - reduced SANS data


Comparing the buffers (using D2O as the background) clearly 3383 is somehow out of wack. Possibly just not labelled correctly, looks suspiciously like a water background.

Buffer scattering comparison


Will need to re-do the add file for 80% D2O background and then re-do the reductions.
]]>2010-09-23T10:58:19+01:002010-09-23T11:03:14+01:005815005cf64a1489b2e8d73b4781d7048Data_reductionhttp://biolab.isis.rl.ac.uk/data/2223.xmlhttp://biolab.isis.rl.ac.uk/uri/774http://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.xml?revision=13338http://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.xml?revision=13340http://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.xml?revision=13339http://biolab.isis.rl.ac.uk/camerons_labblog/13338/SANS_Data_Reduction.xml?revision=133411324713342Adding runs from March GLur0 SANS Expt
Procedures
cameronneylon.netCameron NeylonProcedure: SANS
Instrument: SANS2d
A bunch of runs weren't explicitly recorded for this experiment so I am recording the complete set of runs here.


SampleRunsTransmission
D2O33493373338233913348
Glur0 H2O Sample3334334033453329
Glur0 in 22% D2O buffer3350335933653375338433933346
Glur0-42% D2O33573363336933703379338833973353
Glur0-80% D2O336133673377338633953355
Glur0 D2O Sample333333393328
10mM DM H2O Buffer333633413330
10 mM DM 22%D2O buffer3351336033663376338533943347
10 mM DM 40%D2O buffer335833643370337433923354
10 mM DM 80%D2O buffer336233683378338733963356
10mM DM D2O Buffer3325333733423331


Empty beam transmission: 3332

Run 3383 does not appear to be an 80% D2O run but probably water. This run was taken out of subsequently added sets.
]]>2010-09-13T09:21:20+01:002010-09-23T11:12:08+01:005ca922cf9a10324b0a505dc0173729e76SANSSANS2dhttp://biolab.isis.rl.ac.uk/uri/74fhttp://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.xml?revision=13248http://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.xml?revision=13247http://biolab.isis.rl.ac.uk/camerons_labblog/13247/Adding_runs_from_March_GLur0_SANS_Expt.xml?revision=1334213346133463345 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3345



This Post is Linked By: SANS Data Reduction;Guinier and matchpoint analysis of GluR0 SANS data; ]]>2010-09-23T11:33:25+01:002010-09-23T11:33:25+01:0053a4e118d78871b382dd0b08a4e81be84SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2233.xmlhttp://biolab.isis.rl.ac.uk/uri/778http://biolab.isis.rl.ac.uk/camerons_labblog/13346/3345__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13346/3345__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13346/3345__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13346/3345__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13346/3345__reduced_SANS_data.xml?revision=1334613347133473393 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3393



This Post is Linked By: SANS Data Reduction;Guinier and matchpoint analysis of GluR0 SANS data; ]]>2010-09-23T11:34:05+01:002010-09-23T11:34:05+01:0056323a00d6caa67058cae6f09a799c1b5SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2235.xmlhttp://biolab.isis.rl.ac.uk/uri/779http://biolab.isis.rl.ac.uk/camerons_labblog/13347/3393__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13347/3393__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13347/3393__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13347/3393__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13347/3393__reduced_SANS_data.xml?revision=1334713348133483397 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3397



This Post is Linked By: SANS Data Reduction;Guinier and matchpoint analysis of GluR0 SANS data; ]]>2010-09-23T11:34:46+01:002010-09-23T11:34:46+01:00526bfaa1e2ea8fb9f787ee17452a0d873SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2237.xmlhttp://biolab.isis.rl.ac.uk/uri/77ahttp://biolab.isis.rl.ac.uk/camerons_labblog/13348/3397__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13348/3397__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13348/3397__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13348/3397__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13348/3397__reduced_SANS_data.xml?revision=1334813349133493395 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3395



This Post is Linked By: SANS Data Reduction;Guinier and matchpoint analysis of GluR0 SANS data; ]]>2010-09-23T11:35:36+01:002010-09-23T11:35:36+01:005e9fb917fcf0ac9cc94e92160f79ed89dSANS2DSANShttp://biolab.isis.rl.ac.uk/data/2239.xmlhttp://biolab.isis.rl.ac.uk/uri/77bhttp://biolab.isis.rl.ac.uk/camerons_labblog/13349/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13349/3395__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13349/3395__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13349/3395__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13349/3395__reduced_SANS_data.xml?revision=1334913350133503339 - reduced SANS data
Data
cameronneylon.netCameron NeylonInstrument: SANS2D
Data Type: SANS
Reduced SANS Data

3339



This Post is Linked By: SANS Data Reduction;Guinier and matchpoint analysis of GluR0 SANS data; ]]>2010-09-23T11:36:16+01:002010-09-23T11:36:16+01:005103f31a891d88fc86f421a7501cf80e5SANS2DSANShttp://biolab.isis.rl.ac.uk/data/2241.xmlhttp://biolab.isis.rl.ac.uk/uri/77chttp://biolab.isis.rl.ac.uk/camerons_labblog/13350/3339__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13350/3339__reduced_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13350/3339__reduced_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13350/3339__reduced_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13350/3339__reduced_SANS_data.xml?revision=133501335113355SANS Data Reduction
Procedure
cameronneylon.netCameron NeylonProcedure: Data_reduction
Reduced data for the contrast series of Glur0 in DM micelles from D2O to H2O. Samples are 100% D2O, 80% D2O, 42% D2O, 20% D2O, and H2O in that order.

SANS RunSANS TransBgd RunBgd TransReduced data
33453329334133303345 - reduced SANS data
33933346339433473393 - reduced SANS data
33973353339233543397 - reduced SANS data
33953355339633563395 - reduced SANS data
33393328334233313339 - reduced SANS data


Contrast series graph


Initial graph. The later runs show some aggregation based on the other examples so should possibly look to compare those and see which parts of the curve are reliable compared to others.a
]]>2010-09-23T11:36:17+01:002010-09-23T11:42:45+01:00521891e621d350c08f4c0ffcb29fff850Data_reductionhttp://biolab.isis.rl.ac.uk/data/2243.xmlhttp://biolab.isis.rl.ac.uk/uri/77dhttp://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xml?revision=13351http://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xml?revision=13353http://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xml?revision=13352http://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xml?revision=13355http://biolab.isis.rl.ac.uk/camerons_labblog/13351/SANS_Data_Reduction.xml?revision=133541361813626Guinier and matchpoint analysis of GluR0 SANS data
Procedure
cameronneylon.netCameron Neylon-1 and the fits obtained compared to those obtained using the Guinier-Porod model in the NCNR Igor Pro analysis suite. The data from the 20% and 42% ([blog]13348[/blog] and [blog]13347[/blog] respectively) are too noisy to provide good estimates of Rg or I(0). [table] [row]Sample[col]Input Data[col]Rg[col]Scale - I(0)[col]Fit location[/row] [row]100% D2O[col][blog]13350[/blog][col]139 +/- 12[col]204 +/- 15[col][url=http://www.sansanalysis.com/analysis/share/fit/73d17037aa55c2c07bd08420242f84f7/]Online fit[/url][/row] [row]80% D2O[col][blog]13349[/blog][col]149 +/- 9.3[col]111 +/- 7.2[col][url=http://www.sansanalysis.com/analysis/share/fit/8114a68c4fdce74c055b68130e2bd7d2/]Online fit[/url][/row] [row]H2O[col][blog]13346[/blog][col]148 +/- 39[col]63.2 +/- 17[col][url=http://www.sansanalysis.com/analysis/share/fit/d59863cde2beb7ab593f21196caea07a/]Online fit[/url][/row] [/table] The Guinier fits provide an approximately consistent Rg of around 140-150 A which seems a reasonable starting point. The scale factors are changing more or less as expected given the assumption that the detergent makes a realtively small contribution to the overall scattering. Assuming scattering was due to protein only and therefore the match point is ~40% and scaling linearly an I(0) of 200 cm-1 in D2O would be expected to give I(0) of ~90 in 80% D2O and of ~80 in H2O. This underestimates the scattering in 80% D2O where the detergent would be expected to contribute significantly and overestimates for H2O where the detergent is nearly matched. The actual matchpoint calculated from graphing sqrt[I(0)] vs % D2O is ~35% D2O. A quadratic fit gives match point of 32% [data]2294[/data] According to ChemSpider ([url=http://www.chemspider.com/Chemical-Structure.4450827.html]CID:4450827[/url]) the density of decyl maltoside (predicted) is 1.32 g/mL. From the [url=http://www.ncnr.nist.gov/resources/sldcalc.html]NCNR SLD calculator[/url] the SLD of H-DM is -0.75E-6 A^-2 and of D-DM is 1.93E-6 A^-2. The protein SLD goes from 1.8 - 3.1 (based on ordinary protein, not specifically calculated). According to calculation this gives a volume fraction of protein in the aggregate of 87%. Error bars on that are probably significant. So somewhere around 85-90% by volume would be reasonable.]]>Procedure: Data_Analysis
Project: Glur0
The data that were recently reduced were submitted in the first instance for Guinier fitting at sansanalysis.com The data were fitted from Q = 0.045 - 0.01 A-1 and the fits obtained compared to those obtained using the Guinier-Porod model in the NCNR Igor Pro analysis suite. The data from the 20% and 42% (3397 - reduced SANS data and 3393 - reduced SANS data respectively) are too noisy to provide good estimates of Rg or I(0).


SampleInput DataRgScale - I(0)Fit location
100% D2O3339 - reduced SANS data139 +/- 12204 +/- 15Online fit
80% D2O3395 - reduced SANS data149 +/- 9.3111 +/- 7.2Online fit
H2O3345 - reduced SANS data148 +/- 3963.2 +/- 17Online fit


The Guinier fits provide an approximately consistent Rg of around 140-150 A which seems a reasonable starting point. The scale factors are changing more or less as expected given the assumption that the detergent makes a realtively small contribution to the overall scattering. Assuming scattering was due to protein only and therefore the match point is ~40% and scaling linearly an I(0) of 200 cm-1 in D2O would be expected to give I(0) of ~90 in 80% D2O and of ~80 in H2O. This underestimates the scattering in 80% D2O where the detergent would be expected to contribute significantly and overestimates for H2O where the detergent is nearly matched.

The actual matchpoint calculated from graphing sqrt[I(0)] vs % D2O is ~35% D2O. A quadratic fit gives match point of 32%

Match point determination


According to ChemSpider (CID:4450827) the density of decyl maltoside (predicted) is 1.32 g/mL. From the NCNR SLD calculator the SLD of H-DM is -0.75E-6 A^-2 and of D-DM is 1.93E-6 A^-2. The protein SLD goes from 1.8 - 3.1 (based on ordinary protein, not specifically calculated).

According to calculation this gives a volume fraction of protein in the aggregate of 87%. Error bars on that are probably significant. So somewhere around 85-90% by volume would be reasonable.
]]>2010-10-04T11:58:18+01:002010-10-05T10:18:24+01:005c22a81c073114534eb2c5ee15bb5fc8fData_AnalysisGlur0http://biolab.isis.rl.ac.uk/data/2294.xmlhttp://biolab.isis.rl.ac.uk/uri/78fhttp://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13618http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13620http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13619http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13621http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13622http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13623http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=13624http://biolab.isis.rl.ac.uk/camerons_labblog/13618/Guinier_and_matchpoint_analysis_of_GluR0_SANS_data.xml?revision=136261370113701Cleared GluR0 lysate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
The cleared lysate from
This Post is Linked By: GluR0 First Purification SDS-Page Gel;Glur0 purification; ]]>2010-10-20T12:57:45+01:002010-10-20T12:57:45+01:005d3c947c742e66b5f86867c5d4490e202SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7a0http://biolab.isis.rl.ac.uk/camerons_labblog/13701/Cleared_GluR0_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13701/Cleared_GluR0_lysate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13701/Cleared_GluR0_lysate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13701/Cleared_GluR0_lysate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13701/Cleared_GluR0_lysate.xml?revision=137011370313706Fractions C1/C2 from Glur0 Cobalt column
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
The two fractions containing Glur0 eluted from the cobalt column in Glur0 purification
This Post is Linked By: GluR0 First Purification SDS-Page Gel;Glur0 purification; ]]>2010-10-20T13:20:12+01:002010-10-20T13:30:47+01:005345082243eaaab47b97d995343dd3fedSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7a2http://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.xml?revision=13703http://biolab.isis.rl.ac.uk/camerons_labblog/13703/Fractions_C1C2_from_Glur0_Cobalt_column.xml?revision=137061372313723Concentrated crude Glur0 off desalt columns
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
Two samples of GluR0 after cobalt column and desalting, concentrated to ~1 mL each.
This Post is Linked By: Gel filtration runs of crude GluR0;GluR0 First Purification SDS-Page Gel;Glur0 purification; ]]>2010-10-21T09:43:25+01:002010-10-21T09:43:25+01:00500ebcf71a288022ab00f2c854c7827ecSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7a4http://biolab.isis.rl.ac.uk/camerons_labblog/13723/Concentrated_crude_Glur0_off_desalt_columns.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13723/Concentrated_crude_Glur0_off_desalt_columns.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13723/Concentrated_crude_Glur0_off_desalt_columns.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13723/Concentrated_crude_Glur0_off_desalt_columns.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13723/Concentrated_crude_Glur0_off_desalt_columns.xml?revision=137231372513725Combined Glur0 fractions from Gel filtration1
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
t
This Post is Linked By: Gel filtration runs of crude GluR0;GluR0 First Purification SDS-Page Gel;Concentration and dialysis of GluR0 samples; ]]>2010-10-21T09:47:51+01:002010-10-21T09:47:51+01:005a89635c5b3c031af1246a8237b0a32e1SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7a5http://biolab.isis.rl.ac.uk/camerons_labblog/13725/Combined_Glur0_fractions_from_Gel_filtration1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13725/Combined_Glur0_fractions_from_Gel_filtration1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13725/Combined_Glur0_fractions_from_Gel_filtration1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13725/Combined_Glur0_fractions_from_Gel_filtration1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13725/Combined_Glur0_fractions_from_Gel_filtration1.xml?revision=137251372613726Combined Glur0 fractions from Gel filtration2
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
t
This Post is Linked By: Gel filtration runs of crude GluR0;GluR0 First Purification SDS-Page Gel;Concentration and dialysis of GluR0 samples; ]]>2010-10-21T09:48:19+01:002010-10-21T09:48:19+01:005ba2b86be4aac18f9683936e2186857b0SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7a6http://biolab.isis.rl.ac.uk/camerons_labblog/13726/Combined_Glur0_fractions_from_Gel_filtration2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13726/Combined_Glur0_fractions_from_Gel_filtration2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13726/Combined_Glur0_fractions_from_Gel_filtration2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13726/Combined_Glur0_fractions_from_Gel_filtration2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13726/Combined_Glur0_fractions_from_Gel_filtration2.xml?revision=137261372813728Wide range Sigma Protein markers
Materials
cameronneylon.netCameron NeylonMaterial: Solution
S8445-1VL
Lot: 058K6011
Sigma Marker Wide Range 6.5k-200k
This Post is Linked By: GluR0 First Purification SDS-Page Gel; ]]>2010-10-21T10:06:13+01:002010-10-21T10:06:13+01:005e1197b0b22786cd36a83a32738961281Solutionhttp://biolab.isis.rl.ac.uk/uri/7a8http://biolab.isis.rl.ac.uk/camerons_labblog/13728/Wide_range_Sigma_Protein_markers.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13728/Wide_range_Sigma_Protein_markers.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13728/Wide_range_Sigma_Protein_markers.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13728/Wide_range_Sigma_Protein_markers.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13728/Wide_range_Sigma_Protein_markers.xml?revision=137281372713735Gel filtration runs of crude GluR0
Procedure
cameronneylon.netCameron NeylonProcedure: Protein_Purification
Project: Glur0
The two samples Concentrated crude Glur0 off desalt columns were run separate on the S200 column. In both cases it appears there is a large quantity of aggregate and only a small amount of the correct peak at 11.8 mL. There appears to be some material but not a whole lot. Fractions from the two peaks (A11, A12, B12, B11 from GF1 and D8-D3 from GF2) were combined to give Combined Glur0 fractions from Gel filtration1 and Combined Glur0 fractions from Gel filtration2.

First Gel Filtration Run (1 mL fractions):
GelFilter1 Image


Second Gel Filtration Run (0.5 mL fractions):
GelFilter2 Image


The peak at 11.8 mL is assigned as GluR0. The earlier peak is aggregate and the later peaks are either monomer or DM micelles (as seen in conductivity)
This Post is Linked By: Second Purification of GluR0 - 2 November;Third purification of GluR0 - 3 November; ]]>2010-10-21T09:48:50+01:002010-10-21T12:38:04+01:005d86a4b4156cd914c1060cbed67f97577Protein_PurificationGlur0http://biolab.isis.rl.ac.uk/data/2321.xmlhttp://biolab.isis.rl.ac.uk/data/2323.xmlhttp://biolab.isis.rl.ac.uk/data/2325.xmlhttp://biolab.isis.rl.ac.uk/data/2327.xmlhttp://biolab.isis.rl.ac.uk/uri/7a7http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=13727http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=13732http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=13733http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=13731http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=13734http://biolab.isis.rl.ac.uk/camerons_labblog/13727/Gel_filtration_runs_of_crude_GluR0.xml?revision=137351372913738GluR0 First Purification SDS-Page Gel
Procedures
cameronneylon.netCameron NeylonProcedure: SDS_PAGE
Dna: Glur0


Lane		Sampleul

1

2		Wide range Sigma Protein markers20

3		Cleared GluR0 lysate20

4		Fractions C1/C2 from Glur0 Cobalt column20 C1

5		Fractions C1/C2 from Glur0 Cobalt column20 C2

6

7		Concentrated crude Glur0 off desalt columns20 load for GF1

8		Concentrated crude Glur0 off desalt columns20 load for GF2

9		Combined Glur0 fractions from Gel filtration120

10		Combined Glur0 fractions from Gel filtration220


A 10% SDS-PAGE gel was polymerized according to Sambrook. Samples were loaded in gel loading buffer and the gel run at 180 V for approximately 1 hour.


First photo of gel

]]>2010-10-21T10:08:33+01:002010-10-21T13:54:51+01:005feb96bb0d8e4c5f71b007b47f7f26ae9SDS_PAGEGlur0http://biolab.isis.rl.ac.uk/data/2329.xmlhttp://biolab.isis.rl.ac.uk/uri/7a9http://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.xml?revision=13730http://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.xml?revision=13729http://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.xml?revision=13738http://biolab.isis.rl.ac.uk/camerons_labblog/13729/GluR0_First_Purification_SDSPage_Gel.xml?revision=137371373913739H20 dialysed GluR0 sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
t
This Post is Linked By: Concentration and dialysis of GluR0 samples;Further processing of purified GluR0; ]]>2010-10-21T14:31:20+01:002010-10-21T14:31:20+01:005490dc6a95e9e92259319bf9b99253e23Solutionhttp://biolab.isis.rl.ac.uk/uri/7abhttp://biolab.isis.rl.ac.uk/camerons_labblog/13739/H20_dialysed_GluR0_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13739/H20_dialysed_GluR0_sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13739/H20_dialysed_GluR0_sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13739/H20_dialysed_GluR0_sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13739/H20_dialysed_GluR0_sample.xml?revision=137391374013740D2O Dialysed GluR0 Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
t
This Post is Linked By: Concentration and dialysis of GluR0 samples;Further processing of purified GluR0; ]]>2010-10-21T14:32:26+01:002010-10-21T14:32:26+01:0051c79d85244f7e2d5f7598be54c5c1c07Solutionhttp://biolab.isis.rl.ac.uk/uri/7achttp://biolab.isis.rl.ac.uk/camerons_labblog/13740/D2O_Dialysed_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13740/D2O_Dialysed_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13740/D2O_Dialysed_GluR0_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13740/D2O_Dialysed_GluR0_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13740/D2O_Dialysed_GluR0_Sample.xml?revision=137401373613741Concentration and dialysis of GluR0 samples
Procedure
cameronneylon.netCameron NeylonProcedure: Sample_Preparation
Project: Glur0
The purified fractions Combined Glur0 fractions from Gel filtration1 and Combined Glur0 fractions from Gel filtration2 were combined and concentrated to a total of ~2 mL. The samples were then split into two and dialysed against Tris-HCl pH7.6 100 mM KCl, 5 mM decyl maltoside buffer in H2O or D2O respectively to give H20 dialysed GluR0 sample and D2O Dialysed GluR0 Sample.
]]>2010-10-21T13:52:05+01:002010-10-21T14:32:49+01:0057dc25cb2003211c8dd6becb4008bd4afSample_PreparationGlur0http://biolab.isis.rl.ac.uk/uri/7aahttp://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.xml?revision=13736http://biolab.isis.rl.ac.uk/camerons_labblog/13736/Concentration_and_dialysis_of_GluR0_samples.xml?revision=137411375413754Luke's dialysed H2O GluR0 Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A sample of GluR0 re-purified by Luke from previous samples by gel filtration and dialysed into H2O Buffer.
This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-10-25T12:45:08+01:002010-10-25T12:45:08+01:005074a102d32833127b02115faefbfb551SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7b1http://biolab.isis.rl.ac.uk/camerons_labblog/13754/Lukes_dialysed_H2O_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13754/Lukes_dialysed_H2O_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13754/Lukes_dialysed_H2O_GluR0_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13754/Lukes_dialysed_H2O_GluR0_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13754/Lukes_dialysed_H2O_GluR0_Sample.xml?revision=137541375513755Luke's dialysed D2O GluR0 Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Dna: Glur0
A sample of GluR0 re-purified by Luke from previous samples by gel filtration and dialysed into D2O Buffer.
This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-10-25T12:45:37+01:002010-10-25T12:45:37+01:005b07409ec12e28625657964061bf8a916SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7b2http://biolab.isis.rl.ac.uk/camerons_labblog/13755/Lukes_dialysed_D2O_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13755/Lukes_dialysed_D2O_GluR0_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13755/Lukes_dialysed_D2O_GluR0_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13755/Lukes_dialysed_D2O_GluR0_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13755/Lukes_dialysed_D2O_GluR0_Sample.xml?revision=137551375613756Luke's dialysed H2O GluR0 Sample with 1 mM Glutamate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A sample of GluR0 with 1 mM glutamate re-purified by Luke from previous samples by gel filtration and dialysed into H2O Buffer with 1 mM glutamate.
]]>2010-10-25T12:46:18+01:002010-10-25T12:46:18+01:005c4643db8522d385f6d99453182a15a4cSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7b3http://biolab.isis.rl.ac.uk/camerons_labblog/13756/Lukes_dialysed_H2O_GluR0_Sample_with_1_mM_Glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13756/Lukes_dialysed_H2O_GluR0_Sample_with_1_mM_Glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13756/Lukes_dialysed_H2O_GluR0_Sample_with_1_mM_Glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13756/Lukes_dialysed_H2O_GluR0_Sample_with_1_mM_Glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13756/Lukes_dialysed_H2O_GluR0_Sample_with_1_mM_Glutamate.xml?revision=137561375713757Luke's dialysed D2O GluR0 Sample with 1 mM Glutamate
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A sample of GluR0 with 1 mM glutamate re-purified by Luke from previous samples by gel filtration and dialysed into D2O Buffer with 1 mM Glutamate.
]]>2010-10-25T12:47:01+01:002010-10-25T12:47:01+01:00523f2916e0a48b289e65ae780cbe1de18SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7b4http://biolab.isis.rl.ac.uk/camerons_labblog/13757/Lukes_dialysed_D2O_GluR0_Sample_with_1_mM_Glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13757/Lukes_dialysed_D2O_GluR0_Sample_with_1_mM_Glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13757/Lukes_dialysed_D2O_GluR0_Sample_with_1_mM_Glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13757/Lukes_dialysed_D2O_GluR0_Sample_with_1_mM_Glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13757/Lukes_dialysed_D2O_GluR0_Sample_with_1_mM_Glutamate.xml?revision=137571370213758Glur0 purification
Procedure
cameronneylon.netCameron NeylonProcedure: Protein_Purification
Project: Glur0
The pellets from yesterdays growth (2 x 2L, around 5 g) were resuspended in 40 mL of extraction buffer (50 mM Tris-HCl pH 7.6, 100 mM KCl, 40 mM decyl maltoside) and allowed to thaw. The pellet was resuspended over about 1 hour. The suspension was lysed by sonication (20 minutes total, 10s on, 10s off, 7 C) and then spun (19krpm, 21 minutes, 4 C) to give Cleared GluR0 lysate.

The cleared lysate was loaded in three batches onto 2 x 5 mL columns equilibrated in buffer A (20 mM Tris-HCl pH 7.6, 100 mM KCl) with 30 mM imidazole and washed with around 8 column volumes of buffer A. The protein was eluted manually by changing to buffer A with 300 mM imidazole to yield Fractions C1/C2 from Glur0 Cobalt column.

Glur0 Cobalt Trace


After the absorbance settled the column was washed with buffer A (20 mL), buffer B (20 mL) and buffer A (8 mL) before being transferred into 20% EtOH.

Desalt columns (2 x 5 mL) were equilibrated in Buffer A and the combined fractions loaded in three lots of 3.5 mL. UV was monitored at 260, 280, and 320 and the fractions manually advanced to ensure the minimal volume collected. The A260 seems somewhat high relative to the A280 which is somewhat concerning. The gel filtration column should give us the right answer tho.

Glur0 Desalt Traces


The selected fractions (~7 mL total) were concentrated using spin concentrators to two samples with (Concentrated crude Glur0 off desalt columns) with a final volume of ~1 mL each.
This Post is Linked By: Fractions C1/C2 from Glur0 Cobalt column;Second Purification of GluR0 - 2 November;Third purification of GluR0 - 3 November; ]]>2010-10-20T13:15:18+01:002010-10-25T13:33:11+01:0059a3d516e0d11dda5adea6bc7493db881Protein_PurificationGlur0http://biolab.isis.rl.ac.uk/data/2312.xmlhttp://biolab.isis.rl.ac.uk/data/2314.xmlhttp://biolab.isis.rl.ac.uk/data/2316.xmlhttp://biolab.isis.rl.ac.uk/data/2318.xmlhttp://biolab.isis.rl.ac.uk/uri/7a1http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13702http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13704http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13705http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13707http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13708http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13709http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13710http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13711http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13713http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13714http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13716http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13715http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13717http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13718http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=13724http://biolab.isis.rl.ac.uk/camerons_labblog/13702/Glur0_purification.xml?revision=137581381613816GluR0-H2O sample after dialysis
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A H2O GLur0 sample after dialysis
This Post is Linked By: Further processing of purified GluR0;UV-Vis measurement of GluR0 Samples; ]]>2010-11-01T14:10:27+00:002010-11-01T14:10:27+00:005b889f99120268c38586203a9b2e8e9feSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7bchttp://biolab.isis.rl.ac.uk/camerons_labblog/13816/GluR0H2O_sample_after_dialysis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13816/GluR0H2O_sample_after_dialysis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13816/GluR0H2O_sample_after_dialysis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13816/GluR0H2O_sample_after_dialysis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13816/GluR0H2O_sample_after_dialysis.xml?revision=138161381713819GluR0-D2O sample after dialysis
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
A GluR0 sample in D2O buffer after dialysis
This Post is Linked By: Further processing of purified GluR0;UV-Vis measurement of GluR0 Samples; ]]>2010-11-01T14:11:15+00:002010-11-01T14:12:36+00:00516e66058feb47665b54f13c3501cd731SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7bdhttp://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.xml?revision=13817http://biolab.isis.rl.ac.uk/camerons_labblog/13817/GluR0D2O_sample_after_dialysis.xml?revision=138191381813818Further processing of purified GluR0
Procedures
cameronneylon.netCameron NeylonProcedure: Sample_Preparation
Project: Glur0
The samples H20 dialysed GluR0 sample and D2O Dialysed GluR0 Sample were taken off dialysis. Both had significant amounts of precipitate and were spun for 20' at 14krpm in a bench top centrifuge to clear. The resultant supernatant was kept to give GluR0-H2O sample after dialysis and GluR0-DA2O sample after dialysis.
]]>2010-11-01T14:12:22+00:002010-11-01T14:12:22+00:00516170dbeedb95b9fc9e12aaaccfc988eSample_PreparationGlur0http://biolab.isis.rl.ac.uk/uri/7behttp://biolab.isis.rl.ac.uk/camerons_labblog/13818/Further_processing_of_purified_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13818/Further_processing_of_purified_GluR0.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13818/Further_processing_of_purified_GluR0.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13818/Further_processing_of_purified_GluR0.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13818/Further_processing_of_purified_GluR0.xml?revision=138181384613846Final GluR0 dialysis buffer - H2O
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
The final buffer into which GluR0 was dialysed for the H2O sample. 20 mM Tris-HCl pH 7.4 100 mM KCl 5 mM decyl maltoside.
This Post is Linked By: Second Purification of GluR0 - 2 November; ]]>2010-11-08T11:09:46+00:002010-11-08T11:09:46+00:0052668088a8e38c9589562af745388227fSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7cahttp://biolab.isis.rl.ac.uk/camerons_labblog/13846/Final_GluR0_dialysis_buffer__H2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13846/Final_GluR0_dialysis_buffer__H2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13846/Final_GluR0_dialysis_buffer__H2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13846/Final_GluR0_dialysis_buffer__H2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13846/Final_GluR0_dialysis_buffer__H2O.xml?revision=138461384713847Final GluR0 dialysis buffer - D2O
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
The final buffer into which GluR0 was dialysed for the D2O sample. 20 mM Tris-HCl pD 7.4 100 mM KCl 5 mM decyl maltoside.
This Post is Linked By: Second Purification of GluR0 - 2 November; ]]>2010-11-08T11:10:26+00:002010-11-08T11:10:26+00:00579d9ac23469d0e3668c50c34c0cd792dSolutionGlur0http://biolab.isis.rl.ac.uk/uri/7cbhttp://biolab.isis.rl.ac.uk/camerons_labblog/13847/Final_GluR0_dialysis_buffer__D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13847/Final_GluR0_dialysis_buffer__D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13847/Final_GluR0_dialysis_buffer__D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13847/Final_GluR0_dialysis_buffer__D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13847/Final_GluR0_dialysis_buffer__D2O.xml?revision=138471382213848Second Purification of GluR0 - 2 November
Procedure
cameronneylon.netCameron NeylonProcedure: Protein_Purification
Project: Glur0
Two pellets (~15g) of cells from the second growth of GluR0 were resuspended, sonicated, lysed and purified as described in Glur0 purification and Gel filtration runs of crude GluR0. The resulting gel filtered fractions were combined and concentrated and then dialysed against 250 mL of H2O or D2O buffer for two days, then against a second 250 mL of Final GluR0 dialysis buffer - H2O and Final GluR0 dialysis buffer - D2O to give 2nd Glur0 purification - H2O Sample and 2nd Glur0 purification - D2O Sample respectively.

Cobalt run:
Glur0 Cobalt Traces


Desalt:
Glur0 Desalt Traces


Gel filtration #1:
GelFilter1 Image


Gel filtration #2:
GelFilter2 Image

This Post is Linked By: 2nd Glur0 purification - H2O Sample;2nd Glur0 purification - D2O Sample; ]]>2010-11-03T11:10:09+00:002010-11-08T11:11:17+00:005f7468cd482d0de899c00cf62eea90072Protein_PurificationGlur0http://biolab.isis.rl.ac.uk/data/2344.xmlhttp://biolab.isis.rl.ac.uk/data/2346.xmlhttp://biolab.isis.rl.ac.uk/data/2348.xmlhttp://biolab.isis.rl.ac.uk/data/2350.xmlhttp://biolab.isis.rl.ac.uk/data/2352.xmlhttp://biolab.isis.rl.ac.uk/data/2354.xmlhttp://biolab.isis.rl.ac.uk/data/2356.xmlhttp://biolab.isis.rl.ac.uk/data/2358.xmlhttp://biolab.isis.rl.ac.uk/uri/7c1http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13823http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13824http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13825http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13826http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13827http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13831http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13828http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13829http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13830http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=13822http://biolab.isis.rl.ac.uk/camerons_labblog/13822/Second_Purification_of_GluR0__2_November.xml?revision=1384813844138442nd Glur0 purification - H2O Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
Glur0 sample after dialysis from Second Purification of GluR0 - 2 November
This Post is Linked By: Second Purification of GluR0 - 2 November;UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T11:06:51+00:002010-11-08T11:06:51+00:0051b4d67db58979e2b4d788c533d049844SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7c8http://biolab.isis.rl.ac.uk/camerons_labblog/13844/2nd_Glur0_purification__H2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13844/2nd_Glur0_purification__H2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13844/2nd_Glur0_purification__H2O_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13844/2nd_Glur0_purification__H2O_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13844/2nd_Glur0_purification__H2O_Sample.xml?revision=1384413845138452nd Glur0 purification - D2O Sample
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: Glur0
Sample of GLur0 in D2O buffer after dialysis from Second Purification of GluR0 - 2 November
This Post is Linked By: Second Purification of GluR0 - 2 November;UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T11:07:42+00:002010-11-08T11:07:42+00:005803670c01f287dc309f9331e92c48c71SolutionGlur0http://biolab.isis.rl.ac.uk/uri/7c9http://biolab.isis.rl.ac.uk/camerons_labblog/13845/2nd_Glur0_purification__D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13845/2nd_Glur0_purification__D2O_Sample.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13845/2nd_Glur0_purification__D2O_Sample.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13845/2nd_Glur0_purification__D2O_Sample.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13845/2nd_Glur0_purification__D2O_Sample.xml?revision=138451383213843Third purification of GluR0 - 3 November
Procedure
cameronneylon.netCameron NeylonProcedure: Protein_Purification
Project: Glur0
Two more cell pellets from the second growth were lysed and the lysate cleared as decribed. The protein was purified as described in Glur0 purification and Gel filtration runs of crude GluR0 except that the loading onto the cobalt column involved some jiggery pokery with buffers.

The cleared lysate was loaded in 30 mM imidazole buffer but as this was running out the column was then washed with buffer without imidazole. A small amount of buffer with imidazole was then added to bring the concentration of the buffer to ~30 mM imidazole before eluting with 300 mM imidazole buffer.

Due to problems in the Gel filtration run this purification was ultimately abandoned

Cobalt:

Glur0 Cobalt Trace


Desalt:

Glur0 Desalt image

]]>2010-11-03T11:26:25+00:002010-11-08T11:05:57+00:00518c78c000dac045700662b6fdb475424Protein_PurificationGlur0http://biolab.isis.rl.ac.uk/data/2360.xmlhttp://biolab.isis.rl.ac.uk/data/2362.xmlhttp://biolab.isis.rl.ac.uk/data/2364.xmlhttp://biolab.isis.rl.ac.uk/data/2366.xmlhttp://biolab.isis.rl.ac.uk/uri/7c2http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13832http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13834http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13835http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13836http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13837http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13833http://biolab.isis.rl.ac.uk/camerons_labblog/13832/Third_purification_of_GluR0__3_November.xml?revision=13843138501385313754 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
Data from UV-Vis measurement of GluR0 Samples

13754.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:10:33+00:002010-11-08T12:11:13+00:005e826a1dee59202e1ce5e9291209f40d5UV-VISGlur0http://biolab.isis.rl.ac.uk/data/2373.xmlhttp://biolab.isis.rl.ac.uk/data/2375.xmlhttp://biolab.isis.rl.ac.uk/uri/7cdhttp://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.xml?revision=13850http://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.xml?revision=13851http://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.xml?revision=13852http://biolab.isis.rl.ac.uk/camerons_labblog/13850/13754_UVVis.xml?revision=13853138541385713755 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
UV-Vis data from UV-Vis measurement of GluR0 Samples

13755.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:16:00+00:002010-11-08T12:16:26+00:005a4a7610ac98c2d8136bd989bf5803694UV-VISGlur0http://biolab.isis.rl.ac.uk/data/2377.xmlhttp://biolab.isis.rl.ac.uk/data/2379.xmlhttp://biolab.isis.rl.ac.uk/uri/7cehttp://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.xml?revision=13854http://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.xml?revision=13855http://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.xml?revision=13856http://biolab.isis.rl.ac.uk/camerons_labblog/13854/13755_UVVis.xml?revision=13857138581386113816 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
Data from UV-Vis measurement of GluR0 Samples

13816.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:24:48+00:002010-11-08T12:25:13+00:00522418dc86744810a2c74e9e09e906e2eUV-VISGlur0http://biolab.isis.rl.ac.uk/data/2381.xmlhttp://biolab.isis.rl.ac.uk/data/2383.xmlhttp://biolab.isis.rl.ac.uk/uri/7cfhttp://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.xml?revision=13859http://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.xml?revision=13860http://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.xml?revision=13861http://biolab.isis.rl.ac.uk/camerons_labblog/13858/13816_UVVis.xml?revision=13858138621386513817 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
Data from UV-Vis measurement of GluR0 Samples

13817.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:28:19+00:002010-11-08T12:28:45+00:00564a169977cd859d1754b0105b4c86890UV-VISGlur0http://biolab.isis.rl.ac.uk/data/2385.xmlhttp://biolab.isis.rl.ac.uk/data/2387.xmlhttp://biolab.isis.rl.ac.uk/uri/7d0http://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.xml?revision=13862http://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.xml?revision=13863http://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.xml?revision=13864http://biolab.isis.rl.ac.uk/camerons_labblog/13862/13817_UVVis.xml?revision=13865138661386913844 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
Data from UV-Vis measurement of GluR0 Samples

13844.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:31:40+00:002010-11-08T12:32:06+00:00506767c7c2a00fed3f964007143e6939fUV-VISGlur0http://biolab.isis.rl.ac.uk/data/2389.xmlhttp://biolab.isis.rl.ac.uk/data/2391.xmlhttp://biolab.isis.rl.ac.uk/uri/7d1http://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.xml?revision=13866http://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.xml?revision=13867http://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.xml?revision=13868http://biolab.isis.rl.ac.uk/camerons_labblog/13866/13844_UVVis.xml?revision=13869138701387313845 UV-Vis
Data
cameronneylon.netCameron NeylonData Type: UV-VIS
Project: Glur0
Data from UV-Vis measurement of GluR0 Samples

13845.xls

This Post is Linked By: UV-Vis measurement of GluR0 Samples; ]]>2010-11-08T12:35:38+00:002010-11-08T12:36:03+00:005a823e350808b8f814275e126038f9d5bUV-VISGlur0http://biolab.isis.rl.ac.uk/data/2393.xmlhttp://biolab.isis.rl.ac.uk/data/2395.xmlhttp://biolab.isis.rl.ac.uk/uri/7d2http://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.xml?revision=13870http://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.xml?revision=13871http://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.xml?revision=13872http://biolab.isis.rl.ac.uk/camerons_labblog/13870/13845_UVVis.xml?revision=138731384913874UV-Vis measurement of GluR0 Samples
Procedure
cameronneylon.netCameron NeylonProcedure: UV-Vis
Project: Glur0
Glur0 samples from the previous preparations were determined by UV-Vis on teh GeneQuant 1300 in a 0.5 cm microcell.


SampleData
Luke's dialysed H2O GluR0 Sample13754 UV-Vis
Luke's dialysed D2O GluR0 Sample13755 UV-Vis
GluR0-H2O sample after dialysis13816 UV-Vis
GluR0-D2O sample after dialysis13817 UV-Vis
2nd Glur0 purification - H2O Sample13844 UV-Vis
2nd Glur0 purification - D2O Sample13845 UV-Vis

This Post is Linked By: 13754 UV-Vis;13755 UV-Vis;13816 UV-Vis;13817 UV-Vis;13844 UV-Vis;13845 UV-Vis; ]]>2010-11-08T12:06:58+00:002010-11-08T12:36:19+00:0057696b72916efe45f62115e5486df0036UV-VisGlur0http://biolab.isis.rl.ac.uk/uri/7cchttp://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.xml?revision=13849http://biolab.isis.rl.ac.uk/camerons_labblog/13849/UVVis_measurement_of_GluR0_Samples.xml?revision=138741392213925UV-Vis of Tus SANS samples from Quokka
Data
cameronneylon.netCameron NeylonData Type: UV-Vis
The SANS samples and background were run in 1 mm banjo cells on the Cary UV-Vis
]]>2010-11-18T22:47:10+00:002010-11-18T22:48:03+00:005c8a2238632a011c3e9ad6e9fa4f0f50aUV-Vishttp://biolab.isis.rl.ac.uk/data/2407.xmlhttp://biolab.isis.rl.ac.uk/data/2409.xmlhttp://biolab.isis.rl.ac.uk/uri/7ddhttp://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.xml?revision=13922http://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.xml?revision=13923http://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.xml?revision=13924http://biolab.isis.rl.ac.uk/camerons_labblog/13922/UVVis_of_Tus_SANS_samples_from_Quokka.xml?revision=139251401614016Glur0 sample as purified
Materials
cameronneylon.netCameron NeylonMaterial: Solution
The Glur0 prepared and concentrated to about 1 mg/mL
This Post is Linked By: Synchrotron CD titration of GLur0 with glutamate;CD Beamtime - BM23 16 Feb 2011; ]]>2011-02-16T13:21:02+00:002011-02-16T13:21:02+00:005ba7b9a62003ac4e2f71e111f8d603375Solutionhttp://biolab.isis.rl.ac.uk/uri/838http://biolab.isis.rl.ac.uk/camerons_labblog/14016/Glur0_sample_as_purified.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14016/Glur0_sample_as_purified.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14016/Glur0_sample_as_purified.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14016/Glur0_sample_as_purified.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14016/Glur0_sample_as_purified.xml?revision=140161402014021Synchrotron CD titration of GLur0 with glutamate
Procedure
cameronneylon.netCameron NeylonProcedure: Synchrotron_CD
Project: Glur0
A titration of glutamate with glur0 was run in a 0.1 mm cylindrical cell with 0.5 mm slits with wavelength range from 185-265 nm. Each sample was made with 90 uL of Glur0 sample as purified which was diluted with 10 uL of buffer plus glutamate to give the final appropriate concentration. In each case at least six scans were run with 1 sec integration time and 1 nm wavelength steps.


SampleRunEquivalents (approx)[Glutamate](uM)
1000
11110
1210100]
14330
170.33
18660
1930300
2050500


Control runs:


SampleRun
100 uM Glutamate13
Buffer15
Buffer with better cleaned out cell16
1 mM glutamate21

This Post is Linked By: CD Beamtime - BM23 16 Feb 2011; ]]>2011-02-17T10:58:36+00:002011-02-17T10:59:10+00:005c1fb886c0397d19bd524904a1628c3d6Synchrotron_CDGlur0http://biolab.isis.rl.ac.uk/uri/83bhttp://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.xml?revision=14021http://biolab.isis.rl.ac.uk/camerons_labblog/14020/Synchrotron_CD_titration_of_GLur0_with_glutamate.xml?revision=140201402214022In situ glutamate titration with 1 mm cell
Procedure
cameronneylon.netCameron NeylonProcedure: Synchrotron_CD
Project: Glur0
A titration was run from 0 to 3 equivalents of glutamate (nominal) in a 1mm pathlength cell from 200-265 nm


This Post is Linked By: CD Beamtime - BM23 16 Feb 2011; ]]>2011-02-17T11:51:22+00:002011-02-17T11:51:22+00:0053647cd75e0266bc60304d297344444d6Synchrotron_CDGlur0http://biolab.isis.rl.ac.uk/uri/83chttp://biolab.isis.rl.ac.uk/camerons_labblog/14022/In_situ_glutamate_titration_with_1_mm_cell.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14022/In_situ_glutamate_titration_with_1_mm_cell.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14022/In_situ_glutamate_titration_with_1_mm_cell.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14022/In_situ_glutamate_titration_with_1_mm_cell.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14022/In_situ_glutamate_titration_with_1_mm_cell.xml?revision=140221401714026CD Beamtime - BM23 16 Feb 2011
Procedure
cameronneylon.netCameron NeylonProcedure: Synchrotron_CD
Project: Glur0
160211b04-05
Started by running buffer and then some samples of Glur0 sample as purified. Reasonable spectra with 10 collections but not down to beyond 186 nm due to presence of chloride.

160211b06-08
Ran 20 scans. Saw some evidence of slight degradation. Not a problem for titration as we need to replace sample for each glutamate concentrartion but could be an issue for temperature scans. Will therefore need to reduce beam intensity if we want to do that.

160211b10
Removed Lens, still on 0.5 mm slit width, repeated Glur0 experiment 20 scans to show no degradation (note first 2 scan showed movement..). This scan is also the first titration point - no gluatamate.

Ex situ titration
Then ran Synchrotron CD titration of GLur0 with glutamate with GluR0 and glutamate, separate samples as noted. After subtraction we see changes around 210 nm, possibly two binding transitions followed by unfolding.

Thermal stability scan of GluR0 alone
With 90:10 (Glur0:Buffer) sample ran a thermal scan in 5 degree steps with 4 second scans up and down. We see one clear unfolding transition (not reversible) with possibility of other transitions. Will compare to sample with glutamate.

In situ titration
Following above titration decided to do in situ titration with longer path length from 200-265 nm. 1 mm cell seems fine so then run In situ glutamate titration with 1 mm cell.

Results here were not consistent with yesterday but went onto a higher concentration run which was abandoned when we realised that kinetics of the reaction were clearly slow (run left over dinner had much stronger signal). Therefore going to set up samples to sit overnight and run in the morning.
]]>2011-02-16T17:12:37+00:002011-02-17T21:04:55+00:0057ecf9b54298fa6a4675b44f34e35ad77Synchrotron_CDGlur0http://biolab.isis.rl.ac.uk/uri/839http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=14017http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=14019http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=14024http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=14023http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=14025http://biolab.isis.rl.ac.uk/camerons_labblog/14017/CD_Beamtime__BM23_16_Feb_2011.xml?revision=140261411314114GF Buffer for purification of SMALP samples
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: smalp
2L of Sodium Phosphate buffer pH 8.0, 0.2 M NaCl prepared with:

0.94 g monobasic NaPhos monohydrate
13.2 g dibasic NaPhos (zero hydrate)
from http://home.fuse.net/clymer/buffers/phos2.html

and 23.38g NaCl
This Post is Linked By: Purification of SMALP samples A4 and A8 for I22;Purification of SMALP sample B3; ]]>2011-05-14T10:14:47+01:002011-05-14T10:15:57+01:0058cfd551b1fd9086b43f27ca45aedb4f8Solutionsmalphttp://biolab.isis.rl.ac.uk/uri/893http://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.xml?revision=14113http://biolab.isis.rl.ac.uk/camerons_labblog/14113/GF_Buffer_for_purification_of_SMALP_samples.xml?revision=141141411514127Purification of SMALP samples A4 and A8 for I22
Procedure
cameronneylon.netCameron NeylonProcedure: Purification
Project: smalp
The samples A4 (DMPC:60% Mono-olein:40% lipid content) and A8 (DMPC:20% MO:80%) from Ilaria were spun at 20k x g for 15 minutes to remove particulate that appeared to have formed. The S200 column was prepared with GF Buffer for purification of SMALP samples and the pressure seems OK when run at 0.4 mL/min. The 1 mL sample loading loop was put on the instrument.

Loaded ~150 uL of A4 (file mislabelled as A8) for test run on column. Very small quantity of presumed SMALP eluting at 18.5 mL. Allowed to run out, saw presumed polymer peak at 26 mL.

Followed up with a ~600 uL injection of A4 in the same file record. Many more peaks in this case but still a main peak, reasonable amounts at around 18 mL. Second peak at ~14 mL another species of SMALP perhaps?

One more ~500 uL injection of A4, this time large aggregate peak in void volume. Not so good, but reasonable yield of desired species.

Re-span A8 to remove aggregate which seems to re-form based on previous runs (or just re-suspends itself). Loading ~600 uL onto column and collecting 1 mL fractions from 125 mL.

smalp-gel-filtration-firstruns-140511.jpg
first-runs-11-05-14.asc


Ran sample A8 (after a further spin) using new method Sup200CamSmalp with a ~700 uL injection of sample.

smalp-gel-filtration-lasta8run-140511.jpg
lasta8-run-11-05-14.asc

]]>2011-05-14T14:40:31+01:002011-05-15T10:59:42+01:00529ebdf1b80649d3dfcaea6f02a3eb458Purificationsmalphttp://biolab.isis.rl.ac.uk/data/2589.xmlhttp://biolab.isis.rl.ac.uk/data/2591.xmlhttp://biolab.isis.rl.ac.uk/data/2593.xmlhttp://biolab.isis.rl.ac.uk/data/2595.xmlhttp://biolab.isis.rl.ac.uk/data/2597.xmlhttp://biolab.isis.rl.ac.uk/uri/894http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14115http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14116http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14117http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14118http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14120http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14123http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14122http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14124http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14125http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=14126http://biolab.isis.rl.ac.uk/camerons_labblog/14115/Purification_of_SMALP_samples_A4_and_A8_for_I22.xml?revision=141271412114137Purification of SMALP sample B3
Procedure
cameronneylon.netCameron NeylonProcedure: Purification
Project: smalp
Using the new program Sup200CamSmalp a ~600 uL sample of SMALP-B3 (70% DMPC 30% DMPG) was run with GF Buffer for purification of SMALP samples as eluent.

smalp-gel-filtration-1stb3run-140511.jpg
1stb3-run-11-05-14.asc


This looked to have not great resolution of the void volume peak from the apparent SMALP eluting at ~10 mL. Note that this suggests that these smalps are significantly larger than the previous ones.

Adjusted the fractions to start collecting earlier and re-ran with a sample of ~300 uL for second run.

smalp-gel-filtration-2ndb3run-140511.jpg
2ndb3-run-11-05-14.asc


As resolution was not a whole lot better did a final run of around ~500 uL

smalp-gel-filtration-3rdb3run-140511.jpg
3rdb3-run-11-05-14.asc

]]>2011-05-15T10:44:59+01:002011-05-15T14:57:41+01:0056a9493c1b217c52e27a85d1baf9adc5fPurificationsmalphttp://biolab.isis.rl.ac.uk/data/2599.xmlhttp://biolab.isis.rl.ac.uk/data/2601.xmlhttp://biolab.isis.rl.ac.uk/data/2603.xmlhttp://biolab.isis.rl.ac.uk/data/2605.xmlhttp://biolab.isis.rl.ac.uk/data/2607.xmlhttp://biolab.isis.rl.ac.uk/data/2609.xmlhttp://biolab.isis.rl.ac.uk/uri/896http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14121http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14129http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14130http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14128http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14131http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14132http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14134http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14133http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14135http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=14136http://biolab.isis.rl.ac.uk/camerons_labblog/14121/Purification_of_SMALP_sample_B3.xml?revision=141371413814138Purification of SMALP samples A7 for I22
Procedure
cameronneylon.netCameron NeylonProcedure: Purification
Project: smalp
Purification proceeded as above for A7, initial load of ~300 uL
]]>2011-05-15T19:01:39+01:002011-05-15T19:01:39+01:00597c45397204d54a73f4e5c69d19d87f9Purificationsmalphttp://biolab.isis.rl.ac.uk/uri/897http://biolab.isis.rl.ac.uk/camerons_labblog/14138/Purification_of_SMALP_samples_A7_for_I22.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14138/Purification_of_SMALP_samples_A7_for_I22.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14138/Purification_of_SMALP_samples_A7_for_I22.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14138/Purification_of_SMALP_samples_A7_for_I22.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14138/Purification_of_SMALP_samples_A7_for_I22.xml?revision=14138281814143SAXS on I22 template
Templates
cameron.neylon.myopenid.comCameron NeylonProcedures]] [[Procedure>SAXS]] [[Instrument>I22]] [[Project>smalp]]]]>

SampleFramesExposure time I22 Raw Run #Data
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[[box=5]][[box=3]][[Box=3]][[Box=5]][[Blog]]
[[box=5]][[box=3]][[Box=3]][[Box=5]][[Blog]]
[[box=5]][[box=3]][[Box=3]][[Box=5]][[Blog]]
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[[Section>Procedures]]
[[Procedure>SAXS]]
[[Instrument>I22]]
[[Project>smalp]]
]]>2009-02-02T14:19:36+00:002011-05-16T14:41:42+01:005713904b4eaea1ddc7d7b357c80d97085http://biolab.isis.rl.ac.uk/uri/15chttp://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=2818http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=2898http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11531http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11532http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11533http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11534http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11535http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11537http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=11538http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=14140http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=14141http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=14142http://biolab.isis.rl.ac.uk/camerons_labblog/2818/SAXS_on_I22_template.xml?revision=14143282014144I22 Data template
Templates
cameron.neylon.myopenid.comCameron NeylonData]] [[Data Type>SAXS_Diamond]] [[Instrument>I22]] [[Project>smalp]]]]>
Radially averaged but not background subtracted
Raw data fileData file[[box]][[box]]

[[Section>Data]]
[[Data Type>SAXS_Diamond]]
[[Instrument>I22]]
[[Project>smalp]]
]]>2009-02-02T14:28:28+00:002011-05-16T14:58:14+01:0055d2d6c06559989818f670aa3297003abhttp://biolab.isis.rl.ac.uk/uri/15dhttp://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2820http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2821http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2829http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2894http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2929http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=2953http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=11579http://biolab.isis.rl.ac.uk/camerons_labblog/2820/I22_Data_template.xml?revision=141441414514147I22-29552
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29552
J52001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T14:59:13+01:002011-05-16T15:00:39+01:00536614b74f598bc927cf429fbfb2894f2SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2612.xmlhttp://biolab.isis.rl.ac.uk/uri/899http://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.xml?revision=14145http://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.xml?revision=14146http://biolab.isis.rl.ac.uk/camerons_labblog/14145/I2229552.xml?revision=141471414814150I22 29553 - b4
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29553
J53001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:03:18+01:002011-05-16T15:03:35+01:00501d80fe4fe3890f364a6866e36336f10SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2614.xmlhttp://biolab.isis.rl.ac.uk/uri/89ahttp://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.xml?revision=14148http://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.xml?revision=14149http://biolab.isis.rl.ac.uk/camerons_labblog/14148/I22_29553__b4.xml?revision=141501415114153I22 29554 - buffer
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29554
J54001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:04:00+01:002011-05-16T15:04:18+01:00544e76401eb82d6960a824e0db3ecf647SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2616.xmlhttp://biolab.isis.rl.ac.uk/uri/89bhttp://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.xml?revision=14151http://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.xml?revision=14152http://biolab.isis.rl.ac.uk/camerons_labblog/14151/I22_29554__buffer.xml?revision=141531423914241I22 29590 - A4
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29590
J89001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:34:55+01:002011-05-16T17:35:11+01:0051bc453259bced3b7e257defa78666a12SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2676.xmlhttp://biolab.isis.rl.ac.uk/uri/8b9http://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.xml?revision=14240http://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.xml?revision=14241http://biolab.isis.rl.ac.uk/camerons_labblog/14239/I22_29590__A4.xml?revision=142391415414156I22 29555 - b3
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29555
J55001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:04:57+01:002011-05-16T15:05:21+01:005308c26bdfdb8cb0a88e388d0ed5e6b10SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2618.xmlhttp://biolab.isis.rl.ac.uk/uri/89chttp://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.xml?revision=14154http://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.xml?revision=14155http://biolab.isis.rl.ac.uk/camerons_labblog/14154/I22_29555__b3.xml?revision=141561415714159I22 29556 - empty capillary
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29556
J56001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:06:20+01:002011-05-16T15:07:38+01:0055ebd200b5dfbbcdffb131ad9d4a13d17SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2620.xmlhttp://biolab.isis.rl.ac.uk/uri/89dhttp://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.xml?revision=14157http://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.xml?revision=14158http://biolab.isis.rl.ac.uk/camerons_labblog/14157/I22_29556__empty_capillary.xml?revision=141591416014162I22 29557 - b2
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29557`
J57001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:10:04+01:002011-05-16T15:10:22+01:005c281963fa677969b79f0cbbf6d5edc28SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2622.xmlhttp://biolab.isis.rl.ac.uk/uri/89ehttp://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.xml?revision=14160http://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.xml?revision=14161http://biolab.isis.rl.ac.uk/camerons_labblog/14160/I22_29557__b2.xml?revision=141621416314165I22 29558 - b1
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29558
J58001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:11:11+01:002011-05-16T15:11:48+01:005abae496a50867627501787e61ff36a5cSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2624.xmlhttp://biolab.isis.rl.ac.uk/uri/89fhttp://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.xml?revision=14163http://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.xml?revision=14164http://biolab.isis.rl.ac.uk/camerons_labblog/14163/I22_29558__b1.xml?revision=141651416614168I22 29559 - water
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29559
J59001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:12:23+01:002011-05-16T15:12:37+01:005b173dcf12fc22ae8d878214cb569fab4SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2626.xmlhttp://biolab.isis.rl.ac.uk/uri/8a0http://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.xml?revision=14166http://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.xml?revision=14167http://biolab.isis.rl.ac.uk/camerons_labblog/14166/I22_29559__water.xml?revision=141681423614238I22 29589 - A5
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29589
J89001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:34:20+01:002011-05-16T17:34:34+01:005a1cca526ec5f8686a575856cfc7af0ccSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2674.xmlhttp://biolab.isis.rl.ac.uk/uri/8b8http://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.xml?revision=14238http://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.xml?revision=14236http://biolab.isis.rl.ac.uk/camerons_labblog/14236/I22_29589__A5.xml?revision=142371416914171I22 29560 - b4
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29560
J60001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:13:03+01:002011-05-16T15:13:21+01:005e6e38e3758f52d00658cea049a5f1fb3SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2628.xmlhttp://biolab.isis.rl.ac.uk/uri/8a1http://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.xml?revision=14169http://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.xml?revision=14170http://biolab.isis.rl.ac.uk/camerons_labblog/14169/I22_29560__b4.xml?revision=141711417214174I22 29561 - buffer
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29561
J61001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:13:41+01:002011-05-16T15:14:13+01:00590d8775d8c5a3a6d3c38d29e459eee03SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2630.xmlhttp://biolab.isis.rl.ac.uk/uri/8a2http://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.xml?revision=14172http://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.xml?revision=14173http://biolab.isis.rl.ac.uk/camerons_labblog/14172/I22_29561__buffer.xml?revision=141741417514177I22 29562 - b3
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29563
J63001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:14:46+01:002011-05-16T15:14:59+01:005176272ae959aae145fc64a1c18f16c06SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2632.xmlhttp://biolab.isis.rl.ac.uk/uri/8a3http://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.xml?revision=14175http://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.xml?revision=14176http://biolab.isis.rl.ac.uk/camerons_labblog/14175/I22_29562__b3.xml?revision=141771423314235I22 29588 - A6
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29588
J88001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:18:19+01:002011-05-16T17:19:03+01:0053dfeaf5251c72a068e1e57071136731aSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2672.xmlhttp://biolab.isis.rl.ac.uk/uri/8b7http://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.xml?revision=14235http://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.xml?revision=14233http://biolab.isis.rl.ac.uk/camerons_labblog/14233/I22_29588__A6.xml?revision=142341417814180I22 29563 - empty capillary
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29563
J63001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:15:57+01:002011-05-16T15:16:12+01:00528f2bd7c96bfbcb12da7d292eb09a6fdSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2634.xmlhttp://biolab.isis.rl.ac.uk/uri/8a4http://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.xml?revision=14178http://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.xml?revision=14179http://biolab.isis.rl.ac.uk/camerons_labblog/14178/I22_29563__empty_capillary.xml?revision=141801418114183I22 29564 - b2
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29564
J64001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:16:31+01:002011-05-16T15:17:04+01:0057f6cb56eff5970aa4183f8f84cdbd48fSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2638.xmlhttp://biolab.isis.rl.ac.uk/uri/8a5http://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.xml?revision=14181http://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.xml?revision=14182http://biolab.isis.rl.ac.uk/camerons_labblog/14181/I22_29564__b2.xml?revision=141831424514246SAXS on SMALPS #2
Procedures
cameronneylon.netCameron NeylonProcedure: SAXS
Instrument: I22
Project: smalp
following samples were run in 1.5 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
DLPC301029584I22 29584-DLPC
A9301029585I22 29585 - A9
A8301029586I22 29586 A8
A7301029587I22 29587 A7
A6301029588I22 29588 - A6
A5301029589I22 29589 - A5
A4301029590I22 29590 - A4
buffer301029591I22 29591 BUFFER

]]>2011-05-16T21:06:42+01:002011-05-16T21:07:30+01:005a5d72108f78f656089af0d0f053123deSAXSI22smalphttp://biolab.isis.rl.ac.uk/uri/8bbhttp://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.xml?revision=14246http://biolab.isis.rl.ac.uk/camerons_labblog/14245/SAXS_on_SMALPS_2.xml?revision=142451418414186I22 29565 - b1
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29565
J65001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:17:25+01:002011-05-16T15:17:39+01:0053c9ef9c30fd61510a91bf6f20f56fc7cSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2640.xmlhttp://biolab.isis.rl.ac.uk/uri/8a6http://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.xml?revision=14184http://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.xml?revision=14185http://biolab.isis.rl.ac.uk/camerons_labblog/14184/I22_29565__b1.xml?revision=141861423014232I22 29587 A7
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29587
J87001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:17:16+01:002011-05-16T17:17:55+01:00527e203c3c09c356b9a1c5058b16f783cSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2670.xmlhttp://biolab.isis.rl.ac.uk/uri/8b6http://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.xml?revision=14232http://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.xml?revision=14230http://biolab.isis.rl.ac.uk/camerons_labblog/14230/I22_29587_A7.xml?revision=142311418714189I22 29567 - DLPC - lower counts
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29567
J67001.DAT

]]>2011-05-16T15:41:55+01:002011-05-16T15:42:11+01:005b136384342033fd51401d73d9727715dSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2642.xmlhttp://biolab.isis.rl.ac.uk/uri/8a7http://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.xml?revision=14187http://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.xml?revision=14188http://biolab.isis.rl.ac.uk/camerons_labblog/14187/I22_29567__DLPC__lower_counts.xml?revision=141891419014192I22 29574 - A3 - seems ok
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29574
J74001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:52:17+01:002011-05-16T15:52:34+01:005a7a21a17714bc01a369a16afcf3a70f5SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2644.xmlhttp://biolab.isis.rl.ac.uk/uri/8a8http://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.xml?revision=14190http://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.xml?revision=14191http://biolab.isis.rl.ac.uk/camerons_labblog/14190/I22_29574__A3__seems_ok.xml?revision=141921419314195I22 29575 - a2
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29575
J75001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:54:35+01:002011-05-16T15:54:48+01:0058461cfed6474173cd53490da5b02fb59SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2646.xmlhttp://biolab.isis.rl.ac.uk/uri/8a9http://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.xml?revision=14193http://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.xml?revision=14194http://biolab.isis.rl.ac.uk/camerons_labblog/14193/I22_29575__a2.xml?revision=141951419614198I22 29576 - a1
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29576
J76001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:55:25+01:002011-05-16T15:55:41+01:0053dc820cb6739ec705dfd8ad1053fe973SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2648.xmlhttp://biolab.isis.rl.ac.uk/uri/8aahttp://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.xml?revision=14196http://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.xml?revision=14197http://biolab.isis.rl.ac.uk/camerons_labblog/14196/I22_29576__a1.xml?revision=141981422714229I22 29586 A8
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29586
J86001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:16:09+01:002011-05-16T17:16:22+01:005df7cd830e11db0c970b0fa29ed706e97SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2668.xmlhttp://biolab.isis.rl.ac.uk/uri/8b5http://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.xml?revision=14229http://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.xml?revision=14227http://biolab.isis.rl.ac.uk/camerons_labblog/14227/I22_29586_A8.xml?revision=142281419914201I22 29577 - mono-olein
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29577
J77001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T15:56:50+01:002011-05-16T15:57:04+01:005ddb673de2a3da5cf3e04e101b72a707fSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2650.xmlhttp://biolab.isis.rl.ac.uk/uri/8abhttp://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.xml?revision=14199http://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.xml?revision=14200http://biolab.isis.rl.ac.uk/camerons_labblog/14199/I22_29577__monoolein.xml?revision=142011420214204I22 29578 - DMPC
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29578
J78001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:07:28+01:002011-05-16T16:07:42+01:005c2e79939b6be32b3b80e9d96dfe5a7a6SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2652.xmlhttp://biolab.isis.rl.ac.uk/uri/8achttp://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.xml?revision=14202http://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.xml?revision=14203http://biolab.isis.rl.ac.uk/camerons_labblog/14202/I22_29578__DMPC.xml?revision=142041420514207I22 29579 - b9
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29579
J79001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:08:10+01:002011-05-16T16:08:24+01:005ae973ec27baedf9d97e52786df3448dbSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2654.xmlhttp://biolab.isis.rl.ac.uk/uri/8adhttp://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.xml?revision=14205http://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.xml?revision=14206http://biolab.isis.rl.ac.uk/camerons_labblog/14205/I22_29579__b9.xml?revision=142071422414226I22 29585 - A9
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29585
J85001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:15:10+01:002011-05-16T17:15:48+01:005f52c9d32420fa2eff005eb46774aa645SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2666.xmlhttp://biolab.isis.rl.ac.uk/uri/8b4http://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.xml?revision=14226http://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.xml?revision=14224http://biolab.isis.rl.ac.uk/camerons_labblog/14224/I22_29585__A9.xml?revision=142251420814210I22 29580 - b8
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29580
J80001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:14:13+01:002011-05-16T16:14:28+01:005bd451d32cde933e66d34b1489f93b5d1SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2656.xmlhttp://biolab.isis.rl.ac.uk/uri/8aehttp://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.xml?revision=14208http://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.xml?revision=14209http://biolab.isis.rl.ac.uk/camerons_labblog/14208/I22_29580__b8.xml?revision=142101421114213I22 29581 - b7
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29581
J81001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:24:17+01:002011-05-16T16:24:31+01:0056d0469c80f04092ae7d7b2f752da90ecSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2658.xmlhttp://biolab.isis.rl.ac.uk/uri/8afhttp://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.xml?revision=14211http://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.xml?revision=14212http://biolab.isis.rl.ac.uk/camerons_labblog/14211/I22_29581__b7.xml?revision=142131421414216I22 29582 - b6
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29582
J82001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:25:00+01:002011-05-16T16:25:14+01:005c0c68e0b30d93bb07917cbf84b429f6eSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2660.xmlhttp://biolab.isis.rl.ac.uk/uri/8b0http://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.xml?revision=14214http://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.xml?revision=14215http://biolab.isis.rl.ac.uk/camerons_labblog/14214/I22_29582__b6.xml?revision=142161422114223I22 29584-DLPC
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29584
J84001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:14:34+01:002011-05-16T17:14:48+01:00540804bd8238d9d0ab3ff67e8ff013b93SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2664.xmlhttp://biolab.isis.rl.ac.uk/uri/8b3http://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.xml?revision=14223http://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.xml?revision=14221http://biolab.isis.rl.ac.uk/camerons_labblog/14221/I22_29584DLPC.xml?revision=142221421714219I22 29583 - b5
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29583
J83001.DAT

This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-16T16:39:44+01:002011-05-16T16:39:58+01:00547b0b111129ed9268a7f4d354cdfd30cSAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2662.xmlhttp://biolab.isis.rl.ac.uk/uri/8b1http://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.xml?revision=14217http://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.xml?revision=14218http://biolab.isis.rl.ac.uk/camerons_labblog/14217/I22_29583__b5.xml?revision=142191424214244I22 29591 BUFFER
Data
cameronneylon.netCameron NeylonData Type: SAXS_Diamond
Instrument: I22
Project: smalp

Radially averaged but not background subtracted
Raw data fileData file
29591
J91001.DAT

This Post is Linked By: SAXS on SMALPS #2; ]]>2011-05-16T17:35:58+01:002011-05-16T17:36:14+01:0052d67459864ab112fbb032cd5c5ecc3f3SAXS_DiamondI22smalphttp://biolab.isis.rl.ac.uk/data/2678.xmlhttp://biolab.isis.rl.ac.uk/uri/8bahttp://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.xml?revision=14242http://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.xml?revision=14243http://biolab.isis.rl.ac.uk/camerons_labblog/14242/I22_29591_BUFFER.xml?revision=142441425614256B4
Materials
cameronneylon.netCameron NeylonMaterial: Solution
Project: smalp
SMALP sample B4
This Post is Linked By: SAXS on SMALP samples #1; ]]>2011-05-25T11:54:27+01:002011-05-25T11:54:27+01:005b77b3f4a3d949770286d4a04a81aea10Solutionsmalphttp://biolab.isis.rl.ac.uk/uri/8c5http://biolab.isis.rl.ac.uk/camerons_labblog/14256/B4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14256/B4.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14256/B4.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14256/B4.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14256/B4.xml?revision=142561422014257SAXS on SMALP samples #1
Procedures
cameronneylon.netCameron NeylonProcedure: SAXS
Instrument: I22
Project: smalp
following samples were run in 1.5 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.


SampleFramesExposure time I22 Raw Run #Data
AgBeh29544
Water10629552I22-29552
B410629553I22 29553 - b4
buffer10629554I22 29554 - buffer
b310629555I22 29555 - b3
empty cap10629556I22 29556 - empty capillary
b210629557I22 29557 - b2
b110629558I22 29558 - b1
water301029559I22 29559 - water
b4301029560I22 29560 - b4
buffer301029561I22 29561 - buffer
b3301029562I22 29562 - b3
empty301029563I22 29563 - empty capillary
b2301029564I22 29564 - b2
b1301029565I22 29565 - b1
DLPC301029567
A9301029568
A8301029569
A7301029570
A6301029571
A5301029572
A4301029573
A3301029574I22 29574 - A3 - seems ok
A2301029575I22 29575 - a2
A1301029576I22 29576 - a1
mono-olein301029577I22 29577 - mono-olein
DMPC301029578I22 29578 - DMPC
B9301029579I22 29579 - b9
b8301029580I22 29580 - b8
b7301029581I22 29581 - b7
b6301029582I22 29582 - b6
b5301029583I22 29583 - b5

]]>2011-05-16T16:40:47+01:002011-05-25T11:54:58+01:0051567bebfaa4d6e0dfcf88e85b7727ce4SAXSI22smalphttp://biolab.isis.rl.ac.uk/uri/8b2http://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.xml?revision=14220http://biolab.isis.rl.ac.uk/camerons_labblog/14220/SAXS_on_SMALP_samples_1.xml?revision=142571433514380Testing of Superdex 200 5/150 column
Procedure
cameronneylon.netCameron NeylonProcedure: Purification
Have attached the 3mL column to position 7. Slightly more tubing than is ideal but this represents a reasonable "standard" setup. Dissolved a few mgs of GFP in water which means there will be some aggregate and injected 20 uL. Using the Tus buffer as eluent because there was some. At 0.3 mL/min pressure is around 1.2 MPa.

First run seems to indicate that tubing is an issue with peaks coming out later and much broader than in calibration run in brochure (not suprising as we have suboptimal injection, much more tubing etc) but also two peaks from GFP which seem too late for aggregate. So should have put the UV2 on at 490.

Second run, very little signal, probably a problem with injection, this could also be a significant issue with loading on small samples. Obviously much easier with proper injection systems. Suggests first peak is GFP second is solvent breakthrough ( no A490, plausible I guess but seems a strong response for water).

Third run, tried again, this time with the sample spun extensively. See if it shows a difference due to aggregation at all. Might need to heat sample or something to induce more aggregation to test separation and void volume perhaps? Seems to confirm that the first peak is GFP, second is solvent breakthrough. Maybe GFP is just a bit small as a test protein.

Changed to 100 uL loop to check effect of sample load volume. Given peak width suspect that it might not make very much difference. Now see a small aggregate peak at about 1.5 mL which now I know where it is is also in the earlier injection. After that things went badly wrong. Either have overloaded the column badly (but then why get good separation up to that point?) or have injected air onto the column.

After extensive washing tried again with a 100 uL load (53.6 mL). This time seemed to work well. Aggregate peak is separated from the protein peak with near baseline resolution. Resolution doesn't seem to be seriously impaired compared to the 20 uL load (peakwidth of the protein peak is comparable at around 0.8 mL in both cases).

Moved up to a 200uL load (73.7 mL). This is a new (and more dilute) sample. But resolution still seems to be good, a little worse at around (0.8-0.9 mL across the GFP peak, still good resolution from the aggregate).
]]>2011-08-10T15:01:33+01:002011-09-21T15:48:33+01:005c479493f495891adc73afc4f77fbdb6fPurificationhttp://biolab.isis.rl.ac.uk/uri/913http://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.xml?revision=14335http://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.xml?revision=14336http://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.xml?revision=14337http://biolab.isis.rl.ac.uk/camerons_labblog/14335/Testing_of_Superdex_200_5150_column.xml?revision=143801216014495Trans and SANS runs, 22% D2O unliganded Glur0 samples and D2O
Procedures
cameronneylon.netCameron NeylonProcedure: SANS
Instrument: SANS2d
Project: Glur0
The following samples were run as described on SANS2d at ISIS. The samples were run in 1 mm path length rectangular cells (200 uL samples) at a temperature of 20 C.


SampleSANS/TRANSExposure time (uamp.hr)Run #Data
Glur0 in 22% D2O bufferT63346
22% D2O BufferT63347
D2OT63348
D2OS403349
Glur0 in 22% D2O bufferS403350
22% D2O BufferS163351


GCL script
]]>2010-03-10T18:15:38+00:002012-05-11T10:07:40+01:00536d76f94c2de3d615bcc245510a87bd1SANSSANS2dGlur0http://biolab.isis.rl.ac.uk/data/1714.xmlhttp://biolab.isis.rl.ac.uk/data/2841.xmlhttp://biolab.isis.rl.ac.uk/data/2843.xmlhttp://biolab.isis.rl.ac.uk/data/2849.xmlhttp://biolab.isis.rl.ac.uk/uri/5a1http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12161http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12160http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12162http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12163http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12164http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=12746http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=14443http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=14495http://biolab.isis.rl.ac.uk/camerons_labblog/12160/Trans_and_SANS_runs_22_D2O_unliganded_Glur0_samples_and_D2O.xml?revision=144941444514454Buffer Prep for NIMROD Experiment
Procedure
cameronneylon.netCameron Neylon
To make the buffers we will make H2O and D2O version of each and then mix equal volumes. So if we have four salt conditions that requires the making of eight buffers in total. For approx 20 and 200 mM KCl/NaCl in 4 mM naphos buffer the required weights are as follows:


BufferNaH2PO4Na2HPO4NaClKClH2OD2O
D2O 20 mM NaCl0.3119480.32650571.16886880
H2O 20 mM NaCl0.3119480.32650571.16886800
D2O 200 mM NaCl0.3119480.326505711.6886880
H2O 200 mM NaCl0.3119480.326505711.6886800
D2O 20 mM KCl0.3119480.32650571.49103880
H2O 20 mM KCl0.3119480.32650571.49103800
D2O 200 mM KCl0.3119480.326505714.9103880
H2O 200 mM KCl0.3119480.326505714.9103800


The plan is to take 8 x 1L beakers and to weigh each salt in turn by taring the balance on an appropriate scale, with a weight boat in place, the final added salt weight will be recorded in each case. With all salts in place the solvent will be weighed in, if necessary drop by drop which should be accurate enough. All weights will be recorded and be within 0.5% of the target weights. A dried stir bar will then be added to each beaker and the solution dissolved, if necessary over a day or two of stirring.

Ok, that's not going to work because I can't weigh out 0.3g accurately enough. So adjusted plan. Weigh out 2 x 1.3278g and 2 x 1.3060g of the two phosphates and dissolve in 900g and 990 g of H2O and D2O respectively. Then divide the water buffers into 4 x 200g and the D2O into 4 x 220g into beakers already containing the weighed out salt requirements. This means I will need two more beakers!

Actual weights (all in g):


BufferNaH2PO4Na2HPO4H2OD2O
H2O Stock1.327931.30830900.0
D2O Stock1.328051.30873990.1



BufferSaltH2O/D2O stockH2O/D2O
D2O 20 mM NaCl1.16891220.1660.0
H2O 20 mM NaCl1.16880200.0600.0
D2O 200 mM NaCl11.68862220.0660.1
H2O 200 mM NaCl11.68878200.0600.2
D2O 20 mM KCl1.49113220.0660.2
H2O 20 mM KCl1.49122200.0600.0
D2O 200 mM KCl14.91055220.0660.1
H2O 200 mM KCl14.91046200.0]600.9



~0.5 g of lysozyme was weighed out and dissolved into each buffer (~7 mL) which was then dialysed against 3 x 200 mL of the buffer. Due to a mixup there wasn't enough of the H20 20K buffer and this got dialysed o/n against a more concentrated salt buffer.

A fresh buffer was made up for H2O 20K as per the previous method.

Substance addedAmount
NaH2PO41.32793
H2HPO41.30834
H2O to make stock800.0
KCl1.49126
Stock H2O buffer200.0
H2O600.0


Also managed to put the D2O 200K buffer in the wrong sample briefly. Assume that the sample is ok as is now dialysing in correct buffer but probably need to remake enough of the D2O 200K to re-dialyse. Weighed out remaining D2O stock into clean beaker (110.1g). Therefore aim for 7.45527g of KCl (actual 7.45529g) and then add to stock plus 330g of D2O (actual 330.0 g).
Linked Posts
]]>2012-02-22T13:49:10+00:002012-03-01T12:49:38+00:0054483f1cd6e9d267a96a0fb9d5c98a224http://biolab.isis.rl.ac.uk/uri/958http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14445http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14446http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14447http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14448http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14449http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14450http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14451http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14452http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=14453http://biolab.isis.rl.ac.uk/camerons_labblog/14445/Buffer_Prep_for_NIMROD_Experiment.xml?revision=144541447414477Preparation of lysozyme samples for NIMROD
Procedure
cameronneylon.netCameron NeylonProject: NIMROD-March-2012
Lysozyme (8 x ~0.5g) was weighed out and dissolved in 5-10 mL of each of the buffers prepared in Buffer Prep for NIMROD Experiment. Each sample was dialysed against its buffer at least 3 x 200 mL. A mistake was made in preparing the H20 20 K sample and so this buffer was re-made and the sample dialysed a further 3 x 200 mL in the new buffer. Similarly a problem with the D2O 200 K buffer meant that further dialysis was required to be sure that the buffer was correct.

Samples were then centrifuged and supernatant poured off to give:
Lysozyme in H2O 20 mM KCl
Lysozyme in H2O 200 mM KCl
Lysozyme in H20 20 mM NaCl
Lysozyme in H2O 200 mM NaCl
Lysozyme in D2O 20 mM KCl
Lysozyme in D2O 200 mM KCl
Lysozyme in D2O 20 mM NaCl
Lysozyme in D2O 200 mM NaCl
]]>2012-03-03T14:57:38+00:002012-03-03T15:01:38+00:005fd78962de13e64a39c9c50cb1db1f634NIMROD-March-2012http://biolab.isis.rl.ac.uk/uri/96ahttp://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.xml?revision=14476http://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.xml?revision=14477http://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.xml?revision=14475http://biolab.isis.rl.ac.uk/camerons_labblog/14474/Preparation_of_lysozyme_samples_for_NIMROD.xml?revision=1447438614455SAS sample template
Templates
cameron.neylon.myopenid.comCameron NeylonMaterials]] [[Material>Solution]] [[Project>NIMROD-March-2012]]]]>Materials]]
[[Material>Solution]]
[[Project>NIMROD-March-2012]]
]]>2008-11-16T11:19:06+00:002012-03-03T14:29:40+00:0052f7378509d3af8f7769141234a248de9http://biolab.isis.rl.ac.uk/uri/9chttp://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=386http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=389http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=391http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=2781http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=2895http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=2902http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=2925http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=2939http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=11674http://biolab.isis.rl.ac.uk/camerons_labblog/386/SAS_sample_template.xml?revision=144551445614456H20 20mM KCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:30:17+00:002012-03-03T14:30:17+00:00556042b6420918a025c3f9be6af7db5e7http://biolab.isis.rl.ac.uk/uri/959http://biolab.isis.rl.ac.uk/camerons_labblog/14456/H20_20mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14456/H20_20mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14456/H20_20mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14456/H20_20mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14456/H20_20mM_KCl.xml?revision=144561445714457H2O 200 mM KCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:30:30+00:002012-03-03T14:30:30+00:0059aaea4b809823980e7c28fb909df9719http://biolab.isis.rl.ac.uk/uri/95ahttp://biolab.isis.rl.ac.uk/camerons_labblog/14457/H2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14457/H2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14457/H2O_200_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14457/H2O_200_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14457/H2O_200_mM_KCl.xml?revision=144571445814458H20 20mM NaCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:30:40+00:002012-03-03T14:30:40+00:00520dd2530f24f6ea34be7fd8a8428e180http://biolab.isis.rl.ac.uk/uri/95bhttp://biolab.isis.rl.ac.uk/camerons_labblog/14458/H20_20mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14458/H20_20mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14458/H20_20mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14458/H20_20mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14458/H20_20mM_NaCl.xml?revision=144581445914459H2O 200 mM NaCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:31:09+00:002012-03-03T14:31:09+00:005de3fda00c5cac4b70be8e475673d9704http://biolab.isis.rl.ac.uk/uri/95chttp://biolab.isis.rl.ac.uk/camerons_labblog/14459/H2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14459/H2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14459/H2O_200_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14459/H2O_200_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14459/H2O_200_mM_NaCl.xml?revision=144591446014460D2O 20 mM KCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:31:24+00:002012-03-03T14:31:24+00:00541b09bf6a689ce42ad4191f3ce8933c5http://biolab.isis.rl.ac.uk/uri/95dhttp://biolab.isis.rl.ac.uk/camerons_labblog/14460/D2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14460/D2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14460/D2O_20_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14460/D2O_20_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14460/D2O_20_mM_KCl.xml?revision=144601446114461D2O 200 mM KCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:31:44+00:002012-03-03T14:31:44+00:005c4f24a9713a4ee1091f7c182d3bc9b4fhttp://biolab.isis.rl.ac.uk/uri/95ehttp://biolab.isis.rl.ac.uk/camerons_labblog/14461/D2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14461/D2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14461/D2O_200_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14461/D2O_200_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14461/D2O_200_mM_KCl.xml?revision=144611446214462D2O 20 mM NaCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:32:03+00:002012-03-03T14:32:03+00:005d1846f47b397adb609e5581a69eb65c1http://biolab.isis.rl.ac.uk/uri/95fhttp://biolab.isis.rl.ac.uk/camerons_labblog/14462/D2O_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14462/D2O_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14462/D2O_20_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14462/D2O_20_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14462/D2O_20_mM_NaCl.xml?revision=144621446314463D2O 200 mM NaCl
Materials
cameronneylon.netCameron Neylon ]]>2012-03-03T14:32:14+00:002012-03-03T14:32:14+00:005c972c19c7d3f5ee074a3133adf6f5581http://biolab.isis.rl.ac.uk/uri/960http://biolab.isis.rl.ac.uk/camerons_labblog/14463/D2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14463/D2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14463/D2O_200_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14463/D2O_200_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14463/D2O_200_mM_NaCl.xml?revision=144631447314473UV-Vis of Lysozyme samples
Procedure
cameronneylon.netCameron NeylonProcedure: UV-Vis
Each sample was diluted 5 uL plus 495 uL of buffer and the UV-Vis Spectrum obtained.


SampleData
Lysozyme in H2O 20 mM KCl
Lysozyme in H2O 200 mM KCl
Lysozyme in H20 20 mM NaCl
Lysozyme in H2O 200 mM NaCl
Lysozyme in D2O 20 mM KCl
Lysozyme in D2O 200 mM KCl
Lysozyme in D2O 20 mM NaCl
Lysozyme in D2O 200 mM NaCl

]]>2012-03-03T14:42:11+00:002012-03-03T14:42:11+00:005c2b16ed0367804d0b314a1b9f348767cUV-Vishttp://biolab.isis.rl.ac.uk/uri/969http://biolab.isis.rl.ac.uk/camerons_labblog/14473/UVVis_of_Lysozyme_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14473/UVVis_of_Lysozyme_samples.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14473/UVVis_of_Lysozyme_samples.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14473/UVVis_of_Lysozyme_samples.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14473/UVVis_of_Lysozyme_samples.xml?revision=144731446614479Lysozyme in H20 20 mM NaCl
Materials
cameronneylon.netCameron Neylon
For 2mL at 25 mg/mL require 1.341 mL
Linked Posts
]]>2012-03-03T14:33:08+00:002012-03-03T15:32:03+00:005c6f0de4e57f85cf10e2fa08d229413adhttp://biolab.isis.rl.ac.uk/uri/963http://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.xml?revision=14466http://biolab.isis.rl.ac.uk/camerons_labblog/14466/Lysozyme_in_H20_20_mM_NaCl.xml?revision=144791447114483Lysozyme in D2O 200 mM NaCl
Materials
cameronneylon.netCameron Neylon
Required for 2 mL of 25 mg/mL: 0.992 mL
Linked Posts
]]>2012-03-03T14:34:31+00:002012-03-03T15:35:57+00:005dd6101df58798e4b8ff850fac96b0cf8http://biolab.isis.rl.ac.uk/uri/968http://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.xml?revision=14471http://biolab.isis.rl.ac.uk/camerons_labblog/14471/Lysozyme_in_D2O_200_mM_NaCl.xml?revision=144831447014484Lysozyme in D2O 20 mM NaCl
Materials
cameronneylon.netCameron Neylon
For 2mL of 25 mg/mL require 1.018 mL
Linked Posts
]]>2012-03-03T14:34:15+00:002012-03-03T15:49:46+00:00513bdc7b879ac3dc76ad059fc8a12f33chttp://biolab.isis.rl.ac.uk/uri/967http://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.xml?revision=14470http://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.xml?revision=14482http://biolab.isis.rl.ac.uk/camerons_labblog/14470/Lysozyme_in_D2O_20_mM_NaCl.xml?revision=144841446714485Lysozyme in H2O 200 mM NaCl
Materials
cameronneylon.netCameron Neylon
For 2mL of 25 mg/mL require 1.152 mL
Linked Posts
]]>2012-03-03T14:33:20+00:002012-03-03T15:51:17+00:005f5d281eb139b1cd546576248d9c32c1ahttp://biolab.isis.rl.ac.uk/uri/964http://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.xml?revision=14467http://biolab.isis.rl.ac.uk/camerons_labblog/14467/Lysozyme_in_H2O_200_mM_NaCl.xml?revision=144851446914488Lysozyme in D2O 200 mM KCl
Materials
cameronneylon.netCameron Neylon
Required for 2mL of 25 mg/mL: 1.634 mL

Required for 3mL of 10 mg/mL: 0.980 mL
Linked Posts
]]>2012-03-03T14:33:56+00:002012-03-04T16:20:08+00:0059aec27fbdfd790c2197e21d8c8aa4768http://biolab.isis.rl.ac.uk/uri/966http://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.xml?revision=14469http://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.xml?revision=14481http://biolab.isis.rl.ac.uk/camerons_labblog/14469/Lysozyme_in_D2O_200_mM_KCl.xml?revision=144881446814489Lysozyme in D2O 20 mM KCl
Materials
cameronneylon.netCameron Neylon
Required for 2 mL of 25 mg/mL: 1.976 mL

Required for 3 mL of 10 mg/mL: 1.186 mL
Linked Posts
]]>2012-03-03T14:33:38+00:002012-03-04T16:20:51+00:005335e440634584f22adf1ba9f5c25c6d2http://biolab.isis.rl.ac.uk/uri/965http://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.xml?revision=14468http://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.xml?revision=14480http://biolab.isis.rl.ac.uk/camerons_labblog/14468/Lysozyme_in_D2O_20_mM_KCl.xml?revision=144891446514490Lysozyme in H2O 200 mM KCl
Materials
cameronneylon.netCameron Neylon
For 2mL of 25 mg/mL require 1.285 mL

Required for 3 mL of 10 mg/mL: 0.771 mL
Linked Posts
]]>2012-03-03T14:32:54+00:002012-03-04T16:21:35+00:005814abccd2a68decd186baf5457e9c230http://biolab.isis.rl.ac.uk/uri/962http://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.xml?revision=14465http://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.xml?revision=14486http://biolab.isis.rl.ac.uk/camerons_labblog/14465/Lysozyme_in_H2O_200_mM_KCl.xml?revision=144901446414491Lysozyme in H2O 20 mM KCl
Materials
cameronneylon.netCameron Neylon
To make 2mL at 25 mg/mL require 1.453 mL

For 3 mL of 10 mg/mL: 0.872 mL
Linked Posts
]]>2012-03-03T14:32:39+00:002012-03-04T16:22:25+00:005f772b2bc269cb7a07eb1fc2293455ccdhttp://biolab.isis.rl.ac.uk/uri/961http://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.xml?revision=14464http://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.xml?revision=14472http://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.xml?revision=14478http://biolab.isis.rl.ac.uk/camerons_labblog/14464/Lysozyme_in_H2O_20_mM_KCl.xml?revision=144911448714493Calculation of volume excluded by lysozyme
Procedure
cameronneylon.netCameron Neylon
The molecular formula for lysozyme (calculated at ExPASy with ProtParam program) is C705H1116N214O204S12 (2251 atoms total, MW is 16238.6)

So as to use the same numbers as have been calculated for the buffers we will just assume 2% as our densities and concentrations are not accurate enough to justify higher precision.

So for each atom I need 2% of the molar atomic composition of the protein as follows:


Atommols in 1 mol lysmolar proportion2% amount(25 mg/mL)10mg/mL5 mg/mL2 mg/mL
C7050.3130.006260.002500.001255E-4
H11160.4960.009920.0039680.001997.9E-4
N2140.09510.001900.000763.8E-41.52E-4
O2040.09060.001810.0007243.62E-41.5E-4
S120.005330.0001070.00004282.14E-58.6E-6
]]>2012-03-04T12:52:37+00:002012-03-07T10:46:09+00:005754327b712ad3c33c5dbd2118ab141fahttp://biolab.isis.rl.ac.uk/uri/96bhttp://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.htmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.xmlhttp://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.pnghttp://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.xml?revision=14487http://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.xml?revision=14492http://biolab.isis.rl.ac.uk/camerons_labblog/14487/Calculation_of_volume_excluded_by_lysozyme.xml?revision=14493